EMAGE mouse gene expression spatial database

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EMAGE:8840

Rgs2 regulator of G-protein signaling 2 ( MGI:1098271)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012997. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012997_01	euxassay_012997_02	euxassay_012997_03	euxassay_012997_04

euxassay_012997_05	euxassay_012997_06	euxassay_012997_07	euxassay_012997_08	euxassay_012997_09

euxassay_012997_10	euxassay_012997_11	euxassay_012997_12	euxassay_012997_13	euxassay_012997_14

euxassay_012997_15	euxassay_012997_16	euxassay_012997_17	euxassay_012997_18	euxassay_012997_19

euxassay_012997_20	euxassay_012997_21	euxassay_012997_22	euxassay_012997_23	euxassay_012997_24

euxassay_012997_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8840_wholemount_strong.wlz

8840_wholemount_moderate.wlz

8840_wholemount_weak.wlz

8840_wholemount_possible.wlz

8840_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8840_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 07 08 09 15 16 17

pituitary gland

strong

regional

strong expression: see section 11 12 13 14 15 16 17

pineal primordium

strong

regional

strong expression: see section 13

olfactory cortex mantle layer

strong

regional

strong expression: see section 12 moderate expression: see section 16

medulla oblongata floor plate

strong

regional

strong expression: see section 13

metencephalon floor plate

strong

regional

strong expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38863
Entity Detected:	Rgs2, regulator of G-protein signaling 2 ( MGI:1098271)
Sequence:	sense strand is shown >T38863 AGGAGAGGCTCTGTGTGAGAACGGCTTCCCTCACTGTGTGAAGAACAGAGGGAGGGAACAGGCCTCTGAA TGTGTTCTTCCTCCTTGTCGGGAAAGCAGAGTTTGAGATGAAAGATCCGATGCAATGTTGTTGGAGCATT TAAAATCAATAGGTCTGGGATTATGTGGCCTTAGCTAGTTGGCTGTACACCTTCCCTAAACTAGTCCATG TTACCACATAGTGGTGTTAGTTCTAGTTTTAATATTTTAGTACTAAGTAACATTACAATGTTTACTGTGT GCAAGGGTGTTGACGTTCTTAGGACTACAGATCATTAGTACTAGTGTGTCACGTATCACTGAAACTGAGA AGTATGTTTGAGTTGTTAAATGGTGTGTGTGATGGACCGAATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 177431. Forward Primer - name:177431_F_cDNA_Rgs2, sequence:AGGAGAGGCTCTGTGTGAGAAC; Reverse Primer - name:177431_N_SP6_cDNA_Rgs2, sequence:CATTCGGTCCATCACACACAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8839 same embryo
	EMAGE:8842 same embryo
	EMAGE:8841 same embryo
	EMAGE:8838 same embryo
	EMAGE:8837 same embryo
	EurExpress:euxassay_012997 same experiment
	MGI:4827719 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source