EMAGE mouse gene expression spatial database

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EMAGE:9906

Wdtc1 WD and tetratricopeptide repeats 1 ( MGI:2685541)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008145. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008145_01	euxassay_008145_02	euxassay_008145_03	euxassay_008145_04

euxassay_008145_05	euxassay_008145_06	euxassay_008145_07	euxassay_008145_08	euxassay_008145_09

euxassay_008145_10	euxassay_008145_11	euxassay_008145_12	euxassay_008145_13	euxassay_008145_14

euxassay_008145_15	euxassay_008145_16	euxassay_008145_17	euxassay_008145_18	euxassay_008145_19

euxassay_008145_20	euxassay_008145_21	euxassay_008145_22	euxassay_008145_23	euxassay_008145_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9906_wholemount_strong.wlz

9906_wholemount_moderate.wlz

9906_wholemount_weak.wlz

9906_wholemount_possible.wlz

9906_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9906_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

heart left ventricle

moderate

spotted

moderate expression: see section 09 10 weak expression: see section 11 12

heart right ventricle

moderate

spotted

moderate expression: see section 14 17 18 weak expression: see section 13

left lung

strong

spotted

strong expression: see section 02 03 moderate expression: see section 04 05 06 07 08 09 10 11

right lung

moderate

spotted

moderate expression: see section 17 18 19 20 21 22 23 weak expression: see section 13 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55
Entity Detected:	Wdtc1, WD and tetratricopeptide repeats 1 ( MGI:2685541)
Sequence:	sense strand is shown >T55 ANGTGATGTCCCGGCCTAATGCGTCACATGCACTGCTGTGGGCCTGCTCTGGGAACTTCCCTTTGAAGTC ATCCAGGCACTCCAGAGCCTCGGCCACATACTTCAGTTCAAAGAGGCACCGGGCCAGACGGAAGTGTGCC TTCAGGTGGCAAGGGTTTAGGGAGATGGCCTTGAGGCAGTCCCGGAGGGCATCATAGTGGTCCCCGTCCC ACTTGCGCTTCATGTAGGCGGCAGCTCGGTTGCCATAGAGCATAGCGTTGTGCGGGGCCTTCTGCACAGC CTGGCTGTAGAGCTGGATGGCCTGGGTCCACTGCTGGCAGGCGAAGGCTTCATTGGCCTGCTGCTTCACA CGCTCCAGGTATGGGGGCAGCTCCACCTGGGGGCTGATGCACCCCTTGCTCTCTGGCAGCCGGAAGCCGT TGCTGTGAAGGTGTAGGCCATTCGACACGCCGTTGGACACCCCGTTAGTAGACATCTTGCCGTTCTGGAC CACGGAGTGGCA
Notes:	The probe template was PCR amplified from IMAGE:1108011 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1108011 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9905 same embryo
	EMAGE:9903 same embryo
	EMAGE:9904 same embryo
	EurExpress:euxassay_008145 same experiment
	MGI:4829212 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source