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EMAGE:9495

Bhlhe22 basic helix-loop-helix family, member e22 ( MGI:1930001)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9495 EMAGE:9495 EMAGE:9495 EMAGE:9495 EMAGE:9495
"Pseudo-wholemount" of euxassay_018082. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018082_01 euxassay_018082_02 euxassay_018082_03 euxassay_018082_04
EMAGE:9495 EMAGE:9495 EMAGE:9495 EMAGE:9495 EMAGE:9495
euxassay_018082_05 euxassay_018082_06 euxassay_018082_07 euxassay_018082_08 euxassay_018082_09
EMAGE:9495 EMAGE:9495 EMAGE:9495 EMAGE:9495 EMAGE:9495
euxassay_018082_10 euxassay_018082_11 euxassay_018082_12 euxassay_018082_13 euxassay_018082_14
EMAGE:9495 EMAGE:9495 EMAGE:9495 EMAGE:9495 EMAGE:9495
euxassay_018082_15 euxassay_018082_16 euxassay_018082_17 euxassay_018082_18 euxassay_018082_19
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euxassay_018082_20 euxassay_018082_21 euxassay_018082_22 euxassay_018082_23 euxassay_018082_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9495Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9495_wholemount_strong.wlz
9495_wholemount_moderate.wlz
9495_wholemount_weak.wlz
9495_wholemount_possible.wlz
9495_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9495_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50148
Entity Detected:Bhlhe22, basic helix-loop-helix family, member e22 ( MGI:1930001)
Sequence:sense strand is shown

>T50148
CTCTTCCCAGCTCCGCGGCCGCGGCTGCGGCGGCCGCTGCCCTGCACCCGGCGCTCGGCGCCTACGAGCA
GGCGGCCGGCTACCCGTTCAGTGCAGGGCTGCCGCCCGCTGCCTCCTGCCCCGAGAAGTGTGCCCTATTC
AACAGCGTCTCGTCCAGCCTCTGCAAACAGTGCACGGAGAAGCCTTAAACACTCCCTTCTTCGAAAACCT
CGAGACCGACCCCAAAGCTAGAGGAAAGTGAGAAGCTGCTCCCCATTCCTTTTCCTTTGGTCCTCTCCTA
AGTTGTGAAACACTTGCAGGGCAAACAAAGCAGAGGCAGGAACTTACAAGTATCAGAGACAAACCGGACA
TTCAGCCTTGGCAAGCCCGGAATCTCGGTCTTTGGGGTGGGGAGGGAGGGGAGAGGGAGGTTTAGTGCGG
ATGAAGTGTGCTTCCTTTTTCCCCTCAGAAAAGTGGCAACTTTGGTGGCTCCCTACATCTCAAGGAAGTA
CAGTCTTCTTTGGAGGCGAAAATAAGTTGAAAAGTAGTGCTTGATTATTAGACGAGAAAAGTACTGAGTG
GCAAAATACTAATCCTACTAACACCTGACAATTGGCAAGTGATGAAAGGGAGAGGGGTTCAGAGTTGAGA
GGCTCGGGGACAGAATATTCATTCAGACAAATCAGAAAGGGCACTCGAAAATACATCTTGATTCTGAGCC
AGGAGAAAAACTTGAAATGCAGACTTTATTTAAGTGGAGGGGAAAGCAGAGAGACATGTTGCTATGAAAG
ACTTGTATTTTTTATTGTCTCTGAACTTAAAATTCCGTGAAAATGCTCAAAGTTTTGGTGAGAGTGATAT
TAAAAAGCAGGCTGTGGCTGAAAGAATTGCAT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AAACAAAGCAGAGGCAGGAA; Reverse Primer - name:unspecified, sequence:ACATGGACGATGCAACTGAA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9497 same embryo
 EMAGE:9496 same embryo
 EMAGE:9494 same embryo
 EMAGE:9493 same embryo
 EMAGE:9492 same embryo
 EurExpress:euxassay_018082 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS