EMAGE mouse gene expression spatial database

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EMAGE:7717

Mgrn1 mahogunin, ring finger 1 ( MGI:2447670)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007472. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007472_01	euxassay_007472_02	euxassay_007472_03	euxassay_007472_04

euxassay_007472_05	euxassay_007472_06	euxassay_007472_07	euxassay_007472_08	euxassay_007472_09

euxassay_007472_10	euxassay_007472_11	euxassay_007472_12	euxassay_007472_13	euxassay_007472_14

euxassay_007472_15	euxassay_007472_16	euxassay_007472_17	euxassay_007472_18	euxassay_007472_19

euxassay_007472_20	euxassay_007472_21	euxassay_007472_22	euxassay_007472_23	euxassay_007472_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7717_wholemount_strong.wlz

7717_wholemount_moderate.wlz

7717_wholemount_weak.wlz

7717_wholemount_possible.wlz

7717_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7717_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8995
Entity Detected:	Mgrn1, mahogunin, ring finger 1 ( MGI:2447670)
Sequence:	sense strand is shown >T8995 GGAGCGGCCGCAGCGGCGGCGGCGGCGGTGGCGGCGGTGGGTACGCGTTGGTGCGGCCTGTTTTGGGCCA TGTCCGTGTGAGGACGCCGCAGCCGACAACCTCTCCTCTCCGGCTGGCCGGTAGAAACGCGCACCATGGG CTCCATCCTGAGTCGCCGCATCGCGGGCGTAGAGGACATCGACATACAGGCCAACTCGGCCTATCGCTAC CCTCCGAAGTCCGGAAACTACTTTGCTTCGCATTTTTTCATGGGAGGAGAGAAATTTGACACTCCCCATC CTGAAGGTTACCTCTTTGGAGAGAACATGGACCTGAACTTCCTGGGCAGTCGTCCAGTCCAGTTTCCCTA TGTCACCCCTGCCCCCCATGAGCCAGTGAAGACACTGAGGAGCCTGGTGAACATTCGCAAAGACTCTCTC CGGCTTGTGAGGTACAAAGAAGACGCAGACAGCCCCACGGAGGACGGTGAAAAGCCCCGCGTGCTCTACA GTCTGGAATTCACCTTTGATGCCGATGCCCGTGTGGCTATCACCATCTATTGTCAGGCGGTAGAGGAGCT GGTGAATGGCGTGGCAGTGTACAGCTGTAAGAACCCTTCTCTGCAATCTGAGACCGTCCACTACAAGAGA GGCGTTAGCCAGCAGTTCTCCCTGCCTTCTTTCAAGATTGACTTCTCCGAGTGGAAGGATGATGAGCTGA ACTTTGACCTGGACCGGGGTGTGTTTCCAGTAGTCATCCAGGCTGTGGTGGACGAAGGGGATGTTGTGGA AGTGACTGGCCACGCTCATGTGCTCCTGGCTGCCTTTGAGAAGCATGTGGATGGCAGCTTCTCCGTGAAG CCATTAAAGCAGAAGCAAATTGTGGACCGGGTCAGCTACCTGCTGCAGGAGATCTACGGCATCGAGAACA AGAACAACCAGGAGACTAAGCCTTCTGATGATGAGAACAGTGACAACAGCAGTGAGTGTGTGGTGTGCCT GTCAGACCTGCGGGACACACTGATCCTGCCCTGCCGACACCTGTGCCTCTGCACTTCCTGTGCTGACACA CTGCGGTACCAGGCCAACAACTGCCCCATCTGCCGGCTGCCGTTCCGAGCCCTTCTGCAGATCCGGGCTG TGCGGAAGAAGCCAGGGGCCCTGTCTCCCATCTCCTTCAGCCCTGTCTTGGCCCAAAGTGTGGACCACGA TGAGCACTCTAGTTCTGACAGTATCCCACCTGGCTATGAGCCCATCTCCTTGCTTGAGGCACTCAATGGT CTACGAGCTGTCTCCCCGGCTATCCCATCAGCTCCCCTCTATGAGGAAATCACCTACTCAGGCATCTCGG ACGGTCTTTCCCAGGCCAGTTGTCCCCTTGCTGGACTTGATCGAATCATGGAAAGTGGCCTACAGAAGGG CAAGACACAGAGCAAGTCTCCAGACAGCACCCTGCGGTCTCCATCATTCCCCATCCATGAGGAGGACGAG GAGAAGCTCTCAGAGGACTCGGATGCTCCTCTCCCACCGAGCGGTGTGGAGCTGGTACTGCGGGAGAGCA GCTCCCCTGAGAGTTTCGGAACAGAGGAGGGAGATGAGCCGTCCCTAAAGCAAGGGAGCCGAGTACCCTC TATTGATGATGTCCTGCAAGATGGCAGTCCGCAGCATCATGGTTGCAGCCAGCCAGTCCCTCCTGCTGAC ATCTACCTACCAGCCCTGGGTCCTGAGTCGTGCTCTGTTGGTATAGAGGAGTAAGCTGGATGGTCCACCT CCATGGAGACGCCACACAGCCTTGGCACCACCAGCTCCCCCTGGCCTCTGCTGAGTGGCTCTAGTCCTGA GCCTGGAGTTGCTGAGCTGACCCCATTCTGAAAGTCTGGCTTAGCGGGCAACACAGAGCCCTCAGCATCC CAAACATTGCCAGGAGGCTTCCCTGGACCCCAGCGAGACCCTGGCCTCTCTTGTCTGCACGCCTCATGTG GCCACTGGGATTTGAGGGTCCACAGTGAACCTTGATCAAATTGCACAGTGGGTTGTTCCTCCACACACCC TCCTTTTTTTACGGTCAATATATTTGTAAATGGTACAAGATGAAGGACAACAACTGTCTCCATGGGCCAC GTGCCCTAGAAGGACTCTCCCCAACTGTCAAAGCTAATGCTTTGAGCACTTAGCAAACCAGCATAAGAGA ATCTACCCATGGAATGTTCTGGAAGGTGGGCAGTCCAGGTTACAGTTGATGAAGGAAACAGTGCCTGTCT GCCTCTCATGTGTGACCCCAAGGCAGCCAGGCTGTCACTGCTGCTGTCACAGGCTGACACCTTACAGATG TCCTGACCATGGGTGACCCTCCTCGGAGCGTCCAGATCCCCTCCTGCTCTGCTCTATTACCTACTAGTGG TCCCTGGCAGCTGTGGGCAAGGCCTGGGTAGTCAGACTGTTAACACCACAGTCCTTAGCAGTGGTCAATG TCCAGTTCCCCCGCACAGGTCCTGCTTCACTCACATCCTCACCCGCTGCCAAGGTGGCGTTTTGTGCTTG CAGGGCAGCACTGCCCACCACCTGCCAGGGAGGAGCCTGAGCAGCTTCCATAGCAGATCCGTTGCCAGTT ACCTGGCCTGTAAGCTTCTTACTGATTGATTGTCTAAGGCTCCTGTCAGAGCCCACCTAGAAGCAGGCTC CTAGTGATACTTTGTTTCCAAGGCAGAAGTGGCCCATGAGTGGCCCCAGAGCAGAGGTGCCCCAGGCAGT GCAGCTCCCTGGTGGGTTGGTAGAGGGACCTCAGCTTAAGGAACTGCCCTGCCACTCACCCAGGTGACAG CCTGTAGCAGTGCATGGAGACCCCTTTAGGCTCAGGGCTGCTCGAGCTCTGACTCTAACCCTGCTCCTTC CTAGCACAGCTCTGCACATCCATCTCTCTCTCTTGCTGAATGCACATATGTGCACACATGTCAGCATGTG CATGCTCACTCCCCCACTTCTCACCTGCCAACATCCTGGCCTCTGGCCGTGGCACTGGTGCCAGTACTTT GAAAGATGGGGTGCAGCCTACACACTTACCTGTCTCAGACTCACATGTACTATCATCTGATGTACTCTGA TTGGCTAGTTTGGGTTCATTTTTACTGTATATTTATAGTAATAAAATCATGCAGCAATAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6811606 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6811606 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7718 same embryo
	EMAGE:7719 same embryo
	EurExpress:euxassay_007472 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source