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EMAGE:7782

Prdx1 peroxiredoxin 1 ( MGI:99523)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7782 EMAGE:7782 EMAGE:7782 EMAGE:7782 EMAGE:7782
"Pseudo-wholemount" of euxassay_007427. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007427_01 euxassay_007427_02 euxassay_007427_03 euxassay_007427_04
EMAGE:7782 EMAGE:7782 EMAGE:7782 EMAGE:7782 EMAGE:7782
euxassay_007427_05 euxassay_007427_06 euxassay_007427_07 euxassay_007427_08 euxassay_007427_09
EMAGE:7782 EMAGE:7782 EMAGE:7782 EMAGE:7782 EMAGE:7782
euxassay_007427_10 euxassay_007427_11 euxassay_007427_12 euxassay_007427_13 euxassay_007427_14
EMAGE:7782 EMAGE:7782 EMAGE:7782 EMAGE:7782 EMAGE:7782
euxassay_007427_15 euxassay_007427_16 euxassay_007427_17 euxassay_007427_18 euxassay_007427_19
EMAGE:7782 EMAGE:7782 EMAGE:7782 EMAGE:7782 EMAGE:7782
euxassay_007427_20 euxassay_007427_21 euxassay_007427_22 euxassay_007427_23 euxassay_007427_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7782Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7782_wholemount_strong.wlz
7782_wholemount_moderate.wlz
7782_wholemount_weak.wlz
7782_wholemount_possible.wlz
7782_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7782_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9074
Entity Detected:Prdx1, peroxiredoxin 1 ( MGI:99523)
Sequence:sense strand is shown

>T9074
TGTTTGTGAGACCTGTGGCTCGACCCTGCTGATAGCAAGATGTCTTCAGGAAATGCAAAAATTGGGTATC
CTGCTCCCAACTTCAAAGCTACAGCTGTTATGCCAGATGGACAATTCAAAGATATCAGCCTAAGTGAATA
CAAAGGAAAATATGTTGTGTTCTTCTTTTACCCTCTTGACTTTACTTTTGTGTGTCCCACGGAGATCATT
GCTTTCAGTGATAGAGCCGATGAATTTAAGAAACTCAACTGCCAAGTGATTGGCGCTTCTGTGGATTCTC
ACTTCTGTCATCTGGCATGGATTAACACACCCAAGAAACAAGGAGGATTGGGACCCATGAACATTCCCTT
AATATCAGATCCCAAGCGCACCATTGCTCAGGATTATGGAGTCTTAAAGGCTGATGAAGGTATCTCTTTC
AGGGGCCTTTTTATTATTGATGATAAAGGTATCCTTCGACAGATAACAATAAACGATCTTCCCGTTGGCC
GCTCTGTGGATGAGATTATACGACTAGTCCAGGCCTTCCAGTTCACTGACAAACATGGTGAAGTGTGTCC
AGCTGGCTGGAAACCTGGCAGTGATACCATCAAGCCTGATGTCAATAAGAGCAAAGAGTATTTCTCTAAG
CAGAAGTGAGCACTGGACCATTTTTCTGCCAGGCAGCATTGAGCAGCCAGAAGAAACTCTTGTACTCTAC
TCGTGCTTAAACACATGATGTGGTGTGATTCCAGATAAGCCTTTCCTACAGGGCTGGGGATGGATAGCCT
TTCTTCCACTATTGGTAATGGTCTGAGCTGTGTTTTGGGCAGACCAATCTTCTATCAGTCACAGAAAACA
ACCTGTTAATTCTTTTTTCTTCTTTTTTTAAGTATCTATTAAACGTGAATTCTGAGAAAAAAAAAAAAAA
AAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6819164 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6819164 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7785 same embryo
 EMAGE:7784 same embryo
 EMAGE:7783 same embryo
 EMAGE:7781 same embryo
 EurExpress:euxassay_007427 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS