EMAGE mouse gene expression spatial database

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EMAGE:10369

Tsc22d3 TSC22 domain family, member 3 ( MGI:1196284)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000517. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000517_01	euxassay_000517_02	euxassay_000517_03	euxassay_000517_04

euxassay_000517_05	euxassay_000517_06	euxassay_000517_07	euxassay_000517_08	euxassay_000517_09

euxassay_000517_10	euxassay_000517_11	euxassay_000517_12	euxassay_000517_13	euxassay_000517_14

euxassay_000517_15	euxassay_000517_16	euxassay_000517_17	euxassay_000517_18	euxassay_000517_19

euxassay_000517_20	euxassay_000517_21	euxassay_000517_22	euxassay_000517_23	euxassay_000517_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10369_wholemount_strong.wlz

10369_wholemount_moderate.wlz

10369_wholemount_weak.wlz

10369_wholemount_possible.wlz

10369_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10369_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

otic capsule

weak

regional

weak expression: see section 22 23 24

inner ear vestibular component

weak

regional

weak expression: see section 22 23

facial bone primordium

moderate

regional

moderate expression: see section 07

optic foramen

moderate

regional

moderate expression: see section 07

orbital fissure

weak

regional

weak expression: see section 19 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 07 08 weak expression: see section 19 20 22 23

viscerocranium

moderate

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3632
Entity Detected:	Tsc22d3, TSC22 domain family, member 3 ( MGI:1196284)
Sequence:	sense strand is shown >T3632 GCGCATGCATTTTTTTTCTATATAGCCATAATGCGTATATATACCCATAGGGCTAGATCTATATCTTAGG GTAGTGATGTATACATATACACATACACCTACATGTTGAAGGGCCTAACCAGCTTTGGGAGTACTGACTG GTCTCTTATCTCTTAAAGCTAAGTTTTTGACTGTGCTAATTTACCAAATTGATCCAGTTTGTCCTTTAGA TTAAATAAGACTCGATATGAGGGAGGGAGGGGAGACCAGCCTCACAATGCGGCCACAGATGCCTTGCTGC TGCAGTCCTCCCTGATCTGTCCACTGAAGACATGAAGTCCTCTTTTGAATGCCAAACCCACCATTCATTG GTGCTGACTACATAGAATGGGGTTGAGAGAAGATCAGTTTGGACTTCACATTTTTGTTTTAAGTTTTAGG TTGTTTTTTTTTGGTTTTGTTTGTTTGTTTGTTTGTTTTTGTTTTTTGTTTTTCTTTTTTTTAAGTTCTT GTGGGAAACTTTGGGGTTAATCAAAGGATGTAGTCCTGTGGTAGACCAGAGGAGTGACTAGTTTTGA
Notes:	The probe template was PCR amplified from IMAGE:3168065 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3168065 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10367 same embryo
	EMAGE:10372 same embryo
	EMAGE:10368 same embryo
	EMAGE:10371 same embryo
	EMAGE:10370 same embryo
	EurExpress:euxassay_000517 same experiment
	MGI:4828948 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source