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EMAGE:8923

Gpd1l glycerol-3-phosphate dehydrogenase 1-like ( MGI:1289257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8923 EMAGE:8923 EMAGE:8923 EMAGE:8923 EMAGE:8923
"Pseudo-wholemount" of euxassay_012850. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012850_01 euxassay_012850_02 euxassay_012850_03 euxassay_012850_04
EMAGE:8923 EMAGE:8923 EMAGE:8923 EMAGE:8923 EMAGE:8923
euxassay_012850_05 euxassay_012850_06 euxassay_012850_07 euxassay_012850_08 euxassay_012850_09
EMAGE:8923 EMAGE:8923 EMAGE:8923 EMAGE:8923 EMAGE:8923
euxassay_012850_10 euxassay_012850_11 euxassay_012850_12 euxassay_012850_13 euxassay_012850_14
EMAGE:8923 EMAGE:8923 EMAGE:8923 EMAGE:8923 EMAGE:8923
euxassay_012850_15 euxassay_012850_16 euxassay_012850_17 euxassay_012850_18 euxassay_012850_19
EMAGE:8923 EMAGE:8923 EMAGE:8923 EMAGE:8923 EMAGE:8923
euxassay_012850_20 euxassay_012850_21 euxassay_012850_22 euxassay_012850_23 euxassay_012850_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8923Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8923_wholemount_strong.wlz
8923_wholemount_moderate.wlz
8923_wholemount_weak.wlz
8923_wholemount_possible.wlz
8923_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8923_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 08 09 14 15
pons mantle layer
weak weak
regionalweak expression: see section 07 17
facial vii ganglion
weak weak
regionalweak expression: see section 04 06 21
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 06 16 17
trigeminal v ganglion
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22
ventral grey horn
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14
dorsal root ganglion
weak weak
regionalweak expression: see section 05 06 07 08 09 10 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38501
Entity Detected:Gpd1l, glycerol-3-phosphate dehydrogenase 1-like ( MGI:1289257)
Sequence:sense strand is shown

>T38501
AAGATGTGGGTCTTTGAGGAGACCGTGAACGGGAGGAAGCTGACAGACATAATCAACAATGACCACGAAA
ACGTGAAATATCTCCCAGGACACAAGCTGCCAGAGAATGTGGTCGCTGTCCCAAACCTCAGCGAAGCCGT
GCAGGACGCGGACCTGCTGGTGTTCGTCATCCCTCACCAGTTCATCCACAAGATCTGCGATGAGATCACG
GGCAGGGTGCCCGAGAAGGCCCTGGGGATCACCCTCATCAAGGGCATAGATGAGGGCCCCGACGGGCTGA
AGCTAATCTCCGACATCATCCGAGAGAAGATGGGGATCGACATCAGTGTGCTGATGGGGGCCAACATCGC
CAGTGAGGTCGCTGCGGAGAAGTTCTGCGAGACCACCATTGGCAGCAAAGTGATGCAGAACGGCCTTCTC
TCAAAAGAGCTGCTGCAGACGCCCAACTTCAGAATCACTGTGGTAGACGATGCAGACACCGTGGAGCTTT
GGGGTGCTCTTAAGAACATTGTTGCTGTGGTAGCTGGCTTCTGCGACGGCCTCCGCTGTGGGGACAACAC
CAAGGCGGCCGTCATACGCCTGGGCCTCATGGAAATGATCGCTTTCGCCAAGATCTTCTGTAAGGGCCAG
GTGTCCACGGCCACCTTCTTGGAGAGCTGCGGGGTGGCTGACCTCATCACCACCTGCTACGGAGGGCGGA
ACCGCAGGGTGGCAGAGGCCTTCGCCAGGACTGGGAAGACCATCGAAGAGCTGGAGAAGGAGCTGCTGAA
C
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 148826. Forward Primer - name:148826_F_cDNA_Gpd1l, sequence:AAGATGTGGGTCTTTGAGGAGA; Reverse Primer - name:148826_N_SP6_cDNA_Gpd1l, sequence:GTTCAGCAGCTCCTTCTCCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8920 same embryo
 EMAGE:8924 same embryo
 EMAGE:8919 same embryo
 EMAGE:8922 same embryo
 EMAGE:8921 same embryo
 EurExpress:euxassay_012850 same experiment
 MGI:4825160 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS