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EMAGE:8546

Neurog2 neurogenin 2 ( MGI:109619)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8546 EMAGE:8546 EMAGE:8546 EMAGE:8546 EMAGE:8546
"Pseudo-wholemount" of euxassay_009805. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009805_01 euxassay_009805_02 euxassay_009805_03 euxassay_009805_04
EMAGE:8546 EMAGE:8546 EMAGE:8546 EMAGE:8546 EMAGE:8546
euxassay_009805_05 euxassay_009805_06 euxassay_009805_07 euxassay_009805_08 euxassay_009805_09
EMAGE:8546 EMAGE:8546 EMAGE:8546 EMAGE:8546 EMAGE:8546
euxassay_009805_10 euxassay_009805_11 euxassay_009805_12 euxassay_009805_13 euxassay_009805_14
EMAGE:8546 EMAGE:8546 EMAGE:8546 EMAGE:8546 EMAGE:8546
euxassay_009805_15 euxassay_009805_16 euxassay_009805_17 euxassay_009805_18 euxassay_009805_19
EMAGE:8546 EMAGE:8546
euxassay_009805_20 euxassay_009805_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8546Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8546_wholemount_strong.wlz
8546_wholemount_moderate.wlz
8546_wholemount_weak.wlz
8546_wholemount_possible.wlz
8546_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8546_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hypothalamus ventricular layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 12
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 08 09 10 11 12
olfactory cortex ventricular layer
strong strong
regionalstrong expression: see section 07 08 11 12
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 01
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30658
Entity Detected:Neurog2, neurogenin 2 ( MGI:109619)
Sequence:sense strand is shown

>T30658
GCCCTTCTCCACCTTCCTCCTGGAGCTGCACCAACAGCCCGGCGTCATCCTCCAACTCCACGTCCCCATA
CAGCTGCACTTTATCGCCCGCTAGCCCCGGGTCAGACGTGGACTACTGGCAGCCCCCACCTCCGGAGAAG
CATCGTTATGCGCCTCACCTGCCCCTCGCCAGGGACTGTATCTAGAGCTGCGGGTCTCCCTCTCTCGTCC
TCTACCCGGCCCTCTTCCCATCCTTCTCCCGCCCCTCACCCTCCACGCCCCGGACTCCACTTCACAGAGC
AGAGGTGGCCCTTGCAATCCCCTCGGCGGCTGGTGCATTCGGGGGTGGAGACCAGCTCTGGTTTATTGAA
GATGTGAGGATTTATGGTCAAAGAGGACTATGGCGTGTGGGAGTGGGGGCTGGCGTGGGGAACCTCGTAA
GACTGTAAAAGACACTGAGAAAAAGTACCATAACTAACGAGTGTGCAGAGCAGACTGACGCTCCTCCCCT
CTCTCAGAGCTGCTGGAGGAGAACTCCGGGCAGGCAGTTCGTGTGAATCTCTCAGAGGGAATGCAACTGG
TCCCTGTGATCTTTTCACCTTCGTTTCTACATAGAGATGTTAATGTCAGTCGAAAGAAATGTATTTTAGC
ATCTGAATGAATTTACTGGTAATAATATTATCCACACATTTGCAATGGCTGGCATCTGCTCTATTCCCAT
TGCTGTCTGCAGGCTGTGGGAATTTCACCTGTCAAACCAAACTTTCCCTCTCTGATGTGCACTTTGTTTT
TTTCCCAGATTCGTCACAATGCCTATTGTCCCGCCCTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6808442), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59739. Forward Primer - name:059739_F_IRAV117_a01_Neurog2, sequence:GCCCTTCTCCACCTTCCT; Reverse Primer - name:059739_R_SP6_IRAV117_a01_Neurog2, sequence:GAAGGGCGGGACAATAGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8548 same embryo
 EMAGE:8547 same embryo
 EMAGE:8545 same embryo
 EMAGE:8550 same embryo
 EMAGE:8549 same embryo
 EurExpress:euxassay_009805 same experiment
 MGI:4826677 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS