EMAGE mouse gene expression spatial database

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EMAGE:7687

Ppp1r1c protein phosphatase 1, regulatory (inhibitor) subunit 1C ( MGI:1923185)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009513. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009513_01	euxassay_009513_02	euxassay_009513_03	euxassay_009513_04

euxassay_009513_05	euxassay_009513_06	euxassay_009513_07	euxassay_009513_08	euxassay_009513_09

euxassay_009513_10	euxassay_009513_11	euxassay_009513_12	euxassay_009513_13	euxassay_009513_14

euxassay_009513_15	euxassay_009513_16	euxassay_009513_17	euxassay_009513_18	euxassay_009513_19

euxassay_009513_20	euxassay_009513_21	euxassay_009513_22	euxassay_009513_23	euxassay_009513_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7687_wholemount_strong.wlz

7687_wholemount_moderate.wlz

7687_wholemount_weak.wlz

7687_wholemount_possible.wlz

7687_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7687_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 15 weak expression: see section 16

pons mantle layer

moderate

regional

moderate expression: see section 07 18

facial vii ganglion

strong

regional

strong expression: see section 21 22 moderate expression: see section 04 20 weak expression: see section 05

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 18 19 moderate expression: see section 06 07

trigeminal v ganglion

strong

regional

strong expression: see section 03 05 18 19 21 22 moderate expression: see section 04 06 07 08 09 17 20

vagus x ganglion

strong

regional

strong expression: see section 18 19 moderate expression: see section 07 08 17

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05 06 07 08 17 18 19

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 12 13 14 15 16 17 moderate expression: see section 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36886
Entity Detected:	Ppp1r1c, protein phosphatase 1, regulatory (inhibitor) subunit 1C ( MGI:1923185)
Sequence:	sense strand is shown >T36886 CTGCCACATGAATGTTCTCACTTTGATACTGCCAAAGCTTAAGTCCTCCTGGCCTACGACATAATTTACA TTCTAAGGTCAAATGTAGAGAGCATTACAAAATAAATAGCAAATGTGTGTGGGACGAGTGTGAGTGGGGA TTAATGGAAGACGGGCATCTGCGGAGGCTCCTAAGTAATAACCGCACTTAGCAAGCTTCTGTGCCCTACA TCAGACCCCAGTTTACCTCAAGGAGACAGTGATAGGCAGACATCTGTTGTCTGGCTTCAAAACACCACAT CAGATGTTACATGATTTCCATCCAGCAGTTACTTAAAGAAACAAATGTCCCTTTATTTTCTAAGCCATTC AATGTTGGCTTCTCAGCATCATCTAGTTATTGGAAAGGAAATGTTAAATTTGTAAGTAAACATAGCCATT CTCTTCTGCCTTTTCTTTATTATTATTTTTTAACTATTTAAAACGAAGCCATCTATATTGTATCACTTTT GTTTGTCTGGGTTTTTCATTGTTTCTTTCTGTTTTATTGGTGCTAGAGAATGAACCCAGGGCTTTGTATA TGCAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70157. Forward Primer - name:070157_F_cDNA_Ppp1r1c, sequence:CTGCCACATGAATGTTCTCACT; Reverse Primer - name:070157_N_SP6_cDNA_Ppp1r1c, sequence:TTGCATATACAAAGCCCTGGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7684 same embryo
	EMAGE:7683 same embryo
	EMAGE:7685 same embryo
	EMAGE:7686 same embryo
	EMAGE:7682 same embryo
	EurExpress:euxassay_009513 same experiment
	MGI:4827374 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source