EMAGE mouse gene expression spatial database

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EMAGE:8887

Kctd20 potassium channel tetramerisation domain containing 20 ( MGI:1914239)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010084. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010084_01	euxassay_010084_02	euxassay_010084_03	euxassay_010084_05

euxassay_010084_06	euxassay_010084_07	euxassay_010084_08	euxassay_010084_09	euxassay_010084_10

euxassay_010084_11	euxassay_010084_12	euxassay_010084_13	euxassay_010084_14	euxassay_010084_15

euxassay_010084_16	euxassay_010084_17	euxassay_010084_18	euxassay_010084_19	euxassay_010084_20

euxassay_010084_21	euxassay_010084_22	euxassay_010084_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8887_wholemount_strong.wlz

8887_wholemount_moderate.wlz

8887_wholemount_weak.wlz

8887_wholemount_possible.wlz

8887_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8887_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 07 08 14 15

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 06 17 18

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 16 17 18 19 20 21

vagus x ganglion

weak

regional

weak expression: see section 07 16

dorsal root ganglion

weak

regional

weak expression: see section 05 06 07 08 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T32086
Entity Detected:	Kctd20, potassium channel tetramerisation domain containing 20 ( MGI:1914239)
Sequence:	sense strand is shown >T32086 CCTCTGCCCAGTTGTTGGAGCTCTTCTTTGTGTGGGTACCCCTAACCAGGCCTCCCTGCCCTCCTGTGGT TTCTCACCGTTAGGAGATGCCTCTTCCTGTCTCCTTCCCTTCTGAGTTGTTACTGTGCCCTTTTCCGGGT CTGCCTCCGGCTGGGTGAAGAAGCTCTCTGGTAATTAGGCGGCCAGCTTTACCAGCCCTGGCAAACGAGC TTTTGGGTCTGGTGCTCTTGGTTGAATCTTTGCATTCACCTCCTGGTGCAGGAGAGGAAGTGAACTGTTG CCTCACATTCGGCACAGCAGCCCACTCTTACACCGGGTGAGTGGTTTCACAATCAGACAAATTGCTTATT AGCAAAAGGACCAAAACGTCAGAGAAACAGTCTGTGCTGGGGTTGGGTGTGCCCACCTGGGGAGGTGCGG GGCAGGCGAAGGGAATTCCCTGGAGCTCCAGCTCAGCTCTGCTTTTTACTAACATAGGAGCAAACCAACC CCCTAAGAAGGGCAGCCTGATGCTTCATTTGGAAGTGAAACTTGCAAATGAGGTGGCAGAATGGCTCCAG ATTGGGCCAGCTGGACCCTCCTCTGGTGGGAAGCCACAGCGCCTGTCCATTAAATAGGCCATTAGCTACT GGAACATGGTAAGAAAGTAATATGTGGTCATCCCCCAAATCAGGTCCAGGCCTTGTTGTTGTGTTATCTG CTCAGGCTTACACGTGTGGTAGACGGCAGCCCAGATCATTCCCTAGCTCCTTCCTCTCACATTTCCATGG CTGTTTGCAGAGCCAAGAGCCTGACAGCAATGTCTGCAATTATTTTACTTCCCGGTAGAAGTCCACCTTT GAGTCCGTCCTCCACCCTGGGCGGTGTGTAAGCTGTTTCCTTCAAGCTCTTTACAGAATATGTTGTTTCT TGAAGGCTGCTCCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5042309), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58038. Forward Primer - name:058038_F_IRAV90_d10_2410004N11Rik, sequence:CCTCTGCCCAGTTGTTGG; Reverse Primer - name:058038_R_SP6_IRAV90_d10_2410004N11Rik, sequence:TGGGAGCAGCCTTCAAGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8890 same embryo
	EMAGE:8889 same embryo
	EMAGE:8888 same embryo
	EMAGE:8886 same embryo
	EMAGE:8885 same embryo
	EurExpress:euxassay_010084 same experiment
	MGI:4825738 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source