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EMAGE:8348

Pgrmc2 progesterone receptor membrane component 2 ( MGI:1918054)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8348 EMAGE:8348 EMAGE:8348 EMAGE:8348 EMAGE:8348
"Pseudo-wholemount" of euxassay_007705. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007705_01 euxassay_007705_02 euxassay_007705_03 euxassay_007705_04
EMAGE:8348 EMAGE:8348 EMAGE:8348 EMAGE:8348 EMAGE:8348
euxassay_007705_05 euxassay_007705_06 euxassay_007705_07 euxassay_007705_08 euxassay_007705_09
EMAGE:8348 EMAGE:8348 EMAGE:8348 EMAGE:8348 EMAGE:8348
euxassay_007705_10 euxassay_007705_11 euxassay_007705_12 euxassay_007705_13 euxassay_007705_14
EMAGE:8348 EMAGE:8348 EMAGE:8348 EMAGE:8348 EMAGE:8348
euxassay_007705_15 euxassay_007705_16 euxassay_007705_17 euxassay_007705_18 euxassay_007705_19
EMAGE:8348 EMAGE:8348
euxassay_007705_20 euxassay_007705_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8348Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8348_wholemount_strong.wlz
8348_wholemount_moderate.wlz
8348_wholemount_weak.wlz
8348_wholemount_possible.wlz
8348_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8348_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
strong strong
regionalstrong expression: see section 06 07 08 09 15 16 17 18 19
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 10 11 16 17
pons mantle layer
moderate moderate
regionalmoderate expression: see section 09 18
facial vii ganglion
weak weak
regionalweak expression: see section 04
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 17 18 19 20 weak expression: see section 04 05 06 07 08 16
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 19 weak expression: see section 08 09
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 03 21 moderate expression: see section 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36778
Entity Detected:Pgrmc2, progesterone receptor membrane component 2 ( MGI:1918054)
Sequence:sense strand is shown

>T36778
GAATATCCAGCGGATTGAAGTCAGCAGTTCCCAGCAACGATAATTTGAAACCAGTTTTATTTCTTTCCCT
TCCTTCCGCTAGGTCAAATCAATATTAATTGTGCCTTATTTCACTTCCATAAACTTGAATTACTTTTTGG
GAAAGCCCCTCTGTGAATTGAGAAGTCACTACAAGCAGCATTGAGCCTGAGGCTGATGTCATTCTGTGTG
TAGAATGGAAGAGGAGTACTCAGTGTTAACTGCCATATGTTAGAACACAAACATAGCTAGCATTGTGATG
GCCAAATGCATCCCCTGGGCTCTTTTCTTTTCCAAGAAAAATTGAAAAACCAACTACTCTTCCCCAACAG
CTGCCTAATTTTAGGAGTCTGACCCTCACTCGTCACTGGTGTGGGTAGCAGGGCCGTGGTATGAATGTCT
GTGTGAGTGAGTCTGGATGTCTGTGAGAGCACCTTGGGGGAAACTTCCTGCGATGCTGTAAATACGAGTA
CCGTTTTAATAAAGCATGTACAATAAACCAAATAAGCTCGAGTCGGACTCTAAATACAGAGCCGTGAGCC
AGTGTGTCATGTACTAATACTGCATTTGATTCACGATGAGGGGGAAACCCTCTTAGAAATGAAAATCAGG
CCCTGACTAGCCAAGCTGTCCGTGTCCCATGCTCAGTGTAACTTCCAAGGCAGGTCCACCTCTGTGGGTG
GTTCAGGTAAGAAGGGATATTACAGTGGACAGTGCTCTCAGGCCTCGCTGCCACTCAGTGTGCTGGAAGC
ACCAGCCTTCCAAAGTAGGAACTGACATAGAAACACAGTATTGTACCCATAGCCAGTAAATCCCGTTCTT
GTCAGTTAAAAAAGTTCTATTTCAAAGCCTGCTTAGTTTGGGTTTTGGCTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77172. Forward Primer - name:077172_F_cDNA_Pgrmc2, sequence:GAATATCCAGCGGATTGAAGTC; Reverse Primer - name:077172_N_SP6_cDNA_Pgrmc2, sequence:GAAGCCAAAACCCAAACTAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8347 same embryo
 EMAGE:8349 same embryo
 EMAGE:8351 same embryo
 EMAGE:8350 same embryo
 EurExpress:euxassay_007705 same experiment
 MGI:4827180 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS