EMAGE mouse gene expression spatial database

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EMAGE:7898

Pnmal2 PNMA-like 2 ( MGI:3645856)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012956. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012956_01	euxassay_012956_02	euxassay_012956_03	euxassay_012956_04

euxassay_012956_05	euxassay_012956_06	euxassay_012956_07	euxassay_012956_08	euxassay_012956_09

euxassay_012956_10	euxassay_012956_11	euxassay_012956_12	euxassay_012956_13	euxassay_012956_14

euxassay_012956_15	euxassay_012956_16	euxassay_012956_17	euxassay_012956_18	euxassay_012956_19

euxassay_012956_20	euxassay_012956_21	euxassay_012956_22	euxassay_012956_23	euxassay_012956_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7898_wholemount_strong.wlz

7898_wholemount_moderate.wlz

7898_wholemount_weak.wlz

7898_wholemount_possible.wlz

7898_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7898_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 03 04 19 20 21 22 23 24

upper leg muscle

moderate

regional

moderate expression: see section 01 02 03 04 14 15 16 17 18 19 20 21 22 23 24

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

moderate

regional

moderate expression: see section 24

foot

moderate

regional

moderate expression: see section 04 05 06 19 20 21 22

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 19 20 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

tongue muscle

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38657
Entity Detected:	Pnmal2, PNMA-like 2 ( MGI:3645856)
Sequence:	sense strand is shown >T38657 CCGACAAAGCTAAGAAGGAAGAGATAGAGAAAGACCTTCCTGGGACAGAGTCTATCTGCTTAAACATCAA AGACGAAAAGAGCGGGGTTCCTGATTTAGTCGCGCTACTGGCTGTGAGAGATACCCCCGCAGTGGAGGTA GAGACAGAGGTGGATGATGATGATGAAGATGACGAAGATGAAGATGATGAAGATGATGACGAAGATGAAG ATGACGATGAAGATGATTCCGACAGCGAGTCTCTGGAAAATGGAGAGCACGGAAAGGAAGTGATGGATAG CCCCGAGTTTGTGGCTATTGTGGCTTATACAGACCCCGCTGACCCCTCTGCCAGAGAGGAAATGCTAAAA ATAGCTTCTGTGATTGAGACCCTCGGCTGGGGCGACAAAAAAGACAAAAAAGATGTCCTTCCTCAAGTCC TGTCCGTCATGGCTAAGGACACCTCTGGGCCCAGGGTGAAGGTAGAGGAGGCAGGTCGTCAGGTGGACGC CATGGTCCTGAGGAAGGCAGAGGAAGATGGGAATCTTCTGGAATGTATTTCTACCTTGGCTGAGGCAGAG AACTGCCCTAAGGGAAAGAAGTCTGGGCTAGGTCTCCTCAGAGGCTGGACCGCTGAGGGCCACCAGGGTG GCCTATTGGAGCTGGTGGCACTCTTGGCTGCGCAAGATATGGTGGAAGCTGTGAGAGAAGAAGAAGCCAG CAGGTGGGGCAGTGGTGGAAGTGGTGGCCGGAAGTGTGACCATAGCCAAGGTGGTTTATCTGAGGTCCTG GCTTTCCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108342. Forward Primer - name:108342_F_cDNA_LOC434128, sequence:CCGACAAAGCTAAGAAGGAAGA; Reverse Primer - name:108342_N_SP6_cDNA_LOC434128, sequence:AGGAAAGCCAGGACCTCAGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7899 same embryo
	EMAGE:7902 same embryo
	EMAGE:7901 same embryo
	EMAGE:7897 same embryo
	EMAGE:7900 same embryo
	EurExpress:euxassay_012956 same experiment
	MGI:4827300 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source