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EMAGE:8568

Celf3 CUGBP, Elav-like family member 3 ( MGI:1926034)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8568 EMAGE:8568 EMAGE:8568 EMAGE:8568 EMAGE:8568
"Pseudo-wholemount" of euxassay_009785. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009785_01 euxassay_009785_02 euxassay_009785_03 euxassay_009785_04
EMAGE:8568 EMAGE:8568 EMAGE:8568 EMAGE:8568 EMAGE:8568
euxassay_009785_05 euxassay_009785_06 euxassay_009785_07 euxassay_009785_08 euxassay_009785_09
EMAGE:8568 EMAGE:8568 EMAGE:8568 EMAGE:8568 EMAGE:8568
euxassay_009785_10 euxassay_009785_11 euxassay_009785_12 euxassay_009785_13 euxassay_009785_14
EMAGE:8568 EMAGE:8568 EMAGE:8568 EMAGE:8568 EMAGE:8568
euxassay_009785_15 euxassay_009785_16 euxassay_009785_17 euxassay_009785_18 euxassay_009785_19
EMAGE:8568 EMAGE:8568 EMAGE:8568
euxassay_009785_20 euxassay_009785_21 euxassay_009785_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8568Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8568_wholemount_strong.wlz
8568_wholemount_moderate.wlz
8568_wholemount_weak.wlz
8568_wholemount_possible.wlz
8568_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8568_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
adrenal medulla
strong strong
spottedstrong expression: see section 05 06 07 12 13 14
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
facial vii ganglion
strong strong
regionalstrong expression: see section 02 03 04 05 17 18 19 20
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 05 17
trigeminal v ganglion
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20
vagus x ganglion
strong strong
regionalstrong expression: see section 06 15 16
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 04 05 06 16 17
trigeminal v nerve
strong strong
regionalstrong expression: see section 08 15
spinal cord
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15
cervico-thoracic ganglion
strong strong
spottedstrong expression: see section 08 12 13 14
cervical ganglion
strong strong
spottedstrong expression: see section 07 08 15 16
thoracic ganglion
strong strong
spottedstrong expression: see section 08 09 10 11 12
dorsal root ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 10 11 13 14 moderate expression: see section 12 15 16
neural retina
strong strong
regionalstrong expression: see section 01 02 03 21 22
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17
vomeronasal organ
strong strong
regionalstrong expression: see section 11 14
esophagus
strong strong
spottedstrong expression: see section 10 11
tongue
strong strong
spottedstrong expression: see section 09 10 11 13 14 15
stomach
strong strong
spottedstrong expression: see section 01 02 03 04 05 06 07
midgut
strong strong
spottedstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30634
Entity Detected:Celf3, CUGBP, Elav-like family member 3 ( MGI:1926034)
Sequence:sense strand is shown

>T30634
GACGGTACCAGCAAAGGCTGTGCCTTTGTGAAGTTCCAGACTCACGCTGAGGCCCAGGCAGCCATCAACA
CCCTTCACAGCAGCCGGACCCTACCGGGTGCCTCATCCAGCCTGGTGGTAAAGTTTGCTGACACGGAGAA
GGAGCGAGGTCTCCGTCGAATGCAGCAGGTGGCTACCCAGCTGGGCATGTTCAGCCCGATCGCCCTCCAG
TTTGGAGCCTACAGCGCCTACACCCAGGCCCTGATGCAGCAGCAGGCGGCCCTGGTAGCAGCTCACAGTG
CCTACCTCAGCCCTATGGCCACCATGGCTGCCGTGCAGATGCAGCACATGGCTGCCATCAGTGCCAATGG
CCTCATCGCCACCCCCATCACTCCATCCTCAGGAACCAGCACCCCTCCTGCCATTGCTGCCACGCCCGTC
TCTGCCATCCCTGCTGCCTTGGGCGTCAACGGCTACAGCCCGGTGCCCACCCAGCCTACAGGGCAGCCTG
CCCCGGATGCTCTGTATCCCAACGGGGTTCACCCTTAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6401356), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59515. Forward Primer - name:059515_F_IRAV115_h04_Tnrc4, sequence:GACGGTACCAGCAAAGGC; Reverse Primer - name:059515_R_SP6_IRAV115_h04_Tnrc4, sequence:GGTAAGGGTGAACCCCGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8570 same embryo
 EMAGE:8567 same embryo
 EMAGE:8566 same embryo
 EMAGE:8565 same embryo
 EMAGE:8569 same embryo
 EurExpress:euxassay_009785 same experiment
 MGI:4828863 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS