EMAGE mouse gene expression spatial database

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EMAGE:7889

Shisa7 shisa homolog 7 (Xenopus laevis) ( MGI:3605641)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012823. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012823_01	euxassay_012823_02	euxassay_012823_03	euxassay_012823_04

euxassay_012823_05	euxassay_012823_06	euxassay_012823_07	euxassay_012823_08	euxassay_012823_09

euxassay_012823_10	euxassay_012823_11	euxassay_012823_12	euxassay_012823_13	euxassay_012823_14

euxassay_012823_15	euxassay_012823_16	euxassay_012823_17	euxassay_012823_18	euxassay_012823_19

euxassay_012823_20	euxassay_012823_21	euxassay_012823_22	euxassay_012823_23	euxassay_012823_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7889_wholemount_strong.wlz

7889_wholemount_moderate.wlz

7889_wholemount_weak.wlz

7889_wholemount_possible.wlz

7889_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7889_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 04 16 17 18 19 20 21 22 23 24

diencephalon lateral wall mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 weak expression: see section 07 08 16 18

cerebral cortex mantle layer

weak

regional

weak expression: see section 04 05 06 19 20 22 23 24

telencephalon mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 weak expression: see section 01 02 03 04 05 06 07 08 18 19 20 21 22 23 24

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 10 11 12 15 16 17 weak expression: see section 18 19

medulla oblongata alar plate mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 weak expression: see section 07 08

medulla oblongata basal plate mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 weak expression: see section 06 07 08

rest of cerebellum mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 weak expression: see section 04 05 06 07 08 16 17 18 19 20 21

pons mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 weak expression: see section 06 07 08 16 17 18

midbrain mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 weak expression: see section 05 06 07 08 16 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 05 weak expression: see section 04 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 17 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 weak expression: see section 03 04 06 07 08 09 17 18 19 20 21 22

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 17 18 19

spinal cord mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 weak expression: see section 08

dorsal root ganglion

weak

spotted

weak expression: see section 06 07 08 09 13 14 15 16 17

mandible

moderate

regional

moderate expression: see section 05 06 20 21 weak expression: see section 07 08 09 16 17 18 19

maxilla

moderate

regional

moderate expression: see section 05 weak expression: see section 10 17 18 19

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 22 23 weak expression: see section 01

tail mesenchyme

weak

regional

weak expression: see section 10 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38199
Entity Detected:	Shisa7, shisa homolog 7 (Xenopus laevis) ( MGI:3605641)
Sequence:	sense strand is shown >T38199 ATCTGGATGAAGCCTACCTGAAGCGCAGACAACTGGAGATGCCGCGCGGAACGCTGCCCTTGCATGCACT CCGGCGGCCTGGCACTGGAGGTGGCTACCGTATGGATGGCTGGGGTGGCCCTGAGGAGCTGGGCCTGGCA CCAGCACCCAACCCACGGCGTGTTATGTCCCAGGAGCACCTTCTGGGTGATGGTAGCCGAGCTTCCCGCT ATGAGTTCACGTTGCCTCGAGCGCGCCTGGTGTCTCAGGAACACCTGCTGCTGTCCTCACCTGAGGCGCT TCGCCAGAGTCGCGAGCACCTGCTGTCACCCCCACGAAGTCCTGCACTGCCCCCAGATCCCACCACCCGG GCCAGCCTGGCTGCCTCACACTCCAACCTGCTGCTAGGGCCTGGGGGCCCCCCCACACCCCTGCATGGGT TGCCTCCGTCAGGCCTGCATGCCCACCATCACCATGCCCTTCATGGCTCTCCTCAGCCAGCCTGGATGTC TGATGCGGGCGGGGGTGGGGGCACACTGGCCCGCAGGCCACCCTTCCAGCGCCAGGGCACCCTGGAGCAG CTGCAGTTCATTCCTGGGCACCACCTGCCCCAGCACCTGCGCACTGCCAGCAAGAACGAAGTGACTGTCT GAGGCCCGGTGGAGTGCTGGGGGCTGTGGCTTCCGAGGCCCCCTTCTCCCAGCCCAGATCTCCATTACAA GGCTTATCATACACACCAAGGCTCTGAGACCAGATGGGCCCTTGGACCGGAGACCACGCCTCCTTTTGGG ATGTTACAGTAGATGAGATCTCTCCTTTTACTTTGTCACTGTTTGAACCAAGGCCTCCCTCCCATGATGT CATCCCAGAGGAAGGAGGCCTGTATATGAGGTCATTGCTAGCAAATATGTCTGTGTTTTATGATGTTGTT TGCAGGGACTAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105107. Forward Primer - name:105107_F_cDNA_D430041B17, sequence:ATCTGGATGAAGCCTACCTGAA; Reverse Primer - name:105107_N_SP6_cDNA_D430041B17, sequence:TTAGTCCCTGCAAACAACATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7888 same embryo
	EMAGE:7892 same embryo
	EMAGE:7890 same embryo
	EMAGE:7891 same embryo
	EurExpress:euxassay_012823 same experiment
	MGI:4828060 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source