EMAGE mouse gene expression spatial database

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EMAGE:7763

Ppp1cb protein phosphatase 1, catalytic subunit, beta isoform ( MGI:104871)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007501. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007501_01	euxassay_007501_02	euxassay_007501_03	euxassay_007501_04

euxassay_007501_05	euxassay_007501_06	euxassay_007501_07	euxassay_007501_08	euxassay_007501_09

euxassay_007501_10	euxassay_007501_11	euxassay_007501_12	euxassay_007501_13	euxassay_007501_14

euxassay_007501_15	euxassay_007501_16	euxassay_007501_17	euxassay_007501_18	euxassay_007501_19

euxassay_007501_20	euxassay_007501_21	euxassay_007501_22	euxassay_007501_23	euxassay_007501_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7763_wholemount_strong.wlz

7763_wholemount_moderate.wlz

7763_wholemount_weak.wlz

7763_wholemount_possible.wlz

7763_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7763_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9028
Entity Detected:	Ppp1cb, protein phosphatase 1, catalytic subunit, beta isoform ( MGI:104871)
Sequence:	sense strand is shown >T9028 CGTGACTCGTAGGTGAGAACGCCGAGCCGCCGCCGCCGCCGAGAAGCCCTGTTAACGCTTTAGGGAGGAG AGTCTGGTGCCGACAAGATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGCCTGCTGGAGGT ACGAGGATGTCGTCCAGGAAAGATTGTACAAATGACTGAAGCAGAAGTCCGAGGGTTGTGTATCAAGTCT CGTGAAATCTTTCTCAGCCAGCCTATTCTTTTGGAATTGGAAGCACCACTGAAGATTTGTGGAGACATTC ATGGACAATATACAGACTTACTAAGATTATTTGAATATGGAGGTTTTCCACCAGAAGCCAACTATCTTTT CTTAGGAGATTATGTGGACAGAGGAAAGCAGTCTTTGGAAACCATCTGTTTGCTATTGGCTTACAAAATC AAATACCCGGAGAACTTCTTTCTTCTAAGAGGAAACCATGAGTGTGCTAGCATCAATCGCATTTATGGAT TCTACGATGAGTGTAAACGAAGATTTAATATTAAATTGTGGAAGACATTCACTGATTGTTTTAACTGTCT GCCTATAGCTGCTATTGTTGATGAGAAAATCTTCTGTTGTCATGGAGGACTGTCACCAGACCTACAATCT ATGGAACAGATTCGGAGAATTATGAGACCCACTGACGTACCTGATACAGGTTTGCTTTGTGACTTACTGT GGTCCGACCCAGATAAGGATGTGCAAGGCTGGGGAGAAAATGACCGTGGTGTTTCTTTTACTTTTGGAGC TGATGTAGTCAGTAAATTTCTGAATCGTCATGATTTAGACTTGATTTGTCGAGCTCATCAGGTGGTGGAA GACGGATATGAATTTTTTGCTAAACGACAGTTGGTAACCTTATTTTCTGCCCCAAATTACTGTGGCGAGT TTGACAATGCTGGTGGTATGATGAGTGTGGATGAGACTTTGATGTGTTCATTCCAGATATTGAAACCATC TGAAAAGAAAGCTAAGTACCAGTATGGTGGGCTGAATTCTGGACGTCCTGTCACTCCGCCTCGAACAGCT AATCCACCGAAGAAAAGGTGAAGACAGGAATTCTAGAAAGAGAAACCATCAGATTTGTTAAGGACATACT TCATAATATATAAGTGTGCACTGTAAAACCATCCAGCCATTCGACACCCTTTATGATGTCACACCTTTAA CTTAAGGAGACGGTAAAGGATCTTAAATTTTTTTCTAATAGAAAGATGTGCTACACTGTATTGTAATAAG TATACTCTGTTATAATATTCAACAAAATTAAATCCAAATTCAAAAGTATCCATTAAAGTTCTATCTTCTC ATATCACAGTTTTTAAAGTTGAAAAGCATCCCAGTTAAACTAGCCCTGTTAGTGACCCAGATGAAAGCAT GAAGATCCATCTGTGTAATGTGGTTTTAGTGGTGCTTGGTTGTTTCATTATTTTGAGCTTGTTTTGTTTT GTTTGTTTTTGCTAGAATAATGGCATCTACTTTTCCTATTTTTCCCTAAACATTTTTAAAAGTGAAAATG GGAAGAGCTTTAAAGACATTCACCAACTATTCTTTTCCTTTACTTATCTACTTAAGTAACTGTTGGATCT TACTAAGAAAACTTACCCCTCATTACAGTAAAAAGGAACTTTAGAGGTCGATAGGTTTTAAAAATATACA AACTATCTGATCCATTGATTTTAATCAAACAGTTTGACTGGGCAAACTTTGCAGCTGATAATGAGTATTT CCTTTTTACAAAATTGCCACTGATTTGGATTTGTGCACTCTAATCTTTAATTTATTGATGCTCTATTGTG CAGTAGCATTTCATTTAAGATAAGGCTCATATAGTAATATCCAAAACTAGTTGGTAATGTGATTATGTGG TACTTTGGCTTTGGGTTCTAATTCGCACGAAACACCTTTTGGCATGCTTAACTTTCTGGTATTACCCTCA CCTGCATTGGTTTTGTTTTTTGGGGTTTTTGTTGTTTGTTTGTTTTTAGATTCACAGAACATGAGAATCC TTTTTGACAAGCCTTGGATAGCTGGCTCTCTTCTTTCCCTCTCTCTATGTGAAGGATGTATTTAAATGAA CGCTGGTCAGTGGGACATTTGTCAGCTCTGAATATTGGGTGCTTCACTAATAATTGCCATGTGAATGTTG TTTTGACTGTAAGGCTATGTCACTAAAGATTTTTACTCTGCGTTTTCATAATCAAAGGTCATGATGTGTA TAGACATGCTTTGTAGTGAAGTATAGTAGCAATAATTTCTGTATGTGATCAAGAGTTTATTGCATTATTT CTTTCCCTGTTCTCTTTTTTTTTTTTTAAGGGTTAGCATTAACAAATGTCAAGGAGTAGCAAAGTCAACA AAGATTTTAGAAGGAGGAGGAACTAAGAGCATACACAGACTTATGATTCTTTGGATGTGACACTTATTGG ATGTGATTCTAAAGTCTTTTATTGAATATTGTCAAATTCGTACGCTTCATAGGATGGACATAATGTTTAT ATAATGCCCTTCTTATGTGTTACCATAGATGTGTGAAACCTTATAGCGTCCTTGAAAGTGTTAAATTGAG AACTCTGTTAACATTTTATGGATTGACACATTATATTACTGCAAGTAACATTTGATTTTCAGCACAGTGC AAAAGTTCTTTAAAATGCATATGTCTTTTTTTTTCTAATTCCATTTTGTTTAAAGCACATTTTAAATGTA GTTTTCTCATTTAGTAAAAGTTGTCTAATTGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6812937 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6812937 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7765 same embryo
	EMAGE:7764 same embryo
	EMAGE:7766 same embryo
	EMAGE:7767 same embryo
	EMAGE:7768 same embryo
	EurExpress:euxassay_007501 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source