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EMAGE:7888

Fam154b family with sequence similarity 154, member B ( MGI:1914618)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7888 EMAGE:7888 EMAGE:7888 EMAGE:7888 EMAGE:7888
"Pseudo-wholemount" of euxassay_012801. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012801_01 euxassay_012801_02 euxassay_012801_03 euxassay_012801_04
EMAGE:7888 EMAGE:7888 EMAGE:7888 EMAGE:7888 EMAGE:7888
euxassay_012801_05 euxassay_012801_06 euxassay_012801_07 euxassay_012801_08 euxassay_012801_09
EMAGE:7888 EMAGE:7888 EMAGE:7888 EMAGE:7888 EMAGE:7888
euxassay_012801_10 euxassay_012801_11 euxassay_012801_12 euxassay_012801_13 euxassay_012801_14
EMAGE:7888 EMAGE:7888 EMAGE:7888 EMAGE:7888 EMAGE:7888
euxassay_012801_15 euxassay_012801_16 euxassay_012801_17 euxassay_012801_18 euxassay_012801_19
EMAGE:7888 EMAGE:7888 EMAGE:7888 EMAGE:7888
euxassay_012801_20 euxassay_012801_21 euxassay_012801_22 euxassay_012801_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7888Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7888_wholemount_strong.wlz
7888_wholemount_moderate.wlz
7888_wholemount_possible.wlz
7888_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7888_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
weak weak
regionalweak expression: see section 07 08 09 12 13 16 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38198
Entity Detected:Fam154b, family with sequence similarity 154, member B ( MGI:1914618)
Sequence:sense strand is shown

>T38198
AGTTTCTCACAGAAGGCAAAGCAATTTTCCTTTCCAAGGAAAAAGCACCACGAAGGAAGATTTTCCAGCA
TGGGAAATTTGTCGCCAAGGACTTATTAAGCAGCAGCAACAAATTCCCAACCCATCTGGAAAATTTGAGG
GTTTGAGCACTTTCAGATCTCACTTTGTGCCACATGAATTGATTCCAACAGAGAGCTGCAAACCTTTAAA
TGAAGCTCTTAAGAGTTCAGTTCCACTTGATGATGTCACCATGTATTCTATACAGTTCACACCGAAGAAA
CAGGAAATCTGCCCAGCTAGCTATCCTTCTCCTCCTGGTTATATATTTGAAAATACAAATTCCCAAGGAC
ATAAATTCTTTCGCAAGATTATTCCTGCAGTGAAGGCCTTTTAGTAATCAATGTTTAAAAGAAAGCTAAT
GAGATTTTGATTTTTAAAGAGAAACCCCAACTTTTATAGTAAAAATATGAGAGCTATACAATTTTTAGCT
TTTTGAACAAGTTGGAACATAAAGATCAGAATTGATAAATCCATATATTTTGAACCAAGATCATCCTGTT
TAGCACAGGCTACCCTGGAACTTGCAGTCCTCCTGTGTCAGTCTTCTGATCCAAGATTATTCTTTAACAG
GAATATCTGAAGGCTAAATGATAGGTACGCCCATTTATACATGCTGACTCTTAGTTTGTTGTCACCTCAT
TTTACACTTATGCAATTATACAGCTCCTCTCATC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105682. Forward Primer - name:105682_F_cDNA_D030069K18, sequence:AGTTTCTCACAGAAGGCAAAGC; Reverse Primer - name:105682_N_SP6_cDNA_D030069K18, sequence:GATGAGAGGAGCTGTATAATTGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7892 same embryo
 EMAGE:7890 same embryo
 EMAGE:7891 same embryo
 EMAGE:7889 same embryo
 EurExpress:euxassay_012801 same experiment
 MGI:4824713 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS