EMAGE mouse gene expression spatial database

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EMAGE:8923

Gpd1l glycerol-3-phosphate dehydrogenase 1-like ( MGI:1289257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012850. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012850_01	euxassay_012850_02	euxassay_012850_03	euxassay_012850_04

euxassay_012850_05	euxassay_012850_06	euxassay_012850_07	euxassay_012850_08	euxassay_012850_09

euxassay_012850_10	euxassay_012850_11	euxassay_012850_12	euxassay_012850_13	euxassay_012850_14

euxassay_012850_15	euxassay_012850_16	euxassay_012850_17	euxassay_012850_18	euxassay_012850_19

euxassay_012850_20	euxassay_012850_21	euxassay_012850_22	euxassay_012850_23	euxassay_012850_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8923_wholemount_strong.wlz

8923_wholemount_moderate.wlz

8923_wholemount_weak.wlz

8923_wholemount_possible.wlz

8923_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8923_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 14 15

pons mantle layer

weak

regional

weak expression: see section 07 17

facial vii ganglion

weak

regional

weak expression: see section 04 06 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 16 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

ventral grey horn

weak

regional

weak expression: see section 08 09 10 11 12 13 14

dorsal root ganglion

weak

regional

weak expression: see section 05 06 07 08 09 10 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38501
Entity Detected:	Gpd1l, glycerol-3-phosphate dehydrogenase 1-like ( MGI:1289257)
Sequence:	sense strand is shown >T38501 AAGATGTGGGTCTTTGAGGAGACCGTGAACGGGAGGAAGCTGACAGACATAATCAACAATGACCACGAAA ACGTGAAATATCTCCCAGGACACAAGCTGCCAGAGAATGTGGTCGCTGTCCCAAACCTCAGCGAAGCCGT GCAGGACGCGGACCTGCTGGTGTTCGTCATCCCTCACCAGTTCATCCACAAGATCTGCGATGAGATCACG GGCAGGGTGCCCGAGAAGGCCCTGGGGATCACCCTCATCAAGGGCATAGATGAGGGCCCCGACGGGCTGA AGCTAATCTCCGACATCATCCGAGAGAAGATGGGGATCGACATCAGTGTGCTGATGGGGGCCAACATCGC CAGTGAGGTCGCTGCGGAGAAGTTCTGCGAGACCACCATTGGCAGCAAAGTGATGCAGAACGGCCTTCTC TCAAAAGAGCTGCTGCAGACGCCCAACTTCAGAATCACTGTGGTAGACGATGCAGACACCGTGGAGCTTT GGGGTGCTCTTAAGAACATTGTTGCTGTGGTAGCTGGCTTCTGCGACGGCCTCCGCTGTGGGGACAACAC CAAGGCGGCCGTCATACGCCTGGGCCTCATGGAAATGATCGCTTTCGCCAAGATCTTCTGTAAGGGCCAG GTGTCCACGGCCACCTTCTTGGAGAGCTGCGGGGTGGCTGACCTCATCACCACCTGCTACGGAGGGCGGA ACCGCAGGGTGGCAGAGGCCTTCGCCAGGACTGGGAAGACCATCGAAGAGCTGGAGAAGGAGCTGCTGAA C
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 148826. Forward Primer - name:148826_F_cDNA_Gpd1l, sequence:AAGATGTGGGTCTTTGAGGAGA; Reverse Primer - name:148826_N_SP6_cDNA_Gpd1l, sequence:GTTCAGCAGCTCCTTCTCCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8920 same embryo
	EMAGE:8924 same embryo
	EMAGE:8919 same embryo
	EMAGE:8922 same embryo
	EMAGE:8921 same embryo
	EurExpress:euxassay_012850 same experiment
	MGI:4825160 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source