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EMAGE:7702

6430527G18Rik RIKEN cDNA 6430527G18 gene ( MGI:2442463)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7702 EMAGE:7702 EMAGE:7702 EMAGE:7702 EMAGE:7702
"Pseudo-wholemount" of euxassay_007453. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007453_01 euxassay_007453_02 euxassay_007453_03 euxassay_007453_04
EMAGE:7702 EMAGE:7702 EMAGE:7702 EMAGE:7702 EMAGE:7702
euxassay_007453_05 euxassay_007453_06 euxassay_007453_07 euxassay_007453_08 euxassay_007453_09
EMAGE:7702 EMAGE:7702 EMAGE:7702 EMAGE:7702 EMAGE:7702
euxassay_007453_10 euxassay_007453_11 euxassay_007453_12 euxassay_007453_13 euxassay_007453_14
EMAGE:7702 EMAGE:7702 EMAGE:7702 EMAGE:7702 EMAGE:7702
euxassay_007453_15 euxassay_007453_16 euxassay_007453_17 euxassay_007453_18 euxassay_007453_19
EMAGE:7702 EMAGE:7702 EMAGE:7702 EMAGE:7702 EMAGE:7702
euxassay_007453_20 euxassay_007453_21 euxassay_007453_22 euxassay_007453_23 euxassay_007453_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7702Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7702_wholemount_strong.wlz
7702_wholemount_moderate.wlz
7702_wholemount_weak.wlz
7702_wholemount_possible.wlz
7702_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7702_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8966
Entity Detected:6430527G18Rik, RIKEN cDNA 6430527G18 gene ( MGI:2442463)
Sequence:sense strand is shown

>T8966
GGACACCGTAGCCCCCTCCCCCCAGACCCGGAGCTCGCAGCCCTGATCCGCCGCACCAGGAAGAAGGCTC
CCGGTGCTCTTCTCTGGACCAGACAGCCGCAGTAGGAGGTGGGGGCGAGGAGCGTGCGGAACCCGTCGGC
CGCCTGGGTGGTGGAGCCCCCGCCGAGAGAGAGGCACTGCTGCGCCGGCTGTGGAGACTCGCGCTCTCTC
CTGCCCCAGGGGCAGAACTTTCTCGCTCCCCTCCGCCCCCCTCCCCCGCAGCTGGACTTCTTCCCCAGCC
AGCTAGTCCTCCCGTAGGAGAAGGCGCCTCGGAAACAGCCCGGGCGGGGGCCTACCTTCCCCGAGGCTGG
CATCATGTCGGCGGCGCAGGTGTCCTCGTCCCGAAGACAGTCTTGCTACCTGTGCGACCTGCCTCGCATG
CCCTGGGCCATGATCTGGGACTTCTCCGAACCCGTATGCCGTGGTTGCGTCAATTACGAAGGCGCCGACC
GCATAGAATTCGTGATCGAGACAGCACGCCAGCTGAAGCGGGCTCACGGTTGCTTCCAAGACGGTCGCTC
CCCGGGGCCGCCGCCGCCAGTCGGGGTCAAGACGGTGGCCTTGTCCGCCAAGGAAGCGGCAGCAGCAGCT
GCCGCCGCACAGCAACAGCAGCAGCAACAACAGCAGCAACAGCAGCAGCTCAACCACGTCGATGGTTCCA
CCAAACCTGCAGTGTTGGCCGCCCCGTCCGGTCTGGAGCGTTATGGCCTGAGCGCAGCGGCCGCCGCGGC
TGCCGCCGCCGCTGCGGTGGAACAACGCAGCCGGTTCGAGTATCCGCCTCCTCCGGTGAGTCTGGGGAGC
AGCAGCCACGCCGCCCGGCTGCCCAACGGCCTGGGAGGTCCTAACGGCTTCCCCAAGCCGGCACCAGAGG
AAGGCCCCCCGGAGCTGAACCGCCAGAGCCCCAACTCATCCTCAGCAGCAACATCGGTGGCTTCTCGGCG
TGGGACGCATAGTGGGTTGGTGACCGGGCTGCCCAACCCGGGTGGTGGCGGGGGACCTCAGCTAACCGTG
CCCCCCAACCTATTGCCGCAGACGCTGCTTAACGGCCCGGCCAGCGCCGCGGTGCTGCCTCCGCCCCACG
GGTTGGGGGGAAGCCGAGGGCCCCCGACTCCAGCACCTCCGGGTGCTCCCGGGGGCCCCGCATGTCTCGG
GGGTCCCCCGGGTGTGTCAGCCACTGTATCCTCCGCTCCATCATCGACTTCGTCGACGGTGGCAGAGGTG
GGCGTGGGTGCTGCTGGCAAGAGGCCTGGATCGGTGTCGAGCACGGACCAGGAGCGTGAGCTAAAGGAGA
AGCAGCGCAACGCTGAGGCCTTGGCAGAGCTGAGCGAGAGCCTGCGCAACCGCGCGGAGGAGTGGGCCAA
CAAGCCCAAGATGGTCCGCGACACGCTGCTCACGCTGGCTGGCTGCACGCCCTACGAGGTGCGCTTCAAG
AAGGACCACTCGCTTTTGGGTCGCGTCTTTGCCTTCGACGCGGTCTCTAAGCCGGGCATGGACTATGAGC
TGAAGCTGTTCATTGAGTATCCCACGGGCTCCGGCAATGTGTACTCCAGCGCGTCTGGGGTGGCCAAACA
GATGTACCAGGATTGCATGAAGGACTTTGGCCGGGGTCTGTCCTCTGGCTTCAAGTACCTGGAGTACGAG
AAGAAGCACGGCTCTGGCGACTGGCGCCTACTTGGGGACCTACTGCCTGAGGCTGTGCGCTTCTTCAAGG
AGGGCGTGCCCGGCGCGGACATGCTGCCCCAGCCCTACCTAGATGCTAGCTGTCCCATGCTACCCACGGC
GCTGGTGAGTCTCAGCCGCGCCCCCAGCGCACCTCCAGGAACTGGAGCCTTGCCACCCGCGGCGCCCACG
GGCCGGGGTGCAGCGTCCAGCCTGCGCAAAAGGAAGGCATCCCCGGAGCCTCCAGACTCAGCTGAAAGCG
CACTGAAGCTCGGTGAGGAACAGCAGAGGCAGCAGTGGATGGCAAACCAGAGCGAGGCGCTGAAGCTCAC
GATGTCCGCGGGGGGCTTTGCGGCGCCGGGACACTCAGCAGGGGGACCACCTCCGCCCCCTCCACCTCTG
GGACCTCATTCCAATCGGACCACCCCGCCGGAGTCAGCCCCCCAGAACGGTCCGTCCCCCATGGCTGCCC
TCATGTCGGTAGCAGACACTTTGGGCACAGCTCATTCGCCCAAGGACGGCAGTTCAGTGCACTCGACCAC
TGCATCTGCTAGGCGAAACAGCAGCAGCCCGGTGTCACCCGCCTCCGTGCCGGGCCAGCGTCGCCTGGCA
TCACGCAATGGGGACCTGAATTTACAGGTGGCGCCCCCGCCGCCTAGCGCCCACCCGGGCATGGACCAAG
TACACCCCCAAAACATTCCGGATTCCCCTATGGCCAACAGCGGACCCCTCTGCTGTACCATTTGCCACGA
ACGTTTGGAGGACACGCATTTCGTTCAGTGTCCGTCTGTCCCTAGCCACAAATTTTGTTTCCCTTGTTCT
AGAGAGAGTATCAAGGCCCAGGGGGCCACAGGTGAGGTGTATTGCCCCAGCGGAGAGAAATGCCCCCTAG
TCGGGTCTAATGTACCTTGGGCCTTCATGCAGGGCGAAATTGCAACTATCTTAGCTGGGGATGTTAAAGT
GAAAAAAGAGAGAGACCCTTGAACCGCAGGGCAGCGACCTCCTTTGCCCTAGACCACCTCCTCTTCAATC
TAGAGGGCCCTTCCCCCACCTGCCTCCACCTCCCCTATCCACCAAGATGTAGAATTGTGAATATAACTGC
AAAAAGTTAGTCTTATGTATAGACATTATTTTCGTCGTATGTTTCTATATTTTGAAACAGGTATGTAACT
TCTTCATTTGAAGGATAAGCTGGTTTGTGTTAAGCAGTATAATATTGATTGGGTCTTTCGCATCATATTC
GTTAGCATTTATTTGGTGGCAGAATGTTTGCCTAGGTACAGAATTAATAGCCCTTAGCAACGACTGCTGC
TGGTGTGTATTTTTGTAAATGTTATGCACTCTGAAAGGAAAACACACAAAAGAAGAGATTTTGTCGTTTT
TTGTTTTGCCAAGGCCAGTGTTGCTGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6810092 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810092 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7704 same embryo
 EMAGE:7703 same embryo
 EMAGE:7705 same embryo
 EMAGE:7701 same embryo
 EMAGE:7700 same embryo
 EurExpress:euxassay_007453 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS