EMAGE mouse gene expression spatial database

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EMAGE:8411

Mmp23 matrix metallopeptidase 23 ( MGI:1347361)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007272. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007272_01	euxassay_007272_02	euxassay_007272_03	euxassay_007272_04

euxassay_007272_05	euxassay_007272_06	euxassay_007272_07	euxassay_007272_08	euxassay_007272_09

euxassay_007272_10	euxassay_007272_11	euxassay_007272_12	euxassay_007272_13	euxassay_007272_14

euxassay_007272_15	euxassay_007272_16	euxassay_007272_17	euxassay_007272_18	euxassay_007272_19

euxassay_007272_20	euxassay_007272_21	euxassay_007272_22	euxassay_007272_23	euxassay_007272_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8411_wholemount_strong.wlz

8411_wholemount_moderate.wlz

8411_wholemount_weak.wlz

8411_wholemount_possible.wlz

8411_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8411_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardial cavity

moderate

regional

moderate expression: see section 04 05 06 07 08 09 12 13 15 16 17 18 19 20 21 22 23 weak expression: see section 14

bladder

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 12

kidney calyx

weak

regional

weak expression: see section 07 08 09 10 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1886
Entity Detected:	Mmp23, matrix metallopeptidase 23 ( MGI:1347361)
Sequence:	sense strand is shown >T1886 TGGCCTCGAGCCAGATTCGGCACGAGGCCACAGGTGAACTGGCCCACGCTTTCTTCCCACCCCACGGTGG CATTCACTTTGATGACAGCGAGTACTGGGTCTTGGGCCCCACACGCTACAGTTGGAAGAAAGGTGTTTGG CTCACAAACCTGGTGCACGTGGCAGCCCATGAGATTGGCCATGCACTGGGCCTGATGCACTCACAGCAAG ATCAGGCGCTCATGCACCTCAATGCCACATTGCGAGGCTGGAAGGCACTGTCCCAGGATGAACTGTGGGG GTTACACCGACTCTATGGCTGCCTGGACCGGATTTTTGTGTGTGCATCCTGGGCACGAAAGGGATTTTGT GATGTCCGCCAGAGGCTCATGAAGAGGCTCTGCCCCAGAAGCTGTGATTTCTGTTATGAATTCCCATTTC CCACGGTGGCCACCACCACATCACCCACCAGAACGAAAACAAGGTTGGTGAGAGAGGGTAGGAACATGAC CTTCCACTGTGGACAGAAGATCCTACACAAGAAAGGGAAAGTATACTGGTACAAGGA
Notes:	The probe template was PCR amplified from IMAGE:597664 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:597664 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8415 same embryo
	EMAGE:8414 same embryo
	EMAGE:8413 same embryo
	EMAGE:8416 same embryo
	EMAGE:8412 same embryo
	EurExpress:euxassay_007272 same experiment
	MGI:4826409 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source