EMAGE mouse gene expression spatial database

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EMAGE:7689

Hsp90ab1 heat shock protein 90 alpha (cytosolic), class B member 1 ( MGI:96247)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007468_01	euxassay_007468_02	euxassay_007468_03	euxassay_007468_04	euxassay_007468_05

euxassay_007468_06	euxassay_007468_07	euxassay_007468_08	euxassay_007468_09	euxassay_007468_10

euxassay_007468_11	euxassay_007468_12	euxassay_007468_13	euxassay_007468_14	euxassay_007468_15

euxassay_007468_16	euxassay_007468_17	euxassay_007468_18	euxassay_007468_19	euxassay_007468_20

euxassay_007468_21	euxassay_007468_22	euxassay_007468_23	euxassay_007468_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7689_wholemount_strong.wlz

7689_wholemount_moderate.wlz

7689_wholemount_weak.wlz

7689_wholemount_possible.wlz

7689_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7689_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8957
Entity Detected:	Hsp90ab1, heat shock protein 90 alpha (cytosolic), class B member 1 ( MGI:96247)
Sequence:	sense strand is shown >T8957 CCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGGACTTGGTCCGGGCCCACCACCCTGCTCTGTACTACT ACTCGGCTTTCCCGTCAAGATGCCTGAGGAAGTGCACCATGGAGAGGAGGAGGTGGAGACCTTTGCCTTT CAGGCAGAAATTGCCCAGCTCATGTCCCTCATCATCAACACTTTCTATTCAAACAAGGAGATTTTCCTCC GCGAGTTGATCTCTAATGCTTCAGATGCCCTGGACAAGATTCGATATGAGAGCCTGACGGACCCTTCTAA GTTGGACAGTGGGAAAGAGCTGAAAATTGACATCATCCCCAACCCTCAGGAGCGCACGCTGACTTTGGTG GACACAGGCATTGGCATGACCAAGGCTGACCTCATTAATAACCTGGGAACCATTGCTAAGTCTGGCACGA AGGCGTTCATGGAGGCTCTCCAGGCTGGTGCAGACATCTCCATGATCGGGCAGTTTGGTGTCGGATTCTA CTCGGCCTATCTAGTTGCAGAGAAAGTGGTTGTGATCACGAAGCACAATGATGATGAGCAGTATGCCTGG GAGTCGTCTGCGGGTGGCTCCTTCACCGTCCGGGCAGACCATGGTGAGCCCATTGGCCGGGGTACCAAAG TGATCCTTCACCTCAAAGAAGACCAGACAGAGTACTTGGAGGAGAGGAGGGTCAAGGAAGTGGTGAAGAA ACATTCGCAGTTCATAGGCTATCCCATCACCCTCTATTTGGAGAAGGAACGGGAGAAGGAGATCAGTGAT GATGAGGCAGAGGAAGAGAAAGGTGAGAAAGAGGAGGAAGATAAGGAGGATGAGGAGAAGCCTAAGATTG AAGATGTGGGATCCGATGAGGAAGATGACAGCGGCAAAGACAAGAAAAAGAAAACAAAGAAGATCAAAGA GAAGTACATTGACCAGGAGGAGCTGAACAAGACAAAGCCTATCTGGACCAGAAACCCGGATGACATCACG CAGGAGGAGTATGGCGAATTCTATAAGAGCCTCACCAATGACTGGGAGGACCACTTGGCAGTCAAGCACT TCTCTGTAGAAGGTCAGTTGGAATTCAGGGCATTGCTCTTCATTCCCCGGCGGGCTCCCTTCGACCTTTT TGAGAACAAGAAGAAGAAGAACAACATCAAATTGTATGTCCGCCGTGTGTTCATCATGGACAGCTGTGAC GAGCTGATACCTGAGTACCTCAACTTTATCCGCGGTGTGGTTGACTCCGAGGACCTGCCCCTGAACATCT CCCGGGAGATGCTGCAGCAGAGCAAGATCCTGAAGGTCATCCGCAAGAACATCGTCAAGAAGTGCCTGGA GCTCTTCTCCGAGCTGGCTGAGGACAAGGAGAACTACAAGAAGTTCTATGAGGCCTTCTCCAAAAATTTA AAGCTTGGAATTCATGAAGATTCCACTAACCGACGCCGCCTCTCTGAGCTCCTTCGCTATCACACCTCTC AGTCTGGAGATGAGATGACCTCCTTGTCAGAGTATGTGTCTCGCATGAAGGAGACCCAGAAGTCCATCTA CTATATCACTGGTGAGAGCAAAGAGCAAGTGGCCAACTCTGCCTTTGTGGAGCGAGTGCGGAAGCGGGGC TTCGAGGTGGTGTATATGACTGAGCCTATTGACGAGTACTGCGTGCAGCAGCTCAAGGAGTTTGATGGGA AGAGCCTGGTCTCAGTGACTAAGGAGGGCCTGGAGCTACCAGAGGACGAGGAAGAGAAGAAGAAAATGGA GGAGAGCAAGGCAAAGTTTGAGAATCTCTGCAAGCTCATGAAGGAGATCTTGGACAAGAAGGTTGAAAAG GTGACAATCTCCAATAGGCTTGTGTCTTCACCCTGCTGCATTGTGACAAGCACCTATGGCTGGACAGCCA ACATGGAACGGATCATGAAGGCCCAGGCACTGCGAGACAACTCTACAATGGGCTACATGATGGCCAAAAA ACACCTGGAGATCAACCCTGACCACCCCATCGTGGAGACCCTGCGGCAGAAGGCTGAGGCAGACAAAAAC GACAAAGCTGTCAAGGACCTGGTGGTGCTGCTGTTTGAAACTGCTCTGCTCTCCTCTGGTTTCTCACTTG AGGATCCCCAAACCCACTCCAACCGCATCTACCGCATGATTAAACTAGGCCTGGGCATCGATGAAGATGA GGTCACTGCAGAGGAGCCCAGTGCTGCTGTTCCTGATGAGATCCCCCCTCTGGAAGGCGATGAGGATGCC TCGCGCATGGAAGAGGTGGATTAAAGCCTCCTGGAAGAAGCCCTGCCCTCTGTATAGTATCCCCGTGGCT CCCCCAGCAGCCCTGACCCACCTGGCTCTCTGCTCATGTCTACAAGAATCTTCTATCCTGTCCTGTGCCT TAAGGCAGGAAGATCCCCTCCCACAGAATAGCAGGGTTGGGTGTTATGTATTGTGGTTTTTTTGTTTGTT TTATTTTGTTCTAAAATTAAAAGTATGCAAAATAAAGAAGATGCAGTTTTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6808699 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6808699 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7692 same embryo
	EMAGE:7691 same embryo
	EMAGE:7693 same embryo
	EMAGE:7688 same embryo
	EMAGE:7690 same embryo
	EurExpress:euxassay_007468 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source