EMAGE mouse gene expression spatial database

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EMAGE:8282

Atp4b ATPase, H+/K+ exchanging, beta polypeptide ( MGI:88114)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007727. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007727_01	euxassay_007727_02	euxassay_007727_03	euxassay_007727_04

euxassay_007727_05	euxassay_007727_06	euxassay_007727_07	euxassay_007727_08	euxassay_007727_09

euxassay_007727_10	euxassay_007727_11	euxassay_007727_12	euxassay_007727_13	euxassay_007727_14

euxassay_007727_15	euxassay_007727_16	euxassay_007727_17	euxassay_007727_18	euxassay_007727_19

euxassay_007727_20	euxassay_007727_21	euxassay_007727_22	euxassay_007727_23	euxassay_007727_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8282_wholemount_strong.wlz

8282_wholemount_moderate.wlz

8282_wholemount_weak.wlz

8282_wholemount_possible.wlz

8282_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8282_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

stomach

strong

regional

strong expression: see section 06 07 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35959
Entity Detected:	Atp4b, ATPase, H+/K+ exchanging, beta polypeptide ( MGI:88114)
Sequence:	sense strand is shown >T35959 CTACACCCCAGACTACCAGGACCAGTTAAAGTCACCAGGGGTAACCTTGAGACCGGACGTGTATGGGGAA AGAGGGCTGAAGATTTCCTACAATGTCTCTGAAAACAGTTCCTGGGCTGGCCTCACACACACCCTGCACA GCTTCTTAGCAGGCTATACCCCAGCATCCCAGCAGGACAGCATCAACTGTACATCTGAAAAGTACTTCTT CCAGGAGAGCTTTGCAGCTCCAAACCACACCAAGTTCTCCTGCAAGTTCACTGCAGACATGCTACAAAAC TGCTCAGGCCTGGCGGACCCCAGCTTCGGCTTCGAGGAGGGGAAGCCCTGCTTCATTATTAAAATGAACA GAATTGTCAAGTTCCTCCCCAGCAACAACACAGCTCCCCGAGTGGACTGCACCTTCCAGGATGATCCCCA AAAGCCCCGGAAGGACACTGAACCCCTGCAGGTCGAGTACTATCCCCCCAATGGCACCTTCAGTCTCCAC TATTTCCCCTACTATGGCAAGAAAGCACAGCCCCACTACAGCAACCCTCTGGTGGCAGCCAAGCTCCTCA ACGTCCCCAAGAACATGCAAGTCAGCATCGTGTGCAAGATCCTGGCAGACCACGTGACCTTCAACAACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89751. Forward Primer - name:089751_F_cDNA_Atp4b, sequence:CTACACCCCAGACTACCAGGAC; Reverse Primer - name:089751_N_SP6_cDNA_Atp4b, sequence:GGTTGTTGAAGGTCACGTGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8285 same embryo
	EMAGE:8283 same embryo
	EMAGE:8286 same embryo
	EMAGE:8284 same embryo
	EurExpress:euxassay_007727 same experiment
	MGI:4823312 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source