EMAGE mouse gene expression spatial database

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EMAGE:8285

Angptl2 angiopoietin-like 2 ( MGI:1347002)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007716. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007716_01	euxassay_007716_02	euxassay_007716_03	euxassay_007716_04

euxassay_007716_05	euxassay_007716_06	euxassay_007716_07	euxassay_007716_08	euxassay_007716_09

euxassay_007716_10	euxassay_007716_11	euxassay_007716_12	euxassay_007716_13	euxassay_007716_14

euxassay_007716_15	euxassay_007716_16	euxassay_007716_17	euxassay_007716_18	euxassay_007716_19

euxassay_007716_20	euxassay_007716_21	euxassay_007716_22	euxassay_007716_23	euxassay_007716_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8285_wholemount_strong.wlz

8285_wholemount_moderate.wlz

8285_wholemount_weak.wlz

8285_wholemount_possible.wlz

8285_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8285_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

diaphragm

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

upper arm mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 19 20 21 22 23 24

hand

strong

regional

strong expression: see section 01 02 24

foot

strong

regional

strong expression: see section 05 06 07 08 24

lower leg mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06

upper leg mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 10 21 22 23 24

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon meninges

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spinal cord meninges

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

aorta

strong

regional

strong expression: see section 11 12 13 14 15

heart valve

strong

regional

strong expression: see section 10 11 12 13 14 15

mandible

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

maxilla

strong

regional

strong expression: see section 09 10 11 17 18 19 20

axial skeleton

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

basioccipital bone

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 18 19 20 21 22 23 24

vault of skull

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07

clavicle

strong

regional

strong expression: see section 07 08 09 10 15 16 17 18 19

sternum

strong

regional

strong expression: see section 13 14

pelvic girdle skeleton

strong

regional

strong expression: see section 11 12 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35930
Entity Detected:	Angptl2, angiopoietin-like 2 ( MGI:1347002)
Sequence:	sense strand is shown >T35930 TACCAGTCTCCCATCTTCCACTGATAAGCCATCAGGTCCATGGAGAGACTGCCTGCAAGCCCTGGAAGAT GGTCACAGCACCAGCTCCATCTACCTGGTGAAGCCTGAGAATACCAACCGCCTGATGCAGGTGTGGTGTG ACCAGAGACATGACCCTGGAGGTTGGACTGTCATCCAGAGACGCCTGGATGGCTCTGTCAACTTCTTCAG GAACTGGGAGACCTATAAGCAAGGGTTTGGGAACATCGATGGTGAGTACTGGCTGGGCCTGGAGAACATC TACTGGCTGACGAACCAAGGCAACTACAAACTGCTGGTAACCATGGAGGACTGGTCTGGCCGTAAAGTCT TTGCTGAGTATGCCAGTTTCCGACTGGAGCCAGAAAGCGAGTACTATAAGCTGCGGCTGGGGCGTTATCA TGGCAATGCAGGCGACTCCTTTACCTGGCACAACGGCAAACAGTTTACCACCCTGGACAGGGACCATGAT GTCTACACAGGAAACTGTGCCCACTATCAGAAGGGAGGATGGTGGTATAACGCCTGTGCTCACTCCAACC TCAATGGGGTCTGGTACCGTGGGGGCCATTACCGGAGCAGATACCAGGACGGGGTCTACTGGGCTGAGTT CCGAGGAGGCTCTTACTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94794. Forward Primer - name:094794_F_cDNA_Angptl2, sequence:TACCAGTCTCCCATCTTCCACT; Reverse Primer - name:094794_N_SP6_cDNA_Angptl2, sequence:GAGTAAGAGCCTCCTCGGAACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8283 same embryo
	EMAGE:8286 same embryo
	EMAGE:8282 same embryo
	EMAGE:8284 same embryo
	EurExpress:euxassay_007716 same experiment
	MGI:4823124 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source