EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9897

Mllt3 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 ( MGI:1917372)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008130. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008130_01	euxassay_008130_02	euxassay_008130_03	euxassay_008130_04

euxassay_008130_05	euxassay_008130_06	euxassay_008130_07	euxassay_008130_08	euxassay_008130_09

euxassay_008130_10	euxassay_008130_11	euxassay_008130_12	euxassay_008130_13	euxassay_008130_14

euxassay_008130_15	euxassay_008130_16	euxassay_008130_17	euxassay_008130_18	euxassay_008130_19

euxassay_008130_20	euxassay_008130_21	euxassay_008130_22	euxassay_008130_23	euxassay_008130_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9897_wholemount_strong.wlz

9897_wholemount_moderate.wlz

9897_wholemount_weak.wlz

9897_wholemount_possible.wlz

9897_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9897_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22

hindlimb digit 1 metatarsal

moderate

regional

moderate expression: see section 03 04 05 20 21 22 23

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 02 03 04 05 19 20 21 22 23

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 02 03 04 05 19 20 21 22 23

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 03 04 05 19 20 21 22

hindlimb digit 5 metatarsal

moderate

regional

moderate expression: see section 03 04 05 20 21 22

adrenal gland

moderate

regional

moderate expression: see section 09 10 17 18 19 weak expression: see section 08

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 19 20 weak expression: see section 17 18

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 05 06 07 08 moderate expression: see section 09 10 21 22 23 24

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

ear

moderate

regional

moderate expression: see section 01 02 03 04 05 19 20 21 23 weak expression: see section 22 24

inner ear

moderate

regional

moderate expression: see section 06 07 08 09 16 17 18

cornea epithelium

weak

regional

weak expression: see section 01 02 03 04 05 24

hindgut

moderate

regional

moderate expression: see section 13 weak expression: see section 15 16

rectum

moderate

regional

moderate expression: see section 14 weak expression: see section 13

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17 weak expression: see section 14

lower jaw molar

weak

regional

weak expression: see section 06 07 20

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 17 weak expression: see section 14

upper jaw molar

weak

regional

weak expression: see section 06 07 20 21

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 17 18 19 20 21 22 23 24 weak expression: see section 12 16

genital tubercle of male

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T18
Entity Detected:	Mllt3, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 ( MGI:1917372)
Sequence:	sense strand is shown >T18 TCNTCTATTTATTACCACATACAGATAAAGTTGTGAGGGAATGACACCACCAGACGTCCTAAATACTCGG TCCCGCGGATGCTCTTTCAATGCAGCGCCCTTGCCGTCCCAAGCCCAGTGACCTTATTCGTATACTGTGC CACTTTCCACCAACTCTTCCCAGGTCCTTTAACTTGTTGCAGTCTATGGTTTCCATCTTTGTTTTCCCAG TTCCAGGACATAGTTACCAACTGGGGGGACCAAATAGCCACCTTGATTTTCTTCCTTGTGGTCTTCTTTC CTAAAGGTTGAGGCCCAGTCCTTGGCTGTATCCATGTAATGATCTTGGACATGGTAGATGTACCAAATAG GATCATTTGAATTGCTGTCTAGCCTTAGTCAATAAACCTGTAGGACTTTGAACAAAAGTGTCCCTGTGAT GTCCACAGCCCAGCAGTGCCGAGCTGTCACCAACACTCGTGTCTCTATTTTACTGTTGCAATATTAGGTG AATATGGAGGTAAAGGCATTCCACAGACTCAGCATCTTAAAT
Notes:	The probe template was PCR amplified from IMAGE:833180 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:833180 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9898 same embryo
	EMAGE:9900 same embryo
	EMAGE:9899 same embryo
	EurExpress:euxassay_008130 same experiment
	MGI:4826393 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source