EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7691

BC005764 cDNA sequence BC005764 ( MGI:2388640)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005299. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005299_01	euxassay_005299_02	euxassay_005299_03	euxassay_005299_04

euxassay_005299_05	euxassay_005299_06	euxassay_005299_07	euxassay_005299_08	euxassay_005299_09

euxassay_005299_10	euxassay_005299_11	euxassay_005299_12	euxassay_005299_13	euxassay_005299_14

euxassay_005299_15	euxassay_005299_16	euxassay_005299_17	euxassay_005299_18	euxassay_005299_19

euxassay_005299_20	euxassay_005299_21	euxassay_005299_22	euxassay_005299_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7691_wholemount_strong.wlz

7691_wholemount_moderate.wlz

7691_wholemount_weak.wlz

7691_wholemount_possible.wlz

7691_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7691_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 03 18 19 moderate expression: see section 04 05 06

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 17 moderate expression: see section 05 06

trigeminal v ganglion

strong

regional

strong expression: see section 02 17 18 19 21 moderate expression: see section 03 04 05 06 07 08 16 20

vagus x ganglion

strong

regional

strong expression: see section 06 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 17 18 19 moderate expression: see section 05 06 07 16 20

trigeminal v nerve

strong

regional

strong expression: see section 09 17 moderate expression: see section 08 15 16

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 not examined expression: see section 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 14 moderate expression: see section 08 12 13

cervical ganglion

strong

regional

strong expression: see section 16 moderate expression: see section 07 15

thoracic ganglion

moderate

regional

moderate expression: see section 09 10

dorsal root ganglion

strong

regional

strong expression: see section 06 07 12 13 14 15 16

neural retina

moderate

regional

moderate expression: see section 01 02 03 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 14 moderate expression: see section 09 10 11 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8959
Entity Detected:	BC005764, cDNA sequence BC005764 ( MGI:2388640)
Sequence:	sense strand is shown >T8959 CTGCTCCAGCCTCAGTTTCCCCGAGCCCTCTTGGCTAACCCTTATCTCCCCACCTGGAGACTCCATCTGC GCAGCTCCTAGTCTCCAGCCTGCCTTTGTCCCCCTGCAATCTCGGCCTACAGCGCCAACGTCACCATGCT TGCTATGAAGGAGAAGAATAAAACGCCGAAGGACAGCATGACACTCCTGCCTTGCTTTTATTTCGTGGAG CTACCCATCGTGGCTTCCTCCATTGTGTCTCTGTACTTCCTGGAGCTGACTGACCTGTTCAAGCCGGCCA AGGTGGGCTTTCAGTGCTACGACCGCGCCCTGTCCATGCCCTATGTGGAGACAAATGAAGAGCTGATCCC ATTGCTCATGCTGCTGAGCCTGGCCTTCGCTGCACCTGCGGCCTCAATCATGGTCGGCGAGGGCATGGTC TACTGTCTGCAGTCCAGGCTCTGGGGCCGTGGTCCAGGGGGCGTGGAGGGCAGCATCAACGCTGGTGGCT GCAACTTCAACTCCTTCCTCCGGCGCACAGTGCGCTTTGTGGGTGTACACGTGTTTGGCCTGTGTGCCAC GGCTCTGGTGACAGATGTCATCCAGCTGGCCACCGGCTACCACACACCTTTCTTCTTAACGGTCTGCAAA CCCAATTACACCCTGCTGGGCACTTCCTGTGAGTCGAACCCTTACATCACACAGGACATCTGCTCTGGCC ACGATACCCATGCCATCCTGTCAGCAAGGAAGACCTTCCCGTCCCAGCACGCCACTCTGTCAGCCTTTGC TGCTGTCTACGTGTCGATGTACTTCAACGCGGTTATCTCGGACACCACGAAGCTGCTGAAGCCCATCCTT GTGTTTGCCTTCGCCATTGCTGCGGGCGTCTGCGGCCTCACACAGATCACCCAGTATCGAAGCCACCCTG TGGACGTCTACGCTGGCTTTCTTATCGGTGCTGGCATCGCTGCCTACCTGGCCTGCCACGCTGTGGGCAA CTTCCAGGCACCACCTGCAGAAAAGGTTCCTACCCCAGCTCCTGCCAAGGACGCCCTGCGAGCGCTGACA CAGCGGGGCCATGAGTCCATGTATCAGCAGAATAAGTCTGTCAGTACCGATGAGCTGGGCCCTCCAGGGA GGCTAGAGGGCGTGCCTCGGCCTGTGGCTCGAGAGAAGACATCTCTTGGCAGCCTGAAGCGAGCCAGCGT GGATGTGGACCTGCTGGCCCCACGTAGCCCCATGGGCAAAGAAGGCATGGTCACCTTCAGCAACACACTG CCCCGGGTCAGCACGCCCTCGCTGGATGACCCTGCACGGCGCCACATGACTATCCACGTGCCCCTTGATG CCTCCCGTTCCAGGCAGCTTATCGGTGAGTGGAAGCAAAAATCCCTGGAGGGACGTGGCCTGGGTCTGCC TGATGAAGCCAGCCCTGTGCATCTGAGGGCCCCAGCAGAGCAGGTAGCAGAGGAGGAAGAGGAAGAGGAG GAGGAGGAGGAAGAAGAGGAGGAAGAGGAGGAAGAGGAAGGGCCTGTTCCACCCTCACTCTACCCTACTG TCCAGGCTCGGCCAGGCCTTGGGCCCCGAGTCATCCTGCCTCCAAGGCCAGGGCCCCAGCCCCTCGTACA CATCCCTGAGGAAGGCGTTCAGGCTGGAGCTGGCCTGTCACCCAAGAGCAGCAGCTCATCAGTGAGGGCC AAGTGGCTGTCAATGGCTGAGAAGGGTGGGGGCCCAGTGGCTGTGGCTCCATCCCAGCCCCGGGTGGCCA ACCCACCCAGGCTACTACAGGTCATCGCTATGTCCAAGGCGGCAGGGGGCCCCAAGGCTGAGACAGCTTC GTCCTCCAGTGCCAGCTCCGACTCTTCCCAGTACAGGTCCCCGTCAGACCGTGACTCTGCCAGCATTGTT ACAATCGATGCCCATGCACCTCACCATCCAGTGGTGCACCTGTCTGCTGGCAGCACACCCTGGGAGTGGA AGGCTAAAGTAGTGGAGGGTGAGGGTAGCTATGAGCTGGGGGACCTGGCACGCGGCTTCAGAAGCAGCTG TAAACAGCCTGGAATGGGCCCGGGGTCTCCAGTCAGTGATGTGGACCAGGAAGAACCCCGGTTTGGGGCA GTGGCCACTGTCAACCTGGCCACTGGGGAGGGTCTGCCCCCACCAGGTGCAAGTGAAGGGGCCCTAGGTG CAGGCAGCAGAGAATCCACCCTAAGGCGCCAGGTGGGCGGGCTGGCAGAGAGAGAAGTGGAGGCTGAGGC AGAAAGTTATTATAGGAGGATGCAGGCCAGGAGGTACCAGGACTAAGCCTGGCAAAACTGTGATGGGGAT TAGGGGAGCTGGGGGTGTCTACAGCCCCACCAATAAAAACTTATGCTATTAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6808849 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6808849 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7692 same embryo
	EMAGE:7693 same embryo
	EMAGE:7688 same embryo
	EMAGE:7690 same embryo
	EMAGE:7689 same embryo
	EurExpress:euxassay_005299 same experiment
	MGI:4823404 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source