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EMAGE:7731

Gabbr1 gamma-aminobutyric acid (GABA) B receptor, 1 ( MGI:1860139)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7731 EMAGE:7731 EMAGE:7731 EMAGE:7731 EMAGE:7731
"Pseudo-wholemount" of euxassay_009799. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009799_01 euxassay_009799_02 euxassay_009799_03 euxassay_009799_04
EMAGE:7731 EMAGE:7731 EMAGE:7731 EMAGE:7731 EMAGE:7731
euxassay_009799_05 euxassay_009799_06 euxassay_009799_07 euxassay_009799_08 euxassay_009799_09
EMAGE:7731 EMAGE:7731 EMAGE:7731 EMAGE:7731 EMAGE:7731
euxassay_009799_10 euxassay_009799_11 euxassay_009799_12 euxassay_009799_13 euxassay_009799_14
EMAGE:7731 EMAGE:7731 EMAGE:7731 EMAGE:7731 EMAGE:7731
euxassay_009799_15 euxassay_009799_16 euxassay_009799_17 euxassay_009799_18 euxassay_009799_19
EMAGE:7731 EMAGE:7731 EMAGE:7731 EMAGE:7731 EMAGE:7731
euxassay_009799_20 euxassay_009799_21 euxassay_009799_22 euxassay_009799_23 euxassay_009799_24
EMAGE:7731
euxassay_009799_25

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7731Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7731_wholemount_strong.wlz
7731_wholemount_moderate.wlz
7731_wholemount_weak.wlz
7731_wholemount_possible.wlz
7731_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7731_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25
facial vii ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 18 19 20 21 22
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 06 07 18 19 20
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22
vagus x ganglion
strong strong
regionalstrong expression: see section 08 18 19
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 06 07 08 17 18 19 20
trigeminal v nerve
strong strong
regionalstrong expression: see section 09 10 17
spinal cord
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 10 16 17 moderate expression: see section 11
cervical ganglion
strong strong
regionalstrong expression: see section 09 17 18
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 12 13 14 15
dorsal root ganglion
strong strong
regionalstrong expression: see section 09 10 11 14 15 16 17 18 19 moderate expression: see section 08
neural retina
strong strong
regionalstrong expression: see section 01 02 03 23 24 25
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 17 18 19 moderate expression: see section 08 10 11 weak expression: see section 12 14 15 16
tongue
strong strong
spottedstrong expression: see section 13 15 17
stomach
strong strong
spottedstrong expression: see section 05 moderate expression: see section 01 02 03 04 06 07 08 09
midgut
moderate moderate
spottedmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30668
Entity Detected:Gabbr1, gamma-aminobutyric acid (GABA) B receptor, 1 ( MGI:1860139)
Sequence:sense strand is shown

>T30668
GGGCCATGAACTCCTCCTCCTTTGAGGGTGTTTCTGGCCACGTGGTCTTTGATGCCAGCGGCTCCCGGAT
GGCATGGACGCTTATCGAGCAGCTACAGGGCGGCAGCTACAAGAAGATCGGCTACTACGACAGCACCAAG
GATGATCTTTCCTGGTCCAAAACAGACAAGTGGATCGGAGGGTCTCCCCCAGCCGACCAGACCTTGGTCA
TCAAGACATTCCGTTTCCTGTCACAGAAACTCTTTATCTCCGTCTCAGTTCTCTCCAGCCTGGGCATTGT
TCTTGCTGTTGTCTGTCTGTCCTTTAACATCTACAACTCCCACGTTCGTTATATCCAGAACTCCCAGCCC
AACCTGAACAATCTGACTGCTGTGGGCTGCTCACTGGCACTAGCTGCTGTCTTCCCCCTTGGGCTGGATG
GTTACCACATAGGGAGAAGCCAGTTCCCATTTGTCTGCCAGGCCCGCCTTTGGCTCTTGGGCTTAGGCTT
TAGTCTGGGCTATGGCTCTATGTTCACCAAGATCTGGTGGGTCCACACAGTCTTCACAAAGAAGGAGGAG
AAGAAGGAACGGAGGAAGACCCTAGAGCCTTGGAAACTGTACGCCACTGTAGGCCTGCTGGTGGGCATGG
ATATCCTGACTCTTGCCATCTGGCAGATTGTGGACCCCTTGCACCGAACCATTGAGACTTTTGCCAAGGA
GGAACCAAAGGAAGACATTGATGTCTCCATTCTGCCCCAGCTGGAGCACTGCAGCTCCAAGAAGATGAAT
ACATGGCTCGGCATTTTCTATGGTTACAAAGGGCTGCTGCTGCTGCTGGGAATCTTTCTTGCTTATGAGA
CCAAAAGTGTGTCCACTGAAAAGATCAATGACCACAGGGCCGTGGGCATGGCTATCTACAATGTTGCGGT
CCTGTGTCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6821442), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59670. Forward Primer - name:059670_F_IRAV117_c12_Gabbr1, sequence:GGGCCATGAACTCCTCCT; Reverse Primer - name:059670_R_SP6_IRAV117_c12_Gabbr1, sequence:TGAGACACAGGACCGCAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7734 same embryo
 EMAGE:7735 same embryo
 EMAGE:7732 same embryo
 EMAGE:7733 same embryo
 EurExpress:euxassay_009799 same experiment
 MGI:4824961 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS