EMAGE mouse gene expression spatial database

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EMAGE:7879

C130021I20Rik Riken cDNA C130021I20 gene ( MGI:3639863)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012810. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012810_01	euxassay_012810_02	euxassay_012810_03	euxassay_012810_04

euxassay_012810_05	euxassay_012810_06	euxassay_012810_07	euxassay_012810_08	euxassay_012810_09

euxassay_012810_10	euxassay_012810_11	euxassay_012810_12	euxassay_012810_13	euxassay_012810_14

euxassay_012810_15	euxassay_012810_16	euxassay_012810_17	euxassay_012810_18	euxassay_012810_19

euxassay_012810_20	euxassay_012810_21	euxassay_012810_22	euxassay_012810_23	euxassay_012810_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7879_wholemount_strong.wlz

7879_wholemount_moderate.wlz

7879_wholemount_weak.wlz

7879_wholemount_possible.wlz

7879_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7879_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand skin

strong

regional

strong expression: see section 22 23 24 moderate expression: see section 02 03 04

foot skin

strong

regional

strong expression: see section 05 06 22 23 24 moderate expression: see section 02 03 04 20 21

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

diencephalon roof plate

strong

regional

strong expression: see section 14

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 17 moderate expression: see section 08 09 10 11 13 14 15 16

pons mantle layer

strong

regional

strong expression: see section 06 07 12 17 18 19 moderate expression: see section 08 09 10 11 15 16

midbrain mantle layer

strong

regional

strong expression: see section 09 11 12 13 14 15

dorsal grey horn

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38182
Entity Detected:	C130021I20Rik, Riken cDNA C130021I20 gene ( MGI:3639863)
Sequence:	sense strand is shown >T38182 GCATCTCAAGATCTACGAGGCTCTGCCGCGCAGAATCAAAGCTGTCCCAAAACTGTGGGCCAAGTCGGGA GCTGCTCCTCGGGGAAACTCAGTCTATCCCAGACCCTCAGTGATGAAGAACCCGAAGACCACGGAAATGG AGCAAGGGCGCGCCCAAGACTCAGCAGAGGTCGATCACTAGCAATCCAACTCCCGAGCATCCAGGTACCC CTCTGAGCATTCTAGCTGGTCTTCACCCCACACACGAGTCAAAGCATTGGACGACCACTTTCTACTACCT AAGGAAGGGCGTCTGAAGTCTCCCTGTGCTGGTGGAAGTTCTCCAGAGAGGCGAGACTAGAGAGGGGTTT CTGGGGTACTTATTCTCCACTGGGGGCTTTTGAGGCTGATGAGAGAGGGGTGTCAGGGTTTGCTGGTGGC TGGTGAGTGTTAGGATGCCTAGAGCCAGAAGATGAACATCCGCAGCCAGATCCCTCAGCCCTCCAACAAC CCCCACTCTGGCCATTACTCTGGACCATCAGGCATGGACTTGCTCCACTTGTGGGGAAATCAGGCTCTCT CAGATCCAAAGGCTGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105507. Forward Primer - name:105507_F_cDNA_C130021I20, sequence:GCATCTCAAGATCTACGAGGCT; Reverse Primer - name:105507_N_SP6_cDNA_C130021I20, sequence:GCAGCCTTTGGATCTGAGAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7881 same embryo
	EMAGE:7877 same embryo
	EMAGE:7880 same embryo
	EMAGE:7876 same embryo
	EMAGE:7878 same embryo
	EurExpress:euxassay_012810 same experiment
	MGI:4823528 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source