EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:8876

Rab21 RAB21, member RAS oncogene family ( MGI:894308)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010091. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010091_01	euxassay_010091_02	euxassay_010091_03	euxassay_010091_04

euxassay_010091_05	euxassay_010091_06	euxassay_010091_07	euxassay_010091_08	euxassay_010091_09

euxassay_010091_10	euxassay_010091_11	euxassay_010091_12	euxassay_010091_13	euxassay_010091_14

euxassay_010091_15	euxassay_010091_16	euxassay_010091_17	euxassay_010091_18	euxassay_010091_19

euxassay_010091_20	euxassay_010091_21	euxassay_010091_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8876_wholemount_strong.wlz

8876_wholemount_moderate.wlz

8876_wholemount_weak.wlz

8876_wholemount_possible.wlz

8876_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8876_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 09 10 11 12 13

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 15 16

facial vii ganglion

weak

regional

weak expression: see section 03 04 19 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 17

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 15 16 17 18 19 20 21

vagus x ganglion

weak

regional

weak expression: see section 06 16

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 09 13 14 15 16

neural retina

weak

regional

weak expression: see section 01 02 21 22

mandible

weak

regional

weak expression: see section 01 02 03 19 20 21

maxilla

moderate

regional

moderate expression: see section 09 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T32128
Entity Detected:	Rab21, RAB21, member RAS oncogene family ( MGI:894308)
Sequence:	sense strand is shown >T32128 GAAGACGTCGCTGGTGCTGCGCTACTGCGAGAACAAGTTCAACGACAAGCACATCACCACCCTGCAGGCA TCTTTCTTAACAAAGAAGTTAAATATTGGTGGAAAAAGAGTAAACCTTGCCATATGGGACACAGCAGGTC AGGAGAGATTCCACGCACTGGGCCCAATTTACTACCGAGATTCGAACGGAGCTATTTTAGTTTATGATGT AACGGATGAAGATTCCTTTCAGAAGGTAAAAAACTGGGTCAAAGAATTACGAAAAATGTTGGGAAATGAG ATCTGTTTGTGTATAGTTGGTAATAAAATAGACTTGGAAAAGGAGAGGCACGTCTCTATTCAAGAAGCAG AATCGTATGCAGAGTCTGTGGGAGCCAAGCATTACCATACTTCCGCTAAACAGAACAAAGGCATTGAGGA GCTCTTTCTCGACCTTTGTAAAAGAATGATAGAGACAGCCCAGGTGGATGAGAGAGCAAAAGGCAATGGC TCGAGTCAAGCCGGGGCTGCAAGGCGAGGTGTGCAGATCATCGACGACGAGCCTCAAGCTCAGAGCAGCG GAGGGTGCTGTTCCTCCGGCTAACTGCTCAGCCTGTGACCCTCAAAACATAAGAGAACTGTGGATCATTG CCCTCAACATGAAGACTGCCATATTCCAAGTCACATCATTTTACCAATGGAGTTATAGAATTAACAGTAT TTTCAATTACGTTTATAACACTGCAGAGACCTTAAGTGCTAAACTTAGTGGAGTTTGTGACCAGAGAATT GGCATTTTCTACAAATGTTAACAAAGCAATTACCAATGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6490069), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59268. Forward Primer - name:059268_F_IRAV94_d08_Rab21, sequence:GAAGACGTCGCTGGTGCT; Reverse Primer - name:059268_R_SP6_IRAV94_d08_Rab21, sequence:GGCCATTGGTAATTGCTTTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8878 same embryo
	EMAGE:8873 same embryo
	EMAGE:8875 same embryo
	EMAGE:8877 same embryo
	EMAGE:8874 same embryo
	EurExpress:euxassay_010091 same experiment
	MGI:4827567 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source