EMAGE mouse gene expression spatial database

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EMAGE:7749

Mbp myelin basic protein ( MGI:96925)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007522. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007522_01	euxassay_007522_02	euxassay_007522_03	euxassay_007522_04

euxassay_007522_05	euxassay_007522_06	euxassay_007522_07	euxassay_007522_08	euxassay_007522_09

euxassay_007522_10	euxassay_007522_11	euxassay_007522_12	euxassay_007522_13	euxassay_007522_14

euxassay_007522_15	euxassay_007522_16	euxassay_007522_17	euxassay_007522_18	euxassay_007522_19

euxassay_007522_20	euxassay_007522_21	euxassay_007522_22	euxassay_007522_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7749_wholemount_strong.wlz

7749_wholemount_moderate.wlz

7749_wholemount_weak.wlz

7749_wholemount_possible.wlz

7749_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7749_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

strong

spotted

strong expression: see section 12 13 14 15

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 19 weak expression: see section 07

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 08 09 18 19

trigeminal v nerve

moderate

regional

moderate expression: see section 08 18 19

basal columns

strong

spotted

strong expression: see section 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 17 18 19

brachial plexus

strong

regional

strong expression: see section 05 06 07 08 09 19 20 21 22 23 moderate expression: see section 03 04 10

esophagus

moderate

regional

moderate expression: see section 13 14 weak expression: see section 15

left lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14

right lung

moderate

regional

moderate expression: see section 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3335
Entity Detected:	Mbp, myelin basic protein ( MGI:96925)
Sequence:	sense strand is shown >T3335 TGGCCTCGAGCCAGATTCGGACGAGGATTCCCTGCCTCCTAGAAAGGACTCATTCTTAGCTTTAGGGGGG TTCCTGTCACTGAATCGAGTCGCTGCCCTGGATGCAGGGCTGGCCTGGGCGACGCTCCAGGGATGAGGAG CTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCCTCTAACGTACTATGTCTTTTGATTT AGCATGCCTTCTGTAGACCTTCCAAAGAGCCCCACACTGGCACCGTCACCCCTAGGAAGGCAGGTGATGG TTGATGTAGCCCAATACTGCATCTTGTTAATCTGTTCTAACTCTGAGTAGAGTGTGGGTTTAAGATAACA CCGATTAATGTATCGCCACAATAACGTGAGGGTAAGAGAAAAAGCAGGGAAGAAATTTCCAGAAAAAAAC CCTCCAGATTGTCCCACGGGAGTGTTTGCCCTCCAGTGTGACTGAACGACCTTGCCCATGGCTTCGTCCA GACAGCGCAGCTGCAGTATGGCTGGACAGAAGCACCTACTATTCTTGAATATTGAAATAAAA
Notes:	The probe template was PCR amplified from IMAGE:315274 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:315274 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7748 same embryo
	EMAGE:7750 same embryo
	EMAGE:7747 same embryo
	EurExpress:euxassay_007522 same experiment
	MGI:4826122 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source