Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:8547

Dclk2 doublecortin-like kinase 2 ( MGI:1918012)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8547 EMAGE:8547 EMAGE:8547 EMAGE:8547 EMAGE:8547
"Pseudo-wholemount" of euxassay_009796. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009796_01 euxassay_009796_02 euxassay_009796_03 euxassay_009796_04
EMAGE:8547 EMAGE:8547 EMAGE:8547 EMAGE:8547 EMAGE:8547
euxassay_009796_05 euxassay_009796_06 euxassay_009796_07 euxassay_009796_08 euxassay_009796_09
EMAGE:8547 EMAGE:8547 EMAGE:8547 EMAGE:8547 EMAGE:8547
euxassay_009796_10 euxassay_009796_11 euxassay_009796_12 euxassay_009796_13 euxassay_009796_14
EMAGE:8547 EMAGE:8547 EMAGE:8547 EMAGE:8547 EMAGE:8547
euxassay_009796_15 euxassay_009796_16 euxassay_009796_17 euxassay_009796_18 euxassay_009796_19
EMAGE:8547 EMAGE:8547 EMAGE:8547 EMAGE:8547 EMAGE:8547
euxassay_009796_20 euxassay_009796_21 euxassay_009796_22 euxassay_009796_23 euxassay_009796_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8547Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8547_wholemount_strong.wlz
8547_wholemount_moderate.wlz
8547_wholemount_weak.wlz
8547_wholemount_possible.wlz
8547_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8547_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 01 02 03 04 05 06 07 08
facial vii ganglion
weak weak
regionalweak expression: see section 03 04 05 06 18 19 20 21
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 07 18
trigeminal v ganglion
weak weak
regionalweak expression: see section 02 03 04 05 06 07 16 17 18 19 20 21 22
vagus x ganglion
weak weak
regionalweak expression: see section 06 07 17
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 06 07 17 18 19
trigeminal v nerve
weak weak
regionalweak expression: see section 16
spinal cord
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 weak expression: see section 08
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 09 15
cervical ganglion
weak weak
regionalweak expression: see section 08 16
thoracic ganglion
weak weak
regionalweak expression: see section 10 11 12 13
dorsal root ganglion
weak weak
regionalweak expression: see section 06 07 08 09 10 13 14 15 16 17
middle ear mesenchyme
weak weak
regionalweak expression: see section 01 02 03 22 23 24
neural retina
weak weak
regionalweak expression: see section 01 02 21 22 23 24
nasal cavity
weak weak
regionalweak expression: see section 09 10 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30659
Entity Detected:Dclk2, doublecortin-like kinase 2 ( MGI:1918012)
Sequence:sense strand is shown

>T30659
TGGGGACCGCTATTTCAAAGGCCTGGTCTTTGCCATCTCCAACGACCGTTTCCGTTCCTTCGATGCGCTC
CTCATAGAGCTCACCCGCTCACTGTCTGACAATGTGAACCTGCCCCAGGGCGTCCGCACCATCTACACCA
TAGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAAGGTGAAAGTTACGTGTGTGCCTCCAA
CGAACCATTTCGTAAAGTTGATTACACCAAAAACGTTAATCCGAACTGGTCTGTGAACATCAAGGGCGGA
ACCACCCGCACCCTGGCCGTGGCCTCTGCGAAGAGTGAGGTGAAAGAAAGCAAAGACTTCATTAAACCCA
AGTTAGTGACTGTGATTCGAAGCGGAGTGAAGCCTAGAAAGGCCGTGCGGATCCTTCTGAATAAAAAGAC
TGCCCATTCCTTTGAGCAGGTCTTAACAGATATCACCGAAGCCATTAAACTAGACTCAGGCGTGGTCAAG
AGGCTGTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGACTTTTTTGGAGACGATGATGTTTTTA
TTGCATGTGGACCTGAAAAATATCGTTATGCCCAAGATGACTTTGTCCTGGATCATAGCGAATGCCGTGT
CCTGAAATCGTCCTACTCTCGAGCCTCAGCTGCGAAGTATTCTGGATCCAGAAGCCCAGGGTTCTCCCGC
CGCAGCAAGTCACCAGCTTCAGTGAATGGAACTCCCAGTAGCCAGCTTTCCACTCCGAAGTCCACCAAGT
CCTCCAGCTCCTCTCCAACCAGCCCGGGAAGTTTCAGAGGATTGAAGCAGATTTCTGCTCAGGGCAGATC
TTCTTCCAACGTAAACGGTGGGCCTGAACTTGACCGTTGCCTGAGCCCTGAAGGTGTGAATGGAAACCGG
TGCTCCGAGTCGTTCCCCCTTCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6822681), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59455. Forward Primer - name:059455_F_IRAV117_a03_6330415M09Rik, sequence:TGGGGACCGCTATTTCAA; Reverse Primer - name:059455_R_SP6_IRAV117_a03_6330415M09Rik, sequence:CCAGAAGGGGGAACGACT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8548 same embryo
 EMAGE:8546 same embryo
 EMAGE:8545 same embryo
 EMAGE:8550 same embryo
 EMAGE:8549 same embryo
 EurExpress:euxassay_009796 same experiment
 MGI:4824250 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS