EMAGE mouse gene expression spatial database

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EMAGE:7738

Cartpt CART prepropeptide ( MGI:1351330)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007423. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007423_01	euxassay_007423_02	euxassay_007423_03	euxassay_007423_04

euxassay_007423_05	euxassay_007423_06	euxassay_007423_07	euxassay_007423_08	euxassay_007423_09

euxassay_007423_10	euxassay_007423_11	euxassay_007423_12	euxassay_007423_13	euxassay_007423_14

euxassay_007423_15	euxassay_007423_16	euxassay_007423_17	euxassay_007423_18	euxassay_007423_19

euxassay_007423_20	euxassay_007423_21	euxassay_007423_22	euxassay_007423_23	euxassay_007423_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7738_wholemount_strong.wlz

7738_wholemount_moderate.wlz

7738_wholemount_weak.wlz

7738_wholemount_possible.wlz

7738_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7738_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 06 07 19 20 21 22 23 24 weak expression: see section 08 09 10 11 15 16 18

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 08 11 12 14 moderate expression: see section 07 15 18

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 18

pons mantle layer

strong

regional

strong expression: see section 08 18

ventral grey horn

strong

regional

strong expression: see section 11 12 13 14 15 16 moderate expression: see section 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 11 16 17

cervical ganglion

strong

regional

strong expression: see section 08 09 17

thoracic ganglion

moderate

regional

moderate expression: see section 14 15

nasal cavity olfactory epithelium

strong

single cell

strong expression: see section 11 12 14 15 16

mandible

moderate

regional

moderate expression: see section 07 08 09 16 weak expression: see section 03 04 05 06 10 11 15 17 18 19 20 21 22

maxilla

moderate

regional

moderate expression: see section 09 weak expression: see section 10 17 18 19 20 21

left lung

moderate

spotted

moderate expression: see section 03 04 05 06 07 08 09 10 11 12

right lung

moderate

spotted

moderate expression: see section 14 15 16 18 19 20 21 22 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 22 23 24 weak expression: see section 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9068
Entity Detected:	Cartpt, CART prepropeptide ( MGI:1351330)
Sequence:	sense strand is shown >T9068 CAGAACCATGGAGAGCTCCCGCCTGCGGCTGCTACCCCTCCTGGGCGCCGCCCTGCTGCTACTGCTACCT TTGCTGGGTGCCCGTGCCCAGGAGGACGCCGAGCTGCAGCCCCGAGCCCTGGACATCTACTCTGCCGTGG ATGATGCGTCCCACGAGAAGGAGCTGCCAAGGCGGCAACTTCGGGCTCCCGGCGCTATGTTGCAGATCGA AGCGTTGCAAGAAGTCCTGAAGAAGCTCAAGAGTAAACGCATTCCGATCTACGAGAAGAAGTACGGCCAA GTCCCCATGTGTGACGCTGGAGAGCAGTGCGCAGTGAGGAAAGGGGCCAGGATCGGGAAGCTGTGTGACT GTCCCCGAGGAACTTCCTGCAATTCTTTCCTCTTGAAGTGCTTGTGAAGGGACGACAGCCGCCACCTTCG GTTCCCATATTCCCTCTTTCCCCCAAAGGAGCGCTCCATTATCCCTGGAGCCTGGCTTTAGCAACAATAA AGTTTGCGTTCCCCTCAGAGAGCGGATGGGCTCTTTCCCTGTTGCTTCAAAATAAAAGATTTGACATCAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6817832 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6817832 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7737 same embryo
	EMAGE:7740 same embryo
	EMAGE:7739 same embryo
	EMAGE:7736 same embryo
	EurExpress:euxassay_007423 same experiment
	MGI:4823638 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source