EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9331

Dlx3 distal-less homeobox 3 ( MGI:94903)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018030. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018030_01	euxassay_018030_02	euxassay_018030_03	euxassay_018030_04

euxassay_018030_05	euxassay_018030_06	euxassay_018030_07	euxassay_018030_08	euxassay_018030_09

euxassay_018030_10	euxassay_018030_11	euxassay_018030_12	euxassay_018030_13	euxassay_018030_14

euxassay_018030_15	euxassay_018030_16	euxassay_018030_17	euxassay_018030_18	euxassay_018030_19

euxassay_018030_20	euxassay_018030_21	euxassay_018030_22	euxassay_018030_23	euxassay_018030_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9331_wholemount_strong.wlz

9331_wholemount_moderate.wlz

9331_wholemount_weak.wlz

9331_wholemount_possible.wlz

9331_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9331_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

moderate

spotted

moderate expression: see section 09 13 weak expression: see section 10 11

rib

strong

regional

strong expression: see section 01 02 03 05 17 18 19 20 21 moderate expression: see section 04 06 22 weak expression: see section 16 23

vibrissa

strong

regional

strong expression: see section 03 04 05 19 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 weak expression: see section 07 08 13 14 15 16 17

vomeronasal organ

moderate

regional

moderate expression: see section 11 weak expression: see section 14

mandible

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 14 17 18 19 20 moderate expression: see section 15 16 21 22

lower jaw incisor

strong

regional

strong expression: see section 09 10 13 14

lower jaw molar

strong

regional

strong expression: see section 05

maxilla

strong

regional

strong expression: see section 05 06 07 08 09 17 18 19 20 moderate expression: see section 04 16 21

upper jaw incisor

strong

regional

strong expression: see section 09 10 13 14 moderate expression: see section 15

upper jaw molar

strong

regional

strong expression: see section 05 19

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 20 21 moderate expression: see section 22 23 24

clavicle

strong

regional

strong expression: see section 06 07 14 15 weak expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50311
Entity Detected:	Dlx3, distal-less homeobox 3 ( MGI:94903)
Sequence:	sense strand is shown >T50311 CAGCACTCTGGGACACATCTTCCCATTCCACGCCAGCCCCTGCCCGCAATCCGCTGCCCCCACCGCTCCC ATACAGTGCCTCCCCCAACTACCTGGACGACCCCACCAACTCCTGGTACCACACACAGAACCTCAGTGGA CCCCACTTACAGCAGCAGCCTCCTCAGCCGGCTACCCTGCACCATGCCTCCCCTGGGCCCCCGCCTAACC CTGGGGCTGTGTACTGAGTACCCACCTGGCCTGCGCCCCTCCACGAAGGACCCCCTCCAGGACCAGGCAG AAGGTGCCCTGTCCTAGCGACACTCAGGAATCATTGAGGGGCACAGGGGGAAAGACTCCCTTCCCTCTCC CTTGTCCCTTCTTCCAGGGGCCCAACAACCTCCAGATGACAAATGCATGGACCGAGGATGCCCCCCAATC TCCCTCCCCTTGCTTAGACTGGGGTGCCCTCCAGACGGCGAGGAGTTCTACCCCAGTGGGGACAGCACAT GCTCTCTGCTCCAGGAACCCGGATTGCCTCTAGATGGCTCATCACTTTCCAGCTTTTCAAACACAGTAGA GACCTCCAAAATGGGAGCCAGAGTGTTTGCAGGTCCACCTGT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAGCACTCTGGGACACATCTTC; Reverse Primer - name:unspecified, sequence:ACAGGTGGACCTGCAAACACTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9329 same embryo
	EMAGE:9328 same embryo
	EMAGE:9332 same embryo
	EMAGE:9330 same embryo
	EurExpress:euxassay_018030 same experiment
	MGI:4824328 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source