EMAGE mouse gene expression spatial database

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EMAGE:7705

Abcd1 ATP-binding cassette, sub-family D (ALD), member 1 ( MGI:1349215)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007455. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007455_01	euxassay_007455_02	euxassay_007455_03	euxassay_007455_04

euxassay_007455_05	euxassay_007455_06	euxassay_007455_07	euxassay_007455_08	euxassay_007455_09

euxassay_007455_10	euxassay_007455_11	euxassay_007455_12	euxassay_007455_13	euxassay_007455_14

euxassay_007455_15	euxassay_007455_16	euxassay_007455_17	euxassay_007455_18	euxassay_007455_19

euxassay_007455_20	euxassay_007455_21	euxassay_007455_22	euxassay_007455_23	euxassay_007455_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7705_wholemount_strong.wlz

7705_wholemount_moderate.wlz

7705_wholemount_weak.wlz

7705_wholemount_possible.wlz

7705_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7705_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8965
Entity Detected:	Abcd1, ATP-binding cassette, sub-family D (ALD), member 1 ( MGI:1349215)
Sequence:	sense strand is shown >T8965 GAAGGAAAGACACAGCCTCTCTCCTTAAGACCTCTCGAGGCTCTGACCTCAGGGGCTAAGGGGACAGTCT GTAGAGGGAAGCCAGTTCCTCCACCCGGGCTGCCTGAAGAGGCCACGACCCTGGAGGATTCTAACCCAAC CGATCCAGGGGCCCCAGCACCACCGCGAGGGCCTAAAGCAACAGTCTCAGGGATCACTTCAAGGTTTCCA GTTGCCTAGACAACGAGCCCTGGGTCAGAGCAACAACCCTTCCAGGATCTGTCTGCAACTGTAGCCTCAG GCAGCAGTTCCAGCCCTCAGCCTGCTCACCAGTCCAGGTGACATGCCGGTGCTCTCCACTCCCCGGCCCT CACGGGTGACCACGCTGAAGCGCACAGCTGTGGTCCTGGCCCTCACAGCCTATGGAGTCCACAAAATCTA CCCTCTAGTACGGCAGTGTCTGACTCCTGCCAGAGGTCCTCAGGTGCCAGCTGGGGAGCCCACTCAAGAG GCCTCTGGAGCCACCGCAACCAAGGCTGGCATGAACCGGGTATTCCTGCAGCGGCTCTTGGCGCTCCTGA GGCTGCTGTTCCCCCGAGTCTTGTGCCGGGAAACAGGGCTGCTGGCCCTGCATTCTGCTGCCCTGGTAAG CCGAACTTTCCTGTCTGTGTATGTTGCCCGCCTGGACGGCAGACTGGCCCGCTGCATTGTACGTAAGGAC CCGCGGGCCTTTAGCTGGCAACTGCTGCAGTGGCTCCTCATCGCCCTTCCTGCCACTTTCATCAACAGTG CCATCCGCTACCTAGAGGGCCAGCTGGCTCTTTCTTTCCGAAGCCGTCTAGTAGCTCATGCCTATGGACT CTACTTCTCCCAACAGACTTACTACCGAGTAAGCAACATGGATGGACGACTTCGAAACCCTGATCAGTCT CTGACAGAGGATGTGGTAGCCTTTGCTGCCTCTGTAGCCCACCTTTATTCCAACCTGACCAAGCCACTCC TGGATGTGGCTGTGACCTCCTACACTCTCCTTCGAGCTGCCCGATCCCGAGGAGCTGGCACAGCCTGGCC CTCAGCCATCGCTGGCCTGGTGGTGTTCCTCACAGCCAACGTGCTTCGAGCCTTCTCTCCCAAGTTTGGG GAGCTAGTGGCAGAGGAGGCACGGCGAAAGGGGGAACTGCGCTACATGCACTCTCGAGTGGTGGCCAACT CAGAGGAAATTGCCTTCTACGGGGGCCATGAGGTGGAACTGGCACTACTACAGCACTCCTATCAAGACCT AGCTTCACAGATCAACCTCATCCTGCTTGAGCGCCTATGGTATGTCATGCTGGAACAGTTCCTCATGAAA TATGTGTGGAGTGCATCTGGCCTGCTCATGGTAGCTGTCCCCATCATCACAGCCACTGGCTATGCAGAGT CAGACTCAGAAGCCATGAAGAAGGCAGCCTTGGAAATGAAGGAAGAGGAGCTGGTTAGTGAGCGTACAGA AGCTTTCACCATTGCCCGAAACCTCCTCACAGCTGCTGCAGATGCCACCGAAAGGATCATGTCATCTTAT AAGGAGGTGACAGAGCTGGCTGGCTACACAGCCAGGGTGTACGAGATGTTCCAGGTATTTGAAGATGTCA AACACTGTCGTTTTAAGAGGACAGGAGATCTAGAGGAGGCTCAGGCTGGGCCTGGGGTCATGGTACAGTC TGGTGTCCATGTAGAGGGGCCCCTGAAGATCCAAGGCCAAGTTGTGGATGTGGAGCAGGGGATCATCTGT GAGAACATCCCTATCATCACACCCACTGGAGAGGTGGTGGTGGCCAGCCTCAACATCAGGGTGGAGGAAG GCATGCACCTGCTCATCACAGGCCCCAATGGCTGTGGCAAGAGTTCTCTGTTCCGAATCCTAGGTGGACT CTGGCCCACATACAGTGGTGTACTCTATAAGCCCCCACCCCAGCGCATGTTCTATATCCCTCAGAGGCCC TACATGTCCGTGGGCTCCTTGCGTGACCAAGTGATCTATCCCGACTCTGCGGAAGACATGCGGAGGAAGG GCTGCTCGGAGCAGCAGCTGGAAGCAATCCTGGGCATCGTGCATCTCCGCCACATCCTGCAACGGGAGGG AGGTTGGGAGGCAGTGTGTGACTGGAAGGACGTCCTGTCTGGAGGTGAGAAGCAGAGGATTGGTATGGCG CGCATGTTCTACCACAGGCCCAAGTACGCCCTCCTGGATGAGTGCACTAGTGCCGTGAGCATTGATGTGG AAGGCAAGATCTTCCAAGCAGCCAAAGATGCTGGTATCGCACTGCTCTCCATCACCCATCGACCCTCCCT ATGGAAGTACCACACACACTTGCTGCAGTTTGATGGGGAAGGAGGCTGGAAGTTTGAGAAGCTGGATTCT GCTGCCCGCCTGAGCCTGACTGAAGAGAAGCAGCGCCTGGAGCAGCAACTGGCGGGCATCCCCAAGATGC AGGGGCGCCTTCAGGAGCTCCGCCAGATCCTTGGCGAAGCTGCAGCTCCAGTTCAACCCCTGGTCCCAGG AGTCCCCACTTGACTGGGGACCTTATCCCCAGCTCCCACCCTTCTCCAGGCTCTCGGATCACATGAAGGA AGCTCCTCCCCTCCCCCATTGCCCTGCATGCCTTACCCCTCCCAAGCCTCCTAGCCAATTACCTGCATTC CCTGAAGACCCCACTCTGGGACAGGTAGATATGAATGGGGACACACTGCATTCCACCTTCACTCTGGGTT CTGCTCCAGGAACTCTGCCCTGTTCAGGGCAGTGTGTCTTTTACTTTTCCCTGGGGAGGGATGGAGGGTT TGGAAGTCCCCAGTCCTGACTCTATACCAGTATGGCTGAGCCATAGGAAGTAAGCAGGAGCTGCCTCCCT TACTGCCAGCAGCCAGAATGTGTGGCAACCCTGGTAGTTGTGGGTTACATCCCTGCCGAAACCCAAATCC TTGTCATTGTTTTGATCCATCCCTTTGTTGCCATCCCCTTGTGTGACCAGCCAATGCCCCTGTCCCACAG GATACCCCCTGCCATGAAAACAGCCTAATGGGGCCCAGGATTAGACAGGGCCCAAAGTGTGCCCTTCCCC ATGGTTACCCAAGAAGGACAGAGTTCCTCCTGAGCCATGAAGAGAATATAGCCCTGGCCCTCCCACTCCT CCACCTCTACAACCTAATTTATTGGACTCCCAGTTTATAGTTGTCTAAATTGCCAACATGAGTACCCTGC CAGCACAGGAGGTGACTGGGTGGTCCCTTCAATCTACCCAGTCTCTGGTGCCAAACCTGGCCCTTCACCA GCCTATACTGCCAGTTGCCACTGGGGGTGCCCTCTGCCAGCTAGGCAGAAGTGAGGGCACCATGTTCCCA GCTCAGTGCCAAAGAGGGGTCACAGGAGGTGCTGTGTCTGCTAAACCAGCCCTCACCAAGAGGGAGACGC CCCCACACACACACCCTACTGCCATTGTGTGCCTTTCCTGGGCCTTCAGCCCTCCAGCTGGGGTACCCCC CTCAGTCCTCTCAGTAAAAACCTCTGGAAAGTACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810147 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810147 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7704 same embryo
	EMAGE:7703 same embryo
	EMAGE:7702 same embryo
	EMAGE:7701 same embryo
	EMAGE:7700 same embryo
	EurExpress:euxassay_007455 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source