EMAGE mouse gene expression spatial database

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EMAGE:8565

Ptprs protein tyrosine phosphatase, receptor type, S ( MGI:97815)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009779. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009779_01	euxassay_009779_02	euxassay_009779_03	euxassay_009779_04

euxassay_009779_05	euxassay_009779_06	euxassay_009779_07	euxassay_009779_08	euxassay_009779_09

euxassay_009779_10	euxassay_009779_11	euxassay_009779_12	euxassay_009779_13	euxassay_009779_14

euxassay_009779_15	euxassay_009779_16	euxassay_009779_17	euxassay_009779_18	euxassay_009779_19

euxassay_009779_20	euxassay_009779_21	euxassay_009779_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8565_wholemount_strong.wlz

8565_wholemount_moderate.wlz

8565_wholemount_weak.wlz

8565_wholemount_possible.wlz

8565_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8565_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

strong

regional

strong expression: see section 05 06 17 moderate expression: see section 02 04 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 17 moderate expression: see section 16

trigeminal v ganglion

strong

regional

strong expression: see section 01 05 06 17 moderate expression: see section 02 03 04 07 16 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 06 moderate expression: see section 07 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 17 moderate expression: see section 16

trigeminal v nerve

strong

regional

strong expression: see section 08 09 15 16

spinal cord

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 14 15

cervical ganglion

moderate

regional

moderate expression: see section 07 08 15 16

thoracic ganglion

moderate

regional

moderate expression: see section 10 11

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 11 12 13 14 15 16

neural retina

moderate

regional

moderate expression: see section 01 02 03 22

nasal cavity olfactory epithelium

strong

single cell

strong expression: see section 09 10 11 12 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36926
Entity Detected:	Ptprs, protein tyrosine phosphatase, receptor type, S ( MGI:97815)
Sequence:	sense strand is shown >T36926 CCTTCCTCATTCCCTTCTGATTCCAAAACGAGGTTCCAGGGTGGGGGGTTGGGGTGGAGAGAGAAGGAGC CACTGCTCCCCAGGCTGGGGTCACACAGGGACCGACCTCTGCTTCCGCACTCCCCTGCCTGCCTTTTGGC AACATTTTTTTTCTTATTTTTTTTTAATAGTGTATATTTTTTTTCTTTTTCTTTTTTTCTTTTTTTTTTT TAAGAAAAAAACAAAATCGTGCCGGTCAAAACTTTGAAAAAGAAACAAGATCACTGTTTGTGCCTCTGTG GGAGGCCTATTTTTTCATAGTTAGTGTGCCGTGTGGCGGCTATGTGCGGCCACTTCGACGGCTTCTGTGT GTGCATCTTTCCCACATGCCCGACACTGCCCCCATCCCCATGTGAATGGTGCGCTTAGTTTTTATTTTTA ACCTTTTTACTTTTTTTTTAATCAATCTTCAGACATATCAGATATGGAGGGTGAGGCGCTGGGGGCACTC GGGCCAGACTACAGGGACATGGCCACCAAGGACACAGTGGCTGGCCTTGCTGCTCCCAGTCCCTGGCACA CCAGGGAGGGTCCTCGTCTACTCATGACCTCTGTGCCCCGCATGGAGGACCTGGGACTACGGGACACTTG GGGGATATCCAACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64185. Forward Primer - name:064185_F_cDNA_Ptprs, sequence:CCTTCCTCATTCCCTTCTGATT; Reverse Primer - name:064185_N_SP6_cDNA_Ptprs, sequence:GGTTGGATATCCCCCAAGTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8570 same embryo
	EMAGE:8567 same embryo
	EMAGE:8566 same embryo
	EMAGE:8569 same embryo
	EMAGE:8568 same embryo
	EurExpress:euxassay_009779 same experiment
	MGI:4827533 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source