EMAGE mouse gene expression spatial database

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EMAGE:9877

Ikbip IKBKB interacting protein ( MGI:1914704)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008119. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008119_01	euxassay_008119_02	euxassay_008119_03	euxassay_008119_04

euxassay_008119_05	euxassay_008119_06	euxassay_008119_07	euxassay_008119_08	euxassay_008119_09

euxassay_008119_10	euxassay_008119_11	euxassay_008119_12	euxassay_008119_13	euxassay_008119_14

euxassay_008119_15	euxassay_008119_16	euxassay_008119_17	euxassay_008119_18	euxassay_008119_19

euxassay_008119_20	euxassay_008119_21	euxassay_008119_22	euxassay_008119_23	euxassay_008119_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9877_wholemount_strong.wlz

9877_wholemount_moderate.wlz

9877_wholemount_weak.wlz

9877_wholemount_possible.wlz

9877_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9877_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2
Entity Detected:	Ikbip, IKBKB interacting protein ( MGI:1914704)
Sequence:	sense strand is shown >T2 TCGACTCCTATAGGGCGATTGACGCGTNCNTTACCCTGAAGATTAACTCCGCGGATCAAGAAATAAAATC GCTCACTGAACGTCTAAAAGATTTAGAAGACAGCACACTGCGAAACATCAGAACAGTGAGCAGGCAAGAA GAGGAAGATCTTCTACGAGTCGAGGCCCAGCTTAGCTCTGATACCAAAGCAGTTAAGAAGCTAGAAGAAG AACAGCACACGCTCCTAGCCAGAGACGAAGATCTGACCAACAAACTCTCCAGCTACGAACCCAAAGTAGA AGAGTGCAAGGCACACTTGCCAACCATAGAAAATGCTGTCCACTCGGTTCTCAGAGTCTCTCAGGATCTG ATAGGGACAGAAAGGAAGATGGAGGAGCTGACGATGCAGATGTTCAACATGGAGGACGACATGCTGAGGG CTGTGTCTGAGATCATGGAGATGCAGAAAACCCTGGAGGGGATCCAGTATGACAACAGCTTACTAAAGAT GCAGAACGAGCTGGTCGTTCTAAAAGGAAAAGTTCATGACTTCATAGCATATTCAAGTGCACGGGAAAAG G
Notes:	The probe template was PCR amplified from IMAGE:778055 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:778055 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9879 same embryo
	EMAGE:9878 same embryo
	EMAGE:9876 same embryo
	EurExpress:euxassay_008119 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source