EMAGE mouse gene expression spatial database

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EMAGE:8209

Mtbp Mdm2, transformed 3T3 cell double minute p53 binding protein ( MGI:2146005)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005329. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005329_01	euxassay_005329_02	euxassay_005329_03	euxassay_005329_04

euxassay_005329_05	euxassay_005329_06	euxassay_005329_07	euxassay_005329_08	euxassay_005329_09

euxassay_005329_10	euxassay_005329_11	euxassay_005329_12	euxassay_005329_13	euxassay_005329_14

euxassay_005329_15	euxassay_005329_16	euxassay_005329_17	euxassay_005329_18	euxassay_005329_19

euxassay_005329_20	euxassay_005329_21	euxassay_005329_22	euxassay_005329_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8209_wholemount_strong.wlz

8209_wholemount_moderate.wlz

8209_wholemount_weak.wlz

8209_wholemount_possible.wlz

8209_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8209_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 08 09 13 14

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20 21 22

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 12 13 14 15 16 moderate expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7907
Entity Detected:	Mtbp, Mdm2, transformed 3T3 cell double minute p53 binding protein ( MGI:2146005)
Sequence:	sense strand is shown >T7907 TGCTTCCCCGCCACACTCGGGTCCCCGCCGGTAGCGGTTTCGTTATGAGAGGAGCAGAGAGTGTGGCTCC TGAGAGAATAATTTCCCACAATAATTGGCGGGAAAACCGACGTAGGACATTATTTTGCAGCCAACCTGAG CGGCAGGAAACCGGAAGTGCACGTTGCGCGCGGCTCTTTTGAGACCTAACACTGAGTCCGGGCGCGAAGA GGATGGATCGGTACTTGCTGCTGGTCACCTGGAGGGAAGGGAAGTTTCGATCCGTGGCGGGTGGGGAGAT CGAGCCTGGCACTGAGGCGACATCCCTGGAGAGCACCGACAAACAGCCCGATTTGACCGCAACCAATATT TATCACCTCTTGAAGAGAAGCATCAGCGATTCAATCCATCCAGATGACAGTACATTCCCTGCTTGTTCAG TGGGTGGCACACCTCATTCCAGGAAGTGGTTCTTTGCGGTGCAAGCAATATGCGGATTTTACCAGTTTTG TAGTTCTGATTGGCAAGAGATACATTTTGATGCTGAAAAAGATAAAATCGAAGATGTTCTTCAAGCAAAT ATAGAAGAATGTCAGAGTGCTGTTGAGTGTTTTGAAGAAGATGACAGTAATAGCAGGGAATCCTTACCCT TGGCTGACCTATATGAAGAATCAGCAGAAAATTTGCATCAGTTATCAGACAAGCTTCCCGCTCCTGGTAG AGCAATGATAGACATAATACTGTTGCCTTCTGACAAAGACCCTCCTAAGCTAAAAGAGTGCTTGCCCATT GTAGGGGCCTTGAAACATCTGAAGGAATGGCATTCAGCAAAAGTTATCATAGCAGGAAGTTACTGTGAGA TAAATTGTCAGAAAATTGCTGAATACCTTTCAGCTAGTGTTGTGCCTTTAGAAGAATTCAGAAATGCCAT TGATCCGAGGGAACTGTGGCGGGGAGAGATTCAGATGCGGGAACGAAAGTTTGGATTTGAAATTAGTTTT CCTGAATTTTGTTTAAAAGGAGTTACGCCTACGAATGTTAGTGCGTATAATTTAAATACCTGCTTCCTTG CCAAGAAGATAGCATCTTCTAAGGTTTTCCATTATTATGGTCCTGCTTTGGAATTTGTGCAGATGATAAA ACTATCAGATCTTCCCTCCTGTTACATGTCGGATATCGAGTTTGAGTTAGAGGTGACTGGGCACTGCACG AGGCAGAATTCCATGCTGCTGTTGGAACAGATCTCTTCCCTGTGTGGCAAGGTTGGTGCTCTCTTTGTGC TGCCGTGTACTGTTAGCAATGTACTCATCCCACCTCCCAGCCAACTGGCCTCAAGAAAGTGGAAGGAATA CATGGCTAAGAAGCCCAAGACCATCAGTGTTCCCGATGTTGCCGTGAAGGGAGAGTTTTCTGGCTATCAT CTCCTGCTGCAAGGTATGGGCAAGAGAAAATGCAGAGCCACCCTGCTGCACTCGGCCAGCCAGATCAATG GCTCGTTTGCACTCAGTGTCATTCATGGGAAGATGAAAACAAAGGCAGGAGAAGCCAGACCGAGTTTCCC CTTTGACTTCTCGTCACTCCCAAGGTTTTCGGAGGAGCAGGTTTTACAGAGAGAGAAACAATTAGCCAGC TTTCAAGTTTTGGCTTTGAAAGAATGCCTGAAAAGAAGAAAGGCTGCAAACCAGCCCGAAGCCTTTTCTG CCGATGAACTCAAAAGTCTGTTGGCACTCACGAGGGAGCGCTTCCTAGGTCACTTTGATGTTCTCCCCAC TGAAGCAGCTTTAGCACAAACAGACACCGTGAGAGCTGCCGGCGTGGTAAATGATGACGGTACAGTTGAA CCTTATTCTTCAAGCCTAATGGAAACCAATCCTCTGGAATGGCCAGAAAGACATGTTCTTCAGAATTTAG AAACTTCTGAAAAAGCTAAACAAAAAATGAGAACTGGCTCATTACCGCGTTCGTCTGAACAGTTGCTGGG CCATAAAGAGGGTCCCCGGGACTCACTCACATTACTGGATGCTAAGGAGCTGCTGAAGTATTTCACCTCG GATGGGTTACCAGTCGGAGATCTTCAGCCGTTACACATTCAACGGGGGGAAAAGCCTTTTGTTTTGACAC CAGAGCTTAGTCCTGGAAAACTTCAGGTCTTACCTTTTGAAAAAGCCTCGGAATGCCATTACCACGGGAT TGAATATTGCCTGGATGACCAAAAAGCTTTAGAAAGAGATGGGGGATTTTCTGAACTTCAGTCGCGCCTT ATTCGTTACGAGACGCAGACCACCTGCACCAGAGACAGTTTTCCAGTCCCCACCGTGCTGAGCCCTCTTC CATCCCCTGCAGTTCTGTCAGAGCCTCAGAGTGTCCCCGAAGGAGAAGCACTGCAAGGCGAACTGAGGAC TGAAGTTTCTGGATTGAAGCGGAGATCTAAAGACCCCAGCTGCCTGTACCCCCAGAAAAGACTTACGAGA TCAGAAAGTTCTGATTGTTTGCCTTCCCAAGCGAGTTGCAATAGTAATCATCACCATCACACAGGGAAAC CCAGGAAGCCTCAGGCAGAGCGCTGTGTGTCAGGGCTTCCTCTGCCTGGCCGGGAAGCTTCCAAAGATAC CTCAAAGACCAGTTCAGGACAAAAACGAGCACACGAATCAAAATCATCAAAGCAAATGAAGGAATCACGG TCCCAGAAACACACACGGATGCTGAAGGAGGTGGTAAAAGACACCTTGAAGAGGCACCACATCACGGAGG CCCATGAGAGCTTCACGGCCTGCAGCCAGAGACTCTTCGACATCTCCAAGTTCTACCTGAAGGATCTGAA AACATCAAGGGGTCTCTTTGAAGAAATGAAGAAAACAGCCAACAACAATGTGGTGCAGGTGATCGAATGG GTGGTAGAAAAGATGAGCAAGAAATAAGCTACTGCACCCCCTTTCCTTAGAGAACTGTACACAGCCTGTA AACTTTCCCCAAAGAGCAGATGCCTCATTTGTAAGGAGGGTCCATGGTGTCCTGTATCTATATAGTTTTG TGACACTCAGTTATTTTCAACTAATGCACTTATTTTTACAGTTTTACAATATTTTAATCTATGAAATTTT TATATATTTTAAATGTTTTAACTCTTGGTTATTTAATTGTTAAATAAACAGTAATATTTCATTAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6511752 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6511752 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8207 same embryo
	EMAGE:8205 same embryo
	EMAGE:8206 same embryo
	EMAGE:8204 same embryo
	EMAGE:8208 same embryo
	EurExpress:euxassay_005329 same experiment
	MGI:4826487 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source