EMAGE mouse gene expression spatial database

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EMAGE:7764

Med16 mediator complex subunit 16 ( MGI:2158394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007478. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007478_01	euxassay_007478_02	euxassay_007478_03	euxassay_007478_04

euxassay_007478_05	euxassay_007478_06	euxassay_007478_07	euxassay_007478_08	euxassay_007478_09

euxassay_007478_10	euxassay_007478_11	euxassay_007478_12	euxassay_007478_13	euxassay_007478_14

euxassay_007478_15	euxassay_007478_16	euxassay_007478_17	euxassay_007478_18	euxassay_007478_19

euxassay_007478_20	euxassay_007478_21	euxassay_007478_22	euxassay_007478_23	euxassay_007478_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7764_wholemount_strong.wlz

7764_wholemount_moderate.wlz

7764_wholemount_weak.wlz

7764_wholemount_possible.wlz

7764_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7764_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9025
Entity Detected:	Med16, mediator complex subunit 16 ( MGI:2158394)
Sequence:	sense strand is shown >T9025 GCGGCGTCGGATCCCGGGCGTTGGGGCGAGTGTGGACCGCGGCCGCCGGGCCGACTGGGCGCCCGGGGCG GACAGGAGCCAGGGACCGTGCTGGGTGCGGTGGGTATGATGGACCTGGCCTATGTGTGCGAATGGGAGAA GTGGGCCAAGAGCACGTACTGCCCGTCGCTGCCCCTGGCTTGTGCCTGGTCCTGCAGGAACCTCACTGCC TTCACCACAGACCTACGCAATGATGACCAGGATCTGACACACATGATCCATATCCTAGACACGGAACACC CCTGGGAAGTGCACTCTGTCAGCTCTGGCCACAGCGAGGCCATCACCTGCCTGGAGTGGGACCAGTCAGG GTCCCGGCTACTGTCTGCTGATGCTGATGGACAGATCAAATGCTGGAGCATGGCTGACCACCTGGCCAAC AGCTGGGAGAGCTCGGTGGGCAGCCAGGTGGAGGGGGACCCCATCGTGGCCCTGTCCTGGCTGCACAACG GTGTAAAGCTGGCCCTGCATGTGGAGAAGTCTGGTGCCTCTAGCTTCGGAGAGAAGTTCTCACGTGTGAA GTTCTCCCCGTCCCTCACGCTGTTCGGTGGCAAGCCAATGGAGGGTTGGATTGCAGTGACAGTCAGTGGC CTGGTCACTGTGTCCCTTCTGAAGCCCAGCGGGCAGGTGCTGACGTCCACGGAGAGCCTGTGCCGGCTGC GTGGTAGAGTGGCGCTGGCTGACATCGCCTTCACGGGCGGTGGGAACATTGTGGTGGCTGCTGCAGATGG CAGCAGCGCGTCACCCGTGAAGTTCTATAAGGTGTGCGTCAGCGTGGTCAGCGAGAAGTGCCGCATCGAC ACGGAGATCCTGCCCTCACTGTTTATGCGCTGCACCACCGACCCCAACCGCAAAGACAGGTTCCCCGCCA TCACACACCTCAAGTTCCTGGCGCGAGACATGTCTGAGCAGGTGCTCCTGTGTGCGTCCAGCCAGACCAG CAGCCTGGTGGAATGCTGGTCCCTTCGGAAAGAGGGCCTTCCTGTGAACAATATCTTCCAGCAGATCTCG CCTGTGGTTGGTGACAAACAGCCCATGATCCTCAAGTGGCGAATCCTGTCGGCCACCAATGACCTGGACC GTGTATCCGCGGTGGCACTGCCGAAACTGCCCATCTCACTCACCAACACTGACCTCAAGGTGGCCAGTGA CACCCAGTTCTATCCAGGCCTTGGCTTAGCGCTGGCCTTCCAGGATGGCAGCGTGCACATGGTGCACCGA CTGTCCCTGCAGACCTTGGCGGTGTTCTACAGCTCAGCCCCGCGCTCGCTGGATGAGCCGGCCCTAAAGC GCCTACGCACCACATGCCCTGCCGTACACTTTAAAGCCATGCAGCTGTCCTGGACTTCGCTGGCCCTCGT GGGCATCGATAACCATGGGAAGCTCAGCATGCTGCGAATCTCTCCATCCCTGGGCCACCCACTGGAGCCC AAGCTGGCCCTGCAGCACCTGCTTTTCCTGCTGGAATACTGCATGGTGACCGGCTATGACTGGTGGGACA TCCTGCTACACGTGCAGCCCGGCATGGTGCAGAGCCTGGTGGAGCGGCTGCATGAGGAGTACACTCGCCA GAAGCCCGCCCTGCAACAGGTCCTCTCCACTCGGATCCTGGCCATGAAGGCTTCACTGTGCAAGCTGTCA CCCTGCACAGTGGCTCGTGTGTGTGACTACCACACCAAGCTGTTCCTCATGGCCATCACGTCCACCCTAA AGTCGCTGCTGCGCCCACACTTCCTCAACACCCCTGACAAGAGCCCTGGGGACCGCCTGGCCGAGATCTG CGCCAAGATCACCGATGTTGACATCGACAAAGTCATGATCAACCTGAAGACTGAAGAGTTCGTCCTTGAC ATGAACACTTTGCAGGCGCTGCAGCAGTTGCTACAGTGGGTGGGAGACTTCGTGCTCTACCTCCTGGTCA GCCTGCCCAACCAGGGCTCCCCGCTAAGGCCGGGCCACAGCTTCCTCCGAGATGGTACCTCCCTGGGCAT GCTGCGAGAGTTGATGGTTGTCATCCGAATCTGGGGCCTGCTGAAGCCCAGCTGTCTGCCCGTCTACACA GCCACCTCGGACACCCAGGACAGCATGTCCCTGCTCTTCCGACTGCTCACCAAACTGTGGATCTGCTGCC GTGATGAGGGCGCAGCCAGTGAACCAGATGAGGGTTTGGTGGATGAATGCTGCCTGCTGCCTAGCCAGCT GCTGGTCCCCAACTTGGACTGGCTTCCCGCCAGTGATGGCCTGGTCAGTCGCCTGCAGCCCAAACAGCCC CTGCGCCTGCGCTTCGGCAGAGCGCCCACTCTGCCCAGCAGCACCTCCACCCTGCAGCTGGACGGCCTCA CCAGGGCCCCTGGCCAGCCCAAGATCGACCACCTCCGGAGGCTACACCTGGGCGCCTACCCCACCGAGGA GTGCAAAGCCTGCACCAGGTGTGGCTGTGTCACCATGCTGAAGTCTCCCAACAAGACAACCGCTGTGACA CAGTGGGAACAGCGGTGGATCAAGAATTGCCTGTGTGGTGGGCTGTGGCGGCGAGTGCCTCTCAGCTGTT CCTGAGCCACAGCCCACCAGCCTGCGTATGTACAACCAGTCTCTGAACATTGACATTGCTGTGCTCACTG AGGCACAAGTACCCACCAATGGCCCCTGGGTCTGCCCCTTCTAGGGACATGCGGCATCTCTAGGGCCTGA AGTCCCATGGTCATATCCAAGCCAGTGTGAAGGAGCTGGGCTGTGTATTCATCCTTTAAAAGATCCACAG TGGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGTGGATTTCTGAGTT CGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAA AAACAAAACAAAAAAAAAAAAAGATTCACAGTACATAAAGCCCCTTGTCCTGCTTGGGGTCGTCTGCCTC AGTTTTCTTGTCCTGCTTGGGGTCGTCTGCCTCAGTTTTCTTGTCCTGCTTGGGGTCGTCTGCCTCAGTT TTCTTGTCCTGCTTGGGGTCGTCTGCCTCAGTTTTCTTTGGGTGCCGGTGGGAGGGGAAGACTGGCCCAA TAAAGTTCTCTGATGCACTAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6813010 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6813010 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7765 same embryo
	EMAGE:7766 same embryo
	EMAGE:7767 same embryo
	EMAGE:7763 same embryo
	EMAGE:7768 same embryo
	EurExpress:euxassay_007478 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source