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EMAGE:9190

Gnas GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus ( MGI:95777)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9190 EMAGE:9190 EMAGE:9190 EMAGE:9190 EMAGE:9190
euxassay_017957_01 euxassay_017957_02 euxassay_017957_03 euxassay_017957_04 euxassay_017957_05
EMAGE:9190 EMAGE:9190 EMAGE:9190 EMAGE:9190 EMAGE:9190
euxassay_017957_06 euxassay_017957_07 euxassay_017957_08 euxassay_017957_09 euxassay_017957_10
EMAGE:9190 EMAGE:9190 EMAGE:9190 EMAGE:9190 EMAGE:9190
euxassay_017957_11 euxassay_017957_12 euxassay_017957_13 euxassay_017957_14 euxassay_017957_15
EMAGE:9190 EMAGE:9190 EMAGE:9190 EMAGE:9190 EMAGE:9190
euxassay_017957_16 euxassay_017957_17 euxassay_017957_18 euxassay_017957_19 euxassay_017957_20
EMAGE:9190 EMAGE:9190 EMAGE:9190 EMAGE:9190
euxassay_017957_21 euxassay_017957_22 euxassay_017957_23 euxassay_017957_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9190Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9190_wholemount_strong.wlz
9190_wholemount_moderate.wlz
9190_wholemount_weak.wlz
9190_wholemount_possible.wlz
9190_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9190_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50484
Entity Detected:Gnas, GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus ( MGI:95777)
Sequence:sense strand is shown

>T50484
AGAGTCTGGCAAAAGCACCATTGTGAAGCAGATGAGGATCCTGCATGTTAATGGGTTTAACGGAGAGGGC
GGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGTGAGAAGGCCACTAAAGTGCAGGACATCA
AAAACAACCTGAAGGAGGCCATTGAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCTGTGGAGCT
GGCCAACCCTGAGAACCAGTTCAGAGTGGACTACATTCTGAGCGTGATGAACGTGCCCGACTTTGACTTC
CCACCTGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAGGGAGTGCGTGCCTGCTACGAGCGCT
CCAATGAGTACCAGCTGATTGACTGTGCCCAGTACTTCCTGGACAAGATTGATGTGATCAAGCAGGCCGA
CTACGTGCCAAGTGACCAGGACCTGCTTCGCTGCCGTGTCCTGACCTCTGGAATCTTTGAGACCAAGTTC
CAGGTGGACAAAGTCAACTTCCACATGTTCGATGTGGGCGGCCAGCGCGATGAACGCCGCAAGTGGATCC
AGTGCTTCAATGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTACAACATGGTCATTCGGGA
GGACAACCAGACTAACCGCCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTGGAACAACAGATGGCTG
CGCACCATCTCTGTGATTCTCTTCCTCAACAAGCAAGACCTGCTTGCTGAGAAAGTCCTCGCTGGCAAAT
CGAAGATTGAGGACTACTTTCCAGAGTTCGCTCGCTACACCA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGAGTCTGGCAAAAGCAC; Reverse Primer - name:unspecified, sequence:TGGTGTAGCGAGCGAACT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9189 same embryo
 EMAGE:9191 same embryo
 EMAGE:9188 same embryo
 EMAGE:9187 same embryo
 EurExpress:euxassay_017957 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS