EMAGE mouse gene expression spatial database

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EMAGE:8929

Hist1h1t histone cluster 1, H1t ( MGI:1888530)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012858. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012858_01	euxassay_012858_02	euxassay_012858_03	euxassay_012858_04

euxassay_012858_05	euxassay_012858_06	euxassay_012858_07	euxassay_012858_08	euxassay_012858_09

euxassay_012858_10	euxassay_012858_11	euxassay_012858_12	euxassay_012858_13	euxassay_012858_14

euxassay_012858_15	euxassay_012858_16	euxassay_012858_17	euxassay_012858_18	euxassay_012858_19

euxassay_012858_20	euxassay_012858_21	euxassay_012858_22	euxassay_012858_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8929_wholemount_strong.wlz

8929_wholemount_moderate.wlz

8929_wholemount_weak.wlz

8929_wholemount_possible.wlz

8929_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8929_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 09 10 11 12

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 14 16 weak expression: see section 15

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 moderate expression: see section 12 13 14

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 moderate expression: see section 12 13 14

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 11 12 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 18 19 weak expression: see section 03 04 05 07 09 16 17

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 18 19 20 weak expression: see section 03 04 08 09

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 15 16 17 moderate expression: see section 13 14

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

renal cortex

weak

regional

weak expression: see section 05 06 07 08 15 16 17 18

orbito-sphenoid

moderate

regional

moderate expression: see section 03 04 05 19 20 21 22 weak expression: see section 06 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38514
Entity Detected:	Hist1h1t, histone cluster 1, H1t ( MGI:1888530)
Sequence:	sense strand is shown >T38514 GTGAATAAAGGAGTCCTGGTGCAGACCAAGGGCACTGGCGCCTCAGGCTCCTTCAAGCTCAGTAAGAAGG CGGCTTCTGGGAACGACAAGGGCAAGGGCAAGAAATCTGCTTCTGCCAAGGCTAAGAAGATGGGCTTGCC CCGGGCCTCCAGATCTCCCAAGAGTAGCAAGACCAAGGCTGTCAAGAAGCCAAAGGCTACGCCCACAAAA GCTTCTGGGAGCAGAAGGAAGACCAAAGGGGCCAAGGGCGTGCAGCAACGTAAAAGCCCCGCCAAAGCCA GGGCAGCAAACCCCAATTCTGGGAAGGCAAAGATGGTCATGCAGAAGACCGATCTGAGGAAGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 150696. Forward Primer - name:150696_F_cDNA_Hist1h1t, sequence:GTGAATAAAGGAGTCCTGGTGC; Reverse Primer - name:150696_N_SP6_cDNA_Hist1h1t, sequence:GCCTTCCTCAGATCGGTCTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8928 same embryo
	EMAGE:8933 same embryo
	EMAGE:8931 same embryo
	EMAGE:8930 same embryo
	EMAGE:8932 same embryo
	EurExpress:euxassay_012858 same experiment
	MGI:4825377 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source