EMAGE mouse gene expression spatial database

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EMAGE:7745

Tdg thymine DNA glycosylase ( MGI:108247)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007506. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007506_01	euxassay_007506_02	euxassay_007506_03	euxassay_007506_04

euxassay_007506_05	euxassay_007506_06	euxassay_007506_07	euxassay_007506_08	euxassay_007506_09

euxassay_007506_10	euxassay_007506_11	euxassay_007506_12	euxassay_007506_13	euxassay_007506_14

euxassay_007506_15	euxassay_007506_16	euxassay_007506_17	euxassay_007506_18	euxassay_007506_19

euxassay_007506_20	euxassay_007506_21	euxassay_007506_22	euxassay_007506_23	euxassay_007506_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7745_wholemount_strong.wlz

7745_wholemount_moderate.wlz

7745_wholemount_weak.wlz

7745_wholemount_possible.wlz

7745_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7745_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9055
Entity Detected:	Tdg, thymine DNA glycosylase ( MGI:108247)
Sequence:	sense strand is shown >T9055 CGCCGCGGTGATGTAGCAGCGTGGGAGGGGCCGAGGCCAGCGCCGTCCATCCACCGGCCGCCCCGATCCT GTGGTGAGTCCGCGCGCTGAGCGGCAGAGCGGGCGCCCGTGGGCTCGGCAGGAGTGGCCGTACCCTCGGC GGCGCCGCCATGGACGCAGAGGCCGCGCGCAGCTATTCTCTGGAGCAAGTTCAAGCTTTGTATTCATTTC CATTTCAACAAATGATGGCAGAAGTTCCTAACATGGCAGTCACGACTGGACAGCAGGTGCCAGCAGTAGC TCCTAACATGGCAACCGTGACAGAACAGCAGGTGCCCGAAGACGCTCCTGTCCAGGAACCTGCACCAGAA GCTCCAAAGAGAAGGAAAAGGAAACCCAGAGCAGCAGAGCCCCAGGAACCAGTGGAGCCCAAAAAACCTG CTACGTCGAAGAAATCCGGCAAGTCTACAAAATCAAAGGAAAAGCAGGAGAAAATCACAGACGCGTTTAA AGTGAAAAGGAAAGTGGACCGCTTCAACGGCGTCTCTGAAGCTGAGCTTCTGACCAAGACTCTTCCTGAC ATTTTGACCTTCAATCTGGATATTGTGATCATTGGCATTAACCCGGGATTAATGGCTGCTTACAAAGGAC ATCACTACCCTGGGCCTGGAAATCACTTCTGGAAGTGTCTGTTCATGTCGGGGCTGAGTGAGGTGCAGCT GAATCACATGGATGACCACACCTTACCCGGCAAGTACGGCATCGGATTCACCAACATGGTGGAACGGACG ACGCCGGGCAGCAAGGATCTGTCTAGTAAAGAGTTCCGGGAAGGAGGGCGCATCCTGGTGCAGAAACTGC AGAAATATCAGCCACGAATAGCGGTGTTTAATGGAAAATGTATTTATGAAATTTTCAGTAAAGAAGTTTT TGGAGTAAAGGTTAAGAACTTGGAATTTGGGCTTCAACCCCACAAGATCCCAGACACAGAAACTCTGTGC TACGTCATGCCGTCGTCCAGCGCCAGATGTGCTCAGTTTCCCCGGGCCCAGGACAAAGTTCATTACTACA TTAAGCTGAAGGACTTGAGAGACCAACTGAAAGGCATTGAACGCAACGCGGACGTTCAGGAAGTGCAGTA TACATTTGACCTGCAGCTTGCGCAAGAGGACGCAAAGAAGATGGCTGTTAAGGAAGAAAAGTATGATCCA GGCTATGAGGCAGCTTACGGCGGTGCCTATGGGGAAAACCCATGTAATGGGGAACCTTGTGGCATTGCTT CAAATGGGCTAACAGCTCACAGTGCGGAGCCGAGAGGAGAAGCGGCCCCCAGCGATGTTCCGAATGGGCA GTGGATGGCACAGTCGTTTGCAGAGCAGATCCCTTCTTTTAATAATTGTGGGACCCGAGAGCAGGAAGAA GAGAGCCACGCTTAGGAAGGGTGCCTCTCAGCTCGGGCTCAGGGCTGCAGTTCTAATGCAGACCTCAGGA CGGAGAGCCTCAGTGTGCTAACTGAGGGGCTTTAGTGTTAATGGTCCTTTACGCTAGTGGTGTAATTATA TATGGAGCAGTTATATGGTAGAGTCAACTGTATGAATCTATGTAGTTTGGAAAGGAAAAATAGGGTGTTT TGTACTTGAGTTTTGTATTTTAACTACCCATCATTCCAGCTTTTTATATACTATATTTCATTTATGAAGA AACCGGTTTTCTCTGAGGAGTCACTTCAATCTGTAATTTTAAAAAAGCAACAGTCGGAATATTTATAGTT GATTCTTCACTGTGCATACCTATATATGCCCTTATCCCTTTAAATGCCTAAGTAGGGCATGCTACTAAGA ATCAAGCTGGTGAAAGGCAGATGTGCCTATCTCTGTTGGAAGCCGACTAACCCTCCGCCCTCCGTGGACT CGAAGCTGGAGGTGGACTCAGAACCCTCTGGGTTGCAGTCACACGCAGACCCCCTTACTCTCCCAGTAGT GTGTGTGGCAGTTGCTTGGTAGTAGAAACCTAGTGGGGCCTGAGCCCTGCCGTTGGCCTCTGCCTCTTGT GGTTCCTGAGAAGGCAGTAGAACCTGGACAGTTGCCACCCCTCTGTTTTACTGAATCCCCCGTCAGTGTT CATCACTGTTGTGATTTTATCCCGACATGGAAAGCGCTAAGAAATGAACTTTCCAGTTAGCCACGCGCTG CTGCAGGTGTTTGATAATCCTGTTTAAACTGGTGCTGTGTACATGGGATGTACTGATACAGAACTTCTCT TCTAGGGAAAGCTAATCATTTGTCAATGATTTAAAAATTATTGTCACTACTGTTGATTACTGTGGACTAA GTGAGCCTCGTGAAAGAGCTGATTGGAATTTGTACCTTTGTGGCTGCTCATGCATTTTCCATGGTGCTAC AGACAGCCTGCCCGGGCTGGTGGCTAGGAAACGTGCGTGTTCAGCGATGCCTAGCGCTCTCACTCCTGAG CACTCTGACTTGGTGCCCACCCCTGGCGCGTGCTGGTTTGGCAGTATGAAATGCCCACGAGGGTTTTGTA TCTCACAAAGGTTATGTAGCCTGTTCTGTGAACAATGAGGGAAGCAGTCTCTTGGTGGAAATTTTAACAT GCCTTTAGCTGTGGCAGTGATGGGCTTTCTTTTTCCTTCTGTCTGGGTCAGGTTGTGCACAAGTCATGTT ATTTGGAAGGTGCACTGCACTGCTGTTTACATGGGCATTTTATGCGGGTGCTTTGAAGTGCCTTGCATCA GGGATTAGGATCAATTGAATTCTTTTTTTCATGGGACTATGTAAAGCATGTAGTATTGCCTTGGTATGTA ACTATTGTAGTTTTACCATGGAGAAATACCTTTAAATTATAACTCTGGAGAGCAGCCATGAGGACCCTCT GTACTTCCTGGTGCTTTTCCTTAACATGGTGGGTGAGTAGCGAGTGCGGGGCACCCTGTACATGGCAGGG TGGGGCGTTCTCATTAAATAATACTGAGATTTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6816716 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6816716 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7744 same embryo
	EMAGE:7746 same embryo
	EMAGE:7742 same embryo
	EMAGE:7741 same embryo
	EMAGE:7743 same embryo
	EurExpress:euxassay_007506 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source