EMAGE mouse gene expression spatial database

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EMAGE:9241

Jun Jun oncogene ( MGI:96646)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017995. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017995_01	euxassay_017995_02	euxassay_017995_03	euxassay_017995_04

euxassay_017995_05	euxassay_017995_06	euxassay_017995_07	euxassay_017995_08	euxassay_017995_09

euxassay_017995_10	euxassay_017995_11	euxassay_017995_12	euxassay_017995_13	euxassay_017995_14

euxassay_017995_15	euxassay_017995_16	euxassay_017995_17	euxassay_017995_18	euxassay_017995_19

euxassay_017995_20	euxassay_017995_21	euxassay_017995_22	euxassay_017995_23	euxassay_017995_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9241_wholemount_strong.wlz

9241_wholemount_moderate.wlz

9241_wholemount_weak.wlz

9241_wholemount_possible.wlz

9241_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9241_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50571
Entity Detected:	Jun, Jun oncogene ( MGI:96646)
Sequence:	sense strand is shown >T50571 TAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGCAAACGTTTTGAGAACAGACTGTCAGGGCTGAG GGGCAATGGAAGAAAAAAAATAACAGAGACAAACTTGAGAACTTGACTGGTTGCGACAGAGAAAAAAAAA GTGTCCGAGTACTGAAGCCAAGGGTACACAAGATGGACTGGGTTGCGACCTGACGGCGCCCCCAGTGTGC TGGAGTGGGAAGGACGTGGCGCGCCTGGCTTTGGCGTGGAGCCAGAGAGCAGCGGCCTATTGGCCGGCAG ACTTTGCGGACGGGCTGTGCCCGCGCGCGACCAGAACGATGGACTTTTCGTTAACATTGACCAAGAACTG CATGGACCTAACATTCGATCTCATTCAGTATTAAAGGGGGGTGGGAGGGGTTACAAACTGCAATAGAGAC TGTAGATTGCTTCTGTAGTGCTCCTTAACACAAAGCAGGGAGGGCTGGGAAGGGGGGGAGGCTTGTAAGT GCCAGGCTAGACTGCAGATGAACTCCCCTGGCCTGCCTCTCTCAACTGTGTATGTACATATATATTTTTT TTTAATTTGATGAAAGCTGATTACTGTCAATAAACAGCTTCCTGCCTTTGTAAGTTATTCCATGTTTGTT TGTTTGGGTGTCCTGCCCAGTGTTTGTAAATAAGAGATTTGAAGCATTCTGAGTTTACCATTTGTAATAA AGTATATAATTTTTTTATGTTTTGTTTCTGAAAATTTCCAGAAAGGATATTTAAGAAAATACAATAAACT ATTGAAAAGTAGCCCCCAACCTCTTTGCTGCATTATCCATAGATAATGATAGCTAGATGAAGTGACAGCT GAGTGCCCCCAATATACTAGGGTGAAAGCTGTGTCC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TAACAGTGGGTGCCAACTCA; Reverse Primer - name:unspecified, sequence:GGACACAGCTTTCACCCTA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9243 same embryo
	EMAGE:9240 same embryo
	EMAGE:9244 same embryo
	EMAGE:9242 same embryo
	EurExpress:euxassay_017995 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source