EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7728

Kcnj11 potassium inwardly rectifying channel, subfamily J, member 11 ( MGI:107501)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007498. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007498_01	euxassay_007498_02	euxassay_007498_03	euxassay_007498_04

euxassay_007498_05	euxassay_007498_06	euxassay_007498_07	euxassay_007498_08	euxassay_007498_09

euxassay_007498_10	euxassay_007498_11	euxassay_007498_12	euxassay_007498_13	euxassay_007498_14

euxassay_007498_15	euxassay_007498_16	euxassay_007498_17	euxassay_007498_18	euxassay_007498_19

euxassay_007498_20	euxassay_007498_21	euxassay_007498_22	euxassay_007498_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7728_wholemount_strong.wlz

7728_wholemount_moderate.wlz

7728_wholemount_weak.wlz

7728_wholemount_possible.wlz

7728_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7728_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

weak

regional

weak expression: see section 08 09 10 12 14

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 13 14 15 16 17 18 19 20 21 weak expression: see section 10 12 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9048
Entity Detected:	Kcnj11, potassium inwardly rectifying channel, subfamily J, member 11 ( MGI:107501)
Sequence:	sense strand is shown >T9048 CCCGGAGGTGACAAGTTCTGAGGTGGTTTGAGAAGAAAGGCTGGGCCAGGGAAAGGGAGTTAGAGGGGGG TCCTGGGGCGGTGGAGGAGAGCTGAGCGGGTAGAGCACAAGCTGGGTTGGGGGCTCAGTAAGCAATGAGC AGGTGGAGCCGGCATCTGAGGCTAGTAGTATAAAGAGAAGCAGAACCCAGCTGAGGGGCATACCAGCCTA GAGGTTGGTACAAGCTTAGGGTAACCTGAGGAGAGGGCTCAGAGAGGGGGACCCCACAAAGAGGCCCTAG GCCAAGCCAGTGTAGTGCCTCCCCCCATGGGGGAAACCCCTTCCCGGGGCCAACGGAGCCATGCTGTCCC GAAAGGGCATTATCCCTGAGGAATATGTGCTGACCCGGCTGGCAGAGGACCCTGCAGAGCCCAGGTACCG TACTCGAGAGAGGAGGGCCCGCTTCGTGTCCAAGAAAGGCAACTGCAACGTCGCCCACAAGAACATTCGA GAGCAGGGCCGCTTCCTGCAGGATGTGTTCACCACGCTGGTGGACCTCAAATGGCCACACACTCTGCTCA TTTTCACCATGTCCTTCCTGTGCAGCTGGCTGCTCTTTGCCATGGTCTGGTGGCTCATCGCCTTCGCCCA CGGTGACCTGGCCCCCGGAGAGGGCACCAATGTGCCCTGCGTCACAAGCATCCACTCCTTTTCATCTGCC TTCCTTTTCTCCATCGAGGTCCAGGTGACCATTGGTTTCGGCGGGCGCATGGTGACAGAGGAATGTCCCC TGGCCATCCTCATTCTCATTGTGCAGAATATCGTCGGGCTGATGATCAACGCCATCATGCTGGGCTGCAT CTTCATGAAAACGGCCCAGGCCCATCGGCGGGCAGAAACCCTCATCTTCAGCAAGCATGCTGTGATCACC CTGCGCCATGGCCGCCTGTGCTTCATGCTGCGCGTAGGGGACCTCCGAAAGAGCATGATCATTAGCGCCA CCATCCACATGCAGGTGGTGCGCAAGACCACCAGCCCCGAGGGCGAAGTTGTGCCTCTCCACCAGGTAGA CATCCCCATGGAGAATGGCGTGGGTGGTAACGGCATCTTCCTGGTGGCCCCACTCATCATCTACCACGTC ATCGACTCCAACAGCCCGCTCTACGACCTGGCTCCTAGTGACCTGCACCACCACCAGGACCTGGAGATCA TTGTCATCTTGGAAGGCGTGGTAGAAACCACGGGCATCACCACCCAGGCCCGCACCTCCTACCTAGCTGA CGAGATTCTATGGGGGCAGCGCTTTGTCCCCATTGTGGCCGAGGAGGACGGCCGCTATTCTGTGGACTAC TCCAAATTTGGTAACACCATTAAAGTGCCCACACCACTCTGCACAGCCCGCCAGCTTGATGAGGACCGCA GTCTGCTGGATGCCCTGACCCTCGCCTCGTCGCGGGGGCCCCTGCGCAAGCGCAGTGTGGCTGTGGCGAA GGCCAAGCCCAAGTTTAGCATCTCTCCAGATTCCTTGTCCTGAGTTGCAGTTCCTCAGGCCCCCACTCAC TTGTGTGGGCACGTGGAAAGTGAAGTATGGTATGTAGAGTGGTGGGGTGCGAGCCTCTTGGCCAGACGAG GGTCTGGTGTGGGACAAGACCCTGCTCGGCTCAGCCTCCCCGCTGCTGTGTGTCTGGGGTGTTACAAGAT ACTTGTCACTATGCTATTTCTGGCCCCAGCAGGAGCCTGTACCGGGTTATTTTTGTTCCTGCTCCTCCCA GGCTCAGCTCGGGACTGGTTTACCCCTGTGTCCCTGCCCAACGGTGGGGAGACCACAGAAGGTTCAGGGC CTCTAAAGCTAAGGTCTCAAAGCCCGTCTTGGCCCTCCATGATTCATCTTGCCACAAGCAGACAGGTAAT TGGGCAAAAGCAACCCCCCAAGTTGGCAGCCTATTGTTGTTTCTGTAGCCACTAAGAAATACCTGGCCAG GCCCCAGCCCTGTTCGTGCCATGCTGAAGTATCCTGCCAGCCAGGGAGGAAGGTGGGTGTCAGAGCATGG GAGCTGAGGAGCCCACACAGACACGGCGGGATAAGTCTACCTAGGCTCACCTCCATAAGGCAGGCTTGTG TGAGGCAGGGGGACAAGAAGGGGGCAAATGATTGGAGACCTTTCTAGTCCTGTGCAGACATGTGTCCCTG TCCTCTAGCCTTGACTCAGCCTTCTGCTGACAGTCAGGAGTGCTGCCTCATTTTGTCGTCGTTCCCCCCC CCCCCCAGAGGCTGGGGAGTGGAGTGTTGGGCAAACACTGATATACCTGGATGAGAGGCCAAAAGGGGTC CTGTGCTTGCCATACAAGATGCACTTCAGGCAAAACTCTGCTTTCAGCTCCCATGGGCAGCTGTGAGTCA CTTTGTCTGGGGGACGCTGGTGGGGTAGCGGTGCCTTGTCTGGGTTACTAGAAAAAGAAATATTGGGAAG TTCAGCCTGGTGAGGGAAAAGGAAGTCCCCAAGGCCCTTGGGCATGACCTGAGCCTGGACTCTCAGAGCA GTGTGGTGTCCTTGCCCAGAACTCCACTGCCCCATTGTCATAAAGCCTCCCACCTGTGACCTGAACTCTG GGCGTTTCTTGCTGGGGAGGCAGTGGGCATGCCACTGGAAAAAAATAAAGGGCACTGCCAGAGTCTGCCA CCCAACATGGCCCTGACAGGCAAGAGGCCAGGAAAGCTACTTAGACCAGCTCTGTTTCCCTCATCTCTGG AAGCCACCATCCTTCCACCTCAGGTCCCCCCACCCCCACCCATTCTCTGTCTGTCTGGCCAACTTGGCCA TTTGACATCCACCAGACATCGTAGTCAAGGGCCTGGCCTCCCTCCCGAGACTCTGCTGGAAGGCTGAGGT AGCCATGGGGAGGGAAGAGAGACGTTGTAAGCTTTAGGGCACTGATTGGTTCAAATCCTGGCTCCGGCCG AACAGCTCTGTGACTCAGTTGTCTCATTCATGTTAATAGTCCCACCTCATTAAGCTGAGCAGGTGAAAGC ACATGTATCCATGCTTCAGAAGTGCCTGGTCTATAACAAGTACTCAATAAATGTTAACTGTCGTGAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6816299 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6816299 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7726 same embryo
	EMAGE:7730 same embryo
	EMAGE:7727 same embryo
	EMAGE:7729 same embryo
	EurExpress:euxassay_007498 same experiment
	MGI:4825715 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source