EMAGE mouse gene expression spatial database

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EMAGE:7767

Arsg arylsulfatase G ( MGI:1921258)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007487. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007487_01	euxassay_007487_02	euxassay_007487_03	euxassay_007487_04

euxassay_007487_05	euxassay_007487_06	euxassay_007487_07	euxassay_007487_08	euxassay_007487_09

euxassay_007487_10	euxassay_007487_11	euxassay_007487_12	euxassay_007487_13	euxassay_007487_14

euxassay_007487_15	euxassay_007487_16	euxassay_007487_17	euxassay_007487_18	euxassay_007487_19

euxassay_007487_20	euxassay_007487_21	euxassay_007487_22	euxassay_007487_23	euxassay_007487_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7767_wholemount_strong.wlz

7767_wholemount_moderate.wlz

7767_wholemount_weak.wlz

7767_wholemount_possible.wlz

7767_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7767_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14

choroid fissure

strong

regional

strong expression: see section 14

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 15 16 17 18 19 20

4th ventricle

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 13

pons ventricular layer

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 15 16

midbrain ventricular layer

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9027
Entity Detected:	Arsg, arylsulfatase G ( MGI:1921258)
Sequence:	sense strand is shown >T9027 CCAACTCTGGTTTCTTGTCAGATTTTGTTCCTCCAGGAAACCTTACAGAAACCTCTACCATCTGGAACTC AGAAACTCATCTCAGGGCTGACTGTGGCTTCTAGAACATGCAGGTGTTCTGCAGATGTGAGACTGTATCC CGCAGTCACCAGATGAAATCCTAAGCAGATGCAAGGCTTGCTTTGTGGCCTTAGCTCCCCTGAGTGCAAA GGGCCACTTCTCTGCTAAGTGGCCAAGAGTGGATATTGACATTGATGTCTGAATGCTGCACCCAGCCAGT CATCTTATTAAGCTGCGATGAGAAGACATCTTGAGGGGACCAACCCAACTTAGGGGTGACACATTCCTGA CTTTCTTGGATTAGGATTCCACATGGGGACCTTGTGTCTGTAGCCCTGTAGCCATTACTAACTCCAGTGT ATTCTTGACAACTGGACTCCTTGGAGACTTCCTGCACTGAAAGTGAACAAAGAGAAAACTCTAAAATTCC AGAGATTCACAGTGGTGCTCGGTTATCATTTATCCAAGACAACGGGAGTCTTCGTGTCTTACAATGGGCT GGCTCTTTCTAAAGGTTTTGTTGGTTGGCATGGCTTTTTCTGGATTTTTCTACCCTCTCGTGGATTTCTC TATCAGTGGGAAAACAAGAGCCCCCCAACCAAACATCGTGATCATTTTGGCTGATGACATGGGATGGGGT GATCTGGGAGCAAACTGGGCAGAAACAAAGGACACCACCAATCTTGACAAGATGGCTTCCGAAGGAATGA GGTTTGTAGACTTCCACGCAGCTGCTTCTACCTGCTCCCCATCCCGAGCCTCCCTGCTCACTGGCCGGCT GGGTCTACGGAACGGAGTCACGCACAACTTCGCAGTCACCTCTGTAGGGGGGCTTCCAGTCAACGAGACC ACCTTGGTAGAGGTGCTGCGACAGGAGGGTTACGTCACTGCCATGATAGGCAAGTGGCATCTTGGGCATC ATGGCTCTTATCATCCCAATTTCCGTGGCTTCGATTACTACTTTGGAATCCCATATAGCAATGACATGGG TTGCACTGATGCTCCAGGCTACAACTACCCTCCTTGTCCAGCGTGTCCACAGAGAGATGGCCTATGGAGG AACCCTGGGAGGGACTGTTACACAGATGTTGCCCTTCCTCTCTATGAAAACCTGAACATCGTGGAGCAGC CTGTGAACCTGAGCGGCCTTGCACAGAAGTACGCAGAAAGGGCAGTGGAGTTCATTGAGCAGGCAAGCAC CAGTGGAAGACCCTTCCTGCTCTACGTGGGCCTGGCTCACATGCATGTGCCTTTGTCTGTAACTCCACCA TTGGCACACCCACAGCGCCAAAGCCTGTACCGTGCAAGTCTCCGGGAGATGGACAGTCTGGTAGGGCAGA TCAAGGACAAAGTTGACCATGTGGCAAGAGAAAACACACTCCTCTGGTTTACAGGAGACAATGGCCCATG GGCTCAGAAGTGTGAGCTGGCAGGCAGCGTGGGTCCCTTCTTCGGGTTGTGGCAAACCCATCAAGGGGGA AGTCCAACCAAGCAGACCACCTGGGAAGGTGGGCATCGGGTTCCAGCACTGGCTTACTGGCCTGGCAGAG TTCCAGCCAACGTCACTAGCACCGCCTTGTTAAGCTTGCTAGACATCTTCCCCACCGTGATAGCTCTGGC AGGAGCCAGCTTGCCCCCAAACCGGAAATTTGATGGACGGGATGTCTCTGAGGTGCTCTTTGGCAAGTCA CAGATGGGGCACAGGGTGCTATTCCACCCCAACAGTGGGGCTGCTGGAGAGTATGGCGCCCTGCAGACTG TCCGCCTAAACCATTACAAAGCTTTCTACATCACTGGTGGAGCCAAAGCTTGTGATGGGAGCGTGGGTCC TGAGCAGCACCACGTGGCCCCTCTCATTTTCAACCTAGAAGATGCTGCTGATGAAGGGATGCCTCTACAG AAGGGGAGCCCCGAGTACCAGGAGGTGCTGCAGCAGGTCACCAGGGCTCTCGCAGATGTCCTCCAAGACA TCGCTGATGACAACAGCTCCCGAGCAGACTACACTCAGGATCCTTCAGTGATTCCTTGCTGTAACCCCTA TCAAACTACCTGCCGTTGCCAACCGGTCTGAGGGCTCATTGTTCCCCCCATGAGAAACACTACCTGAAAA CTGTAGACAGGTGGGTTTACTTCCAAACTTGGGTGTGGTCATGGATGTCTGCTTTAGAAAGGAAGCTGCA GCATTTGGTTAGAAAGCAACGGCGTTACCTCTGTGTGTGTGGACTGTCTCCTCTGCATGCAGACTTGAGA AGCCCTGAGTGCTGGTACAGTGGACACGGCATGGTTAAGCACTAGCGAGTAATGGGGTCAGGTCTGAGCC CTGGGTTTAAAGCTCAGGCAATGCCTTAAACCTCAATTGCAAGTTGAAGGTTAAAGTAACCCACGAAACT GTGTGTAACCCACGAACTGACACCAGGCAGATTCTCGATCGGTATTTTAGTTTCTATCTCGTGTCCCCAG TTTCAGGGGCCATTTTGGCACAGACTCGTCTGGTTTGCTGGTGGGTAGGGGTCCGGGAGCCTACACTGTT GCCTTTGAGATGACTTGAGCAAGCTAAACCAAAGTGAGGTTTTCCCAGGCCTTGGACCCTTCTCTCCTGT AAATGTCAAGTGGATGTATTATAAAGATGTAAGCATTCAGGGCTGAAGTGGTGGCCCGTTGGTTAAGAGC TTGGCCCACTTGCTCAGTTCCCAGCACCTACATGGTAGCATAAACTGTCTGCAACTCTAGCTCTAGGGGA ACCTTGTCCTCTTCTCTGGCCTCTGAGAATGCCAGACACACACGTGGTGTATATACATGTAGGTAAAACA CTCATATATTAAAACATACTCTTTTCAAAAAAATTGGGATTTCTTCCCAGCCCCTAAAACTTCTAAGAAA CAAAATAAAAATGTAAACAACTCACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6813261 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6813261 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7765 same embryo
	EMAGE:7764 same embryo
	EMAGE:7766 same embryo
	EMAGE:7763 same embryo
	EMAGE:7768 same embryo
	EurExpress:euxassay_007487 same experiment
	MGI:4823261 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source