EMAGE mouse gene expression spatial database

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EMAGE:7688

Idh1 isocitrate dehydrogenase 1 (NADP+), soluble ( MGI:96413)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005308. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005308_01	euxassay_005308_02	euxassay_005308_03	euxassay_005308_04

euxassay_005308_05	euxassay_005308_06	euxassay_005308_07	euxassay_005308_08	euxassay_005308_09

euxassay_005308_10	euxassay_005308_11	euxassay_005308_12	euxassay_005308_13	euxassay_005308_14

euxassay_005308_15	euxassay_005308_16	euxassay_005308_17	euxassay_005308_18	euxassay_005308_19

euxassay_005308_20	euxassay_005308_21	euxassay_005308_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7688_wholemount_strong.wlz

7688_wholemount_moderate.wlz

7688_wholemount_weak.wlz

7688_wholemount_possible.wlz

7688_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7688_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 16 17 18 19 moderate expression: see section 20

adrenal gland

strong

regional

strong expression: see section 04 05 06 11 12 13 14

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vibrissa

moderate

regional

moderate expression: see section 03 04 05 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 15 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 15 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 06 07 14

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 16

trigeminal v nerve

strong

regional

strong expression: see section 08 14

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12

cervico-thoracic ganglion

strong

regional

strong expression: see section 07 12

cervical ganglion

strong

regional

strong expression: see section 07 14

thoracic ganglion

strong

regional

strong expression: see section 09

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 11 12 13 14 15

not examined

regional

not examined expression: see section 01 02 20 21 22

neural retina

strong

regional

strong expression: see section 01 02 20 21 22

viscerocranium

strong

regional

Expression in the turbinate bone.

testis

moderate

regional

moderate expression: see section 04 05 15 16 17

clavicle

moderate

regional

moderate expression: see section 07 08 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8961
Entity Detected:	Idh1, isocitrate dehydrogenase 1 (NADP+), soluble ( MGI:96413)
Sequence:	sense strand is shown >T8961 ACAAAGCCGGGAAGAGTTAAACAGCCTTGACCCTGCCGCTGGGCTTCCGAGCGAAGGTTGTGGCAGATGC TGATCTAACTGGGGCCGGCTTATTACAGCTTGTGTGTACGCGCGGGTGTGAGCCGGGTTATTGAAGTAAA AATGTCCAGAAAAATCCAAGGAGGTTCTGTGGTGGAGATGCAAGGAGATGAAATGACACGAATCATTTGG GAATTGATTAAGGAAAAACTTATTCTTCCCTATGTGGAACTGGATCTGCATAGCTATGATTTAGGCATAG AGAATCGTGATGCCACCAATGACCAGGTCACCAAAGATGCTGCAGAGGCTTTAAAGAAATACAACGTGGG CGTCAAGTGTGCTACCATCACCCCCGATGAGAAGAGGGTTGAAGAATTCAAGTTGAAACAAATGTGGAAA TCCCCAAATGGCACCATCCGAAACATTCTGGGTGGCACTGTCTTCAGGGAAGCTATTATCTGCAAAAATA TCCCCCGGCTAGTGACAGGCTGGGTAAAACCCATCATCATTGGCCGACATGCATATGGGGACCAATACAG AGCAACTGATTTTGTTGTTCCTGGGCCTGGAAAAGTAGAGATAACCTACACACCAAAAGATGGAACTCAG AAGGTGACATACATGGTACATGACTTTGAAGAAGGTGGTGGTGTTGCCATGGGCATGTACAACCAGGATA AGTCAATTGAAGACTTTGCACACAGTTCCTTCCAAATGGCTCTGTCCAAGGGCTGGCCTTTGTATCTCAG CACCAAGAACACTATTCTGAAGAAGTATGATGGGCGTTTCAAAGACATCTTCCAGGAGATCTATGACAAG AAATACAAGTCCCAGTTTGAAGCTCAGAAGATCTGCTATGAACACAGGCTCATAGATGACATGGTGGCCC AAGCTATGAAGTCCGAGGGAGGCTTCATCTGGGCCTGTAAGAATTACGATGGGGATGTGCAGTCAGACTC AGTCGCCCAAGGTTATGGCTCCCTTGGCATGATGACCAGTGTGCTGATTTGTCCAGATGGTAAGACGGTA GAAGCAGAGGCTGCCCATGGCACTGTCACACGTCACTACCGCATGTACCAGAAAGGGCAAGAGACGTCCA CCAACCCCATTGCTTCCATTTTTGCCTGGTCCCGAGGGTTAGCCCACAGAGCAAAGCTTGATAACAATAC TGAGCTCAGCTTCTTCGCAAAGGCTTTGGAAGACGTCTGCATTGAGACCATTGAGGCTGGCTTTATGACT AAGGACTTGGCTGCTTGCATTAAAGGCTTACCCAATGTACAACGTTCTGACTACTTGAATACATTTGAGT TTATGGACAAACTTGGAGAAAACTTGAAGGCCAAATTAGCTCAGGCCAAACTTTAAGGTCAAACCTGGGC TTAGAATGAGTCTTTGCGGTAACTAGGTCCACAGGTTTACGTATTTTTTTTTTTTTTTTTAGTAACACTC AAGATTAAAAACAAAAATCATTTTGTAATTTGTTTAGAAGACAAAGTTGAACTTTTATATATGTTTACAG TCTTTTTTCTTTTTCATACAGTTATTGCCACCTTAATGAATGTGGTGGGGAAATTTTTTTAATTGTATTT TATTGTGTAGTAGCAGTGTAGGAATTATGTTAGTACCTGTTCACAATTAACTGTCATGTTTTCTCATGCT CTAATGTAAATGACCAAAATCAGAAGTGCTCCAAGGGTGAACAATAGCTACAGTATGGTTCCCCATAAGG GGAAAAGAGAAACTCACTTCCCCTGTTGTCCATGAGTGTGAACACTGGGGCCTTTGTACGCAAATGTTGT ACTGTGTGTGGGAGAGCTATACAGTAAGCTCACATAAGACTGGAACAGATAGGATGTGTGTAGCTAAAAT GCATGGCAGACGTGTTTATAAAGAGCATGTATGTGTCCAATATACTAGTTATATTTTAAGACCACTGGAG AATTCCAAGTCTAGAATAAATGCAGACTGGAGGATTCTGCTCTTTGATTTCTCTTCTCCTGTGACCCAGC CTAAGTATTATCCTACCCCAAGCAGTACATTTCACCCATGGGCAATAATGGGAGCTGTACCGTTTGGATT TCTGCTGACCTGCTGCATTTCTTTTATATAAATGTGACTTTTTTTTCCCAGAAGTTGATATTAAACACTA TTCCAGTCTAGTCCTTCTAAACTGTTAATTTTAATTAAAATGAAGTACTAATGAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6808731 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6808731 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7692 same embryo
	EMAGE:7691 same embryo
	EMAGE:7693 same embryo
	EMAGE:7690 same embryo
	EMAGE:7689 same embryo
	EurExpress:euxassay_005308 same experiment
	MGI:4825500 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source