EMAGE mouse gene expression spatial database

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EMAGE:7704

Hey1 hairy/enhancer-of-split related with YRPW motif 1 ( MGI:1341800)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005307. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005307_01	euxassay_005307_02	euxassay_005307_03	euxassay_005307_04

euxassay_005307_05	euxassay_005307_06	euxassay_005307_07	euxassay_005307_08	euxassay_005307_09

euxassay_005307_10	euxassay_005307_11	euxassay_005307_12	euxassay_005307_13	euxassay_005307_14

euxassay_005307_15	euxassay_005307_16	euxassay_005307_17	euxassay_005307_18	euxassay_005307_19

euxassay_005307_20	euxassay_005307_21	euxassay_005307_22	euxassay_005307_23	euxassay_005307_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7704_wholemount_strong.wlz

7704_wholemount_moderate.wlz

7704_wholemount_weak.wlz

7704_wholemount_possible.wlz

7704_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7704_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

pituitary gland

moderate

regional

moderate expression: see section 11 12 16

telencephalon ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24 weak expression: see section 03 04 05 06 07

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 12 14 15

mandible

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 18 19 20 21 22 23 weak expression: see section 12 15 16 17

maxilla

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20 weak expression: see section 12 15 16 17

kidney calyx

moderate

regional

moderate expression: see section 07 09 10 17 18 19 20 weak expression: see section 08

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8964
Entity Detected:	Hey1, hairy/enhancer-of-split related with YRPW motif 1 ( MGI:1341800)
Sequence:	sense strand is shown >T8964 AGCGTGGGAAAGGGATGGTTGAGTTTTAACCGGAGACTGAGCGTGAGTGGGATCAGTGTGCACGCACCTC CCGCAGCCGAGCGCTGAGCGGCCACTGCAGTTAACTCCTCCTTGCCCGCCGCGCGACCCTCCTCGGAGCC CACGCTCCGCCACCATGAAGAGAGCTCACCCAGACTACAGCTCCTCAGATAGTGAGCTGGACGAGACCAT CGAGGTGGAAAAGGAGAGCGCGGACGAGAATGGAAACTTGAGTTCGGCGCTGTGTTCCATGTCCCCAACG ACATCGTCCCAGGTTTTGGCCAGGAAAAGACGGAGAGGCATCATCGAGAAGCGCCGACGAGACCGAATCA ATAACAGTTTGTCTGAGCTGAGAAGGCTGGTACCCAGTGCCTTTGAGAAGCAGGGATCTGCTAAGCTAGA AAAAGCTGAGATCTTGCAGATGACTGTGGATCACCTGAAAATGCTGCACACTGCAGGAGGGAAAGGTTAT TTTGACGCGCACGCCCTGGCTATGGACTATCGGAGTTTGGGGTTTCGGGAATGCCTGGCCGAAGTTGCCC GTTATCTGAGCATCATTGAAGGACTCGATGCCTCCGACCCGCTTCGCGTTCGCCTGGTCTCCCATCTCAA CAACTACGCATCCCAGCGGGAAGCCGCGAGCGGCGCTCACGGTGGCCTCGGACACATTCCCTGGGGAAGT GCCTTCGGACATCACCCACACATCGCACACCCTCTGCTGCTGCCCCAGAATGGCCACGGGAACGCTGGCA CCGCGGCGTCACCCACGGAGCCGCATCACCAGGGCAGGCTGGCTTCTGCCCATCCGGAGGCGCCGGCCTT GCGAGCGCCCCCTAGTGGCGGCTTGGGACCGGTGCTTCCCGTGGTGACCTCGGCCTCCAAACTGTCTCCA CCGCTGCTCTCCTCTGTGGCCTCGCTCTCAGCCTTCCCCTTTTCCTTCAGCTCCTTCCACCTACTGAGCC CTTCGACACCCACGCAGGCAGCAAACCTTGGCAAGCCCTATAGACCTTGGGGGACAGAGATCGGAGCTTT CTAAAGAACTGATGCTGTAGAACAAGGGAGGGGAAAGCTTAAAATCCCAGGTGTGTTGGGACGGTTGTCA ACACCACCCTAAAGTCGCCAGTAAGTCAGGAAAAAGGTACATTTTCAGATAATTTTTTTCTAAAGACTAA AAGTTTGTTGGTTTACTTTTCTCTTTAATTTTTTATCATGTCATGCATTAGCAGTTTTAAAAAATTAGTT GTTAATTTTTGTTTAAAAGATTCAATTGAGGTAGTGATTACGAACCAACACTTTGATCCGTTGTTTGTTC TGTGCCTCATTTATTTTGTAAACCTGTCTGTCTGAGAATGATTCCGTTTGCCTCAGCGTGGGGAATCTTA ACATTAGTGTTTGGGGTCTGTTTCCTGGTGTGTATAAGTTGTAATCTTTTGAGGATTAATTTCGCACGCC ACTATGCTCAATGTTAACACGATTTTGTTACTACTTTGATGGACCGAGGTGTTGTATAAGTGGTATTCTT TGGGGAGGGAGGGTCAGCAAAGCATTATATTTGCAAACAAAGCGTTGACAAATCAGATGCGCAGCTTTAC TGGAGAGCACTGGCTCTCTCTCTGCCTCTCAGAGCAGTGAGGTGAAGGGAGAAAGGTGTCTGTGCCCTGA ATCCAGATGACCAGCTACTGTGGCCTGCTTGGCTTTTCTCTTCTGATATTTCAGGTTTAGAAACAGCTGC TGGTAGTCTAGGTCCCCATTTGGAGCGTTGGCAGCAAGCAAGACAGTTATGTAGACCTTGCCTGCACTTG GCAGCCCTAAGCACTCTCAGTCTCACGGATTTCACCGTTCACCAGTGTCGACCTGCGTAAGCGATCGGAG TCTGAAAGTAGCTTGGTGCCTGTGAAACACAACCCGATTTTCCTAGAACTCCCATATTTTCTTTTAAGTG GAAATTTTTATGTTGTGTTTTCCTTTTGGTGCATGAAAATGTGGTTCTTGCAGTACTTAAAAGGGCTTCT CTGCCTTCTCATTCATTTTTAAAATTTTGATTTGGGCTCTAAAAGTATTGTTTTACAGGCTTACCCCTTT AGAAGGTATAATTTGAACAGCTCCTCTGAACTAGGTTTGACCTCTGTTGTATTGATGTGTTGTGACTAAA TAAAAAGCAAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6809680 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6809680 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7703 same embryo
	EMAGE:7702 same embryo
	EMAGE:7705 same embryo
	EMAGE:7701 same embryo
	EMAGE:7700 same embryo
	EurExpress:euxassay_005307 same experiment
	MGI:4825364 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source