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EMAGE:7851

AI848100 expressed sequence AI848100 ( MGI:2138346)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7851 EMAGE:7851 EMAGE:7851 EMAGE:7851 EMAGE:7851
"Pseudo-wholemount" of euxassay_007710. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007710_01 euxassay_007710_02 euxassay_007710_03 euxassay_007710_04
EMAGE:7851 EMAGE:7851 EMAGE:7851 EMAGE:7851 EMAGE:7851
euxassay_007710_05 euxassay_007710_06 euxassay_007710_07 euxassay_007710_08 euxassay_007710_09
EMAGE:7851 EMAGE:7851 EMAGE:7851 EMAGE:7851 EMAGE:7851
euxassay_007710_10 euxassay_007710_11 euxassay_007710_12 euxassay_007710_13 euxassay_007710_14
EMAGE:7851 EMAGE:7851 EMAGE:7851 EMAGE:7851 EMAGE:7851
euxassay_007710_15 euxassay_007710_16 euxassay_007710_17 euxassay_007710_18 euxassay_007710_19
EMAGE:7851 EMAGE:7851 EMAGE:7851 EMAGE:7851 EMAGE:7851
euxassay_007710_20 euxassay_007710_21 euxassay_007710_22 euxassay_007710_23 euxassay_007710_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7851Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7851_wholemount_strong.wlz
7851_wholemount_moderate.wlz
7851_wholemount_weak.wlz
7851_wholemount_possible.wlz
7851_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7851_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35909
Entity Detected:AI848100, expressed sequence AI848100 ( MGI:2138346)
Sequence:sense strand is shown

>T35909
TATGCAACGCTGTCGAACTACTTCTCAATTTGATGGAGATTACATTTCAAAACTTCCCAAAAGTAATCAG
TATCCAAGCCCTAAAAGGTGTTTCTCTTCCTATGATGACATGAATTTGAAAAGAAGAACATCATTTCCCC
TCATCAGATCCAAATCTCTGCAGTTTACTGGCAAAGAAGTAGATCCGAATGATTTGTACATCGTAGAACC
CCTCAAGTTCTCTCCAGAGAAAAAGAAGAAACGTTGCAAGTACAAAACTGAAAAAATTGAGACAATAAAG
CCTGCAGATCCATTGCACCCTATAGCAAATGGAGATATAAAAGGAAGAAAGCCCTTTACGAACCAGAGAG
ATTTTTCTAACATGGGAGAAGTTTATCACTCTTCTTACAAGGGCCCTCCGTCTGAGGGCAGCTCGGAAAC
TTCATCACAGTCAGAAGAGTCCTATTTTTGTGGCATTTCAGCTTGTACAAGTCTGTGCAATGGACAGACC
CAAAAGACAAAAACTGAGAAGAGGGCTTTAAAACGAAGGCGATCTAAAGTCCAAGACCAAGGAAAGTTAA
TAAAAGCTCTTATACAGACTAAGTCGGGGTCATTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75982. Forward Primer - name:075982_F_cDNA_AI848100, sequence:TATGCAACGCTGTCGAACTACT; Reverse Primer - name:075982_N_SP6_cDNA_AI848100, sequence:CAATGACCCCGACTTAGTCTGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7849 same embryo
 EMAGE:7848 same embryo
 EMAGE:6094 same embryo
 EMAGE:7850 same embryo
 EMAGE:6095 same embryo
 EurExpress:euxassay_007710 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS