EMAGE mouse gene expression spatial database

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EMAGE:7684

Ppp1r2 protein phosphatase 1, regulatory (inhibitor) subunit 2 ( MGI:1914099)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009514. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009514_01	euxassay_009514_02	euxassay_009514_03	euxassay_009514_04

euxassay_009514_05	euxassay_009514_06	euxassay_009514_07	euxassay_009514_08	euxassay_009514_09

euxassay_009514_10	euxassay_009514_11	euxassay_009514_12	euxassay_009514_13	euxassay_009514_14

euxassay_009514_15	euxassay_009514_16	euxassay_009514_17	euxassay_009514_18	euxassay_009514_19

euxassay_009514_20	euxassay_009514_21	euxassay_009514_22	euxassay_009514_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7684_wholemount_strong.wlz

7684_wholemount_moderate.wlz

7684_wholemount_weak.wlz

7684_wholemount_possible.wlz

7684_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7684_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 02 03 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 17 18 moderate expression: see section 06

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 06 17 weak expression: see section 18

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 11 12 13 14 15 16 17

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 12 16 17 moderate expression: see section 06 07 08 09 11 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36887
Entity Detected:	Ppp1r2, protein phosphatase 1, regulatory (inhibitor) subunit 2 ( MGI:1914099)
Sequence:	sense strand is shown >T36887 ACTCGGGAGCAAGAAAGTAGTGGAGAGGAAGATAATGACCTCTCCCCTGAGGAGCGAGAAAAGAAGCGAC AGTTTGAAATGAAAAGGAAGCTTCATTACAATGAAGGACTCAATATTAAGTTAGCCAGACAGTTAATTTC AAAAGACCTACATGATGATGATGAAGATGAAGAAATGGCAGAGACTGCAGATGGAGACAGCATGAATGTT GAAGAGTCAAGTCAAGGATCTACTACAAGTGACCATCTGCAACACAAATCACAAAGTTCATAAAAGAAAC ATGTTCAACAATGCGGTCGTTGTCAAATACAAACCTTGTTACTATACTACACTGTTTCTTGTTCTCTACA ATTCATGACTTATGTACCAGAGTACACACCAGTTATATATTGCCAAGAATTAAGACCATAGAGACTGAGT AGATGGAAAATGCCTAATTAATACATATATTCTTGTGCCTCATATTTCACCACAAGTACAGTGTACTGTT AGTAGTCCAAAACTGCTTTACTTTTGTAAAAACACTGGTTAATTTGTATAAGATATTATATAGCTTTTTA TGCTTTAGAGATTAAATATCATGGGGGAAACTAATTTATTTTTGTCACTTCTAGATGGTGGTACCTCTTA TAAAATGTTTGGGGGTAGTTTGATGTCATTTCTTCTTTTAATCAAAAGCCTATTCACAAAGTGGGATATA TAGATGGATAAATATTTAGGCTGAAAGAATTTAACTTTTATCTTTACCTTGTTTTGGCTAATTGAAAAGG TTTCTAAGAAGTTTTGTTCATTTTCTGATCATTTAAAATTCCTGTTGATTTTCTTTGTGCTTGAAAGGAA TAAAATCTTTTTATCCAAACTGGTTTCCCCCATTCCCACCCCAAATAAATACACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91141. Forward Primer - name:091141_F_cDNA_Ppp1r2, sequence:ACTCGGGAGCAAGAAAGTAGTG; Reverse Primer - name:091141_N_SP6_cDNA_Ppp1r2, sequence:TGTGTATTTATTTGGGGTGGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7687 same embryo
	EMAGE:7683 same embryo
	EMAGE:7685 same embryo
	EMAGE:7686 same embryo
	EMAGE:7682 same embryo
	EurExpress:euxassay_009514 same experiment
	MGI:4827375 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source