EMAGE mouse gene expression spatial database

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EMAGE:7736

Wdr37 WD repeat domain 37 ( MGI:1920393)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007507. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007507_01	euxassay_007507_02	euxassay_007507_03	euxassay_007507_04

euxassay_007507_05	euxassay_007507_06	euxassay_007507_07	euxassay_007507_08	euxassay_007507_09

euxassay_007507_10	euxassay_007507_11	euxassay_007507_12	euxassay_007507_13	euxassay_007507_14

euxassay_007507_15	euxassay_007507_16	euxassay_007507_17	euxassay_007507_18	euxassay_007507_19

euxassay_007507_20	euxassay_007507_21	euxassay_007507_22	euxassay_007507_23	euxassay_007507_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7736_wholemount_strong.wlz

7736_wholemount_moderate.wlz

7736_wholemount_weak.wlz

7736_wholemount_possible.wlz

7736_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7736_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 07 08 09 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 18 20 21

trigeminal v nerve

moderate

regional

moderate expression: see section 09 10 17 18

spinal cord

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 weak expression: see section 17

cervical ganglion

moderate

regional

moderate expression: see section 09 18

thoracic ganglion

moderate

regional

moderate expression: see section 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 17 18 19

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9064
Entity Detected:	Wdr37, WD repeat domain 37 ( MGI:1920393)
Sequence:	sense strand is shown >T9064 AGCTTATTGCAGGAGTGACCAGGACACTACCTTCTAGAAGTAATGCCCACAGAAAGCGGAAGTTGTTCAA CTGCTCGCCAAGCAAAACAAAAGCGCAAGTCTCATAGCCTTTCTATACGAAGAACTAACAGCTCAGAACA AGAGAGGACTGGACTGCCAAGAGAAATGTTAGAAGGACAAGATTCTAAACTGCCGTCCTCAGTTCGCAGT ACACTGCTGGAATTGTTTGGCCAGATAGAAAGAGAATTTGAAAATCTTTACATCGAAAACTTAGAATTGC GCAGAGAAATTGATACTCTTAATGAACGTTTAGCTGGTGAAGGACAAGCGATCGATGGGGCAGAATTGAG TAAGGGCCAACTCAAAACAAAAGCCAGTCACAGTACCAGTCAGCTTTCTCAGAAGCTTAAGACCACCTAC AAGGCTTCCACCAGCAAGATTGTTTCCAGCTTTAAGATTACCACATCCAGGGCCATCTGTCAGCTTGTGA AGGAGTATATTGGCCACAGGGATGGCATCTGGGATGTCAGTGTTACAAGGACACAGCCTATTGTTCTTGG GACAGCATCTGCTGATCACACGGCTTTGTTGTGGAGCATAGAGACTGGCAAATGTCTTGTCAAGTACGCT GGCCATGTGGGCTCAGTAAATTCTATAAAATTCCATCCCTCAGAGCAGTTGGCTCTTACTGCTTCTGGAG ATCAGACTGCTCATATCTGGAGATACGTGGTACAGCTTCCAACACCTCAGCCTGTTGCCGACACTAGTCA ACAAATTTCTGGTGAAGATGAAATAGAATGCTCTGATAAAGATGAGCCTGACATTGATGGAGACGTGTCC AGTGACTGTCCTACTGTCCGAGTCCCACTGACATCTCTCAAAAGCCACCAGGGTGTGGTTATTGCTGCTG ACTGGCTGGTTGGTGGGAAACAAGTGGTGACAGCCTCATGGGACAGAACTGCCAACCTGTATGATGTGGA GACATCTGAACTCGTTCATTCCCTGACAGGGCATGACCAGGAGTTAACACATTGTTGCACTCACCCCACC CAGCGGCTCGTGGTGACCTCCTCCCGTGACACAACTTTCCGTCTCTGGGATTTCAGGGACCCTTCCATCC ACTCCGTGAATGTTTTCCAAGGACACACGGACACTGTGACCTCTGCTGTGTTCACTGTGGGAGACAACGT CGTTTCAGGCAGTGATGACCGCACAGTAAAAGTCTGGGATTTAAAAAATATGAGATCTCCAATTGCGACA ATTCGTACAGACTCTGCCATTAATAGGATCAATGTGTGTGTTGGCCAAAAAATCATAGCCCTCCCCCATG ACAACCGGCAAGTGCGATTGTTTGATATGTCGGGAGTGAGGCTAGCACGGCTTCCCCGGAGCAGCCGACA GGGTCACAGACGAATGGTGTGCTGCTCAGCGTGGAGTGAAGACCACCCCATATGCAATCTATTTACCTGT GGGTTCGATAGGCAAGCTATTGGGTGGAACATCAATATTCCTGCACTGTTACAAGAAAAATAAGTCACTG ATGTTCCTTAGCTCCAAGTTTGCCATGGACTTTTGATTCATGCAGGCTCTTCTGCATATTAATCAGCCAT TATAGTGAGAGTTTGACCCTGGGAAGGGTGCCTTGTATATGTTCTTTTCACATTGTGCCCAGCTTGCATG AAGTGTACAGAGAGATGTGTGATTCTGTTTTTTATTTTTGTCTTGTGTATAATGGCATCCTCTGGTCAGT AGAAGTGAAGCTCACTCCATGTAGCATACAAAATGCTTTGAGTTGTTGAAGCTGACAGATGAGCAGTAGG GCATTACATTACATTGTATGAATCAAATGTGAACTGTGTGTCTATTGTGAGGCATAAACAGTCTGTTACA TTGTCTGGAGTAACTAGACATAACTACCTTGTCCTGGGAAGACTGCAGAGCTCATTTGCATTGGCTAGCA CGAGGCAGGACTGACAGATTTCATTCTTACTTGGTTTATGTGAAGTTGTAGAACGTGCTGTTGTGTAATT TCATTTCGACTCTTGACTTCTGTTCCGGTCCTGTAAAAGAAACCTGGATTTGCAACGTTTTGTGCAGAAT TTCTTTTGAGAAGAAAATGTTTCTGCTATTTTGTAGAAAATGATTTCAATCTCTTGAGGTCTCATACAAG CAAATTTTAAAATACGATTATTCTAATCATCGATTTTAGACATTAAACAGAATTCAAAGCACTTTAATTT ATAGCTGTGGTTATAAACAGTTTTTAGAAGTTTCAAATGATTTATCCATTGAATGTGACTTGATCTTTAT ACAAAGGCCCTTCTACCTGAAGACAAAATATTATTTATTTTCTACATCTTTGGTTTTCATATTTCTAAAT GGGTTCACTTCCTTGGTGGTGTTTGGAGAGCCAAGAATGCAAATAAATGAGCACTACCTCAAAATAAATA TTGGGAAACCTTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6817089 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6817089 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7738 same embryo
	EMAGE:7737 same embryo
	EMAGE:7740 same embryo
	EMAGE:7739 same embryo
	EurExpress:euxassay_007507 same experiment
	MGI:4829198 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source