EMAGE mouse gene expression spatial database

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EMAGE:10523

Dolpp1 dolichyl pyrophosphate phosphatase 1 ( MGI:1914093)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000817. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000817_01	euxassay_000817_02	euxassay_000817_03	euxassay_000817_04

euxassay_000817_05	euxassay_000817_06	euxassay_000817_07	euxassay_000817_08	euxassay_000817_09

euxassay_000817_10	euxassay_000817_11	euxassay_000817_12	euxassay_000817_13	euxassay_000817_14

euxassay_000817_15	euxassay_000817_16	euxassay_000817_17	euxassay_000817_18	euxassay_000817_19

euxassay_000817_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10523_wholemount_strong.wlz

10523_wholemount_moderate.wlz

10523_wholemount_weak.wlz

10523_wholemount_possible.wlz

10523_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10523_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3894
Entity Detected:	Dolpp1, dolichyl pyrophosphate phosphatase 1 ( MGI:1914093)
Sequence:	sense strand is shown >T3894 GGGTAAGATGGCAGCGGACGGACAGTGCTCGCTCCCCGCTTCATGGCGGCCAGTGACCCTCACCCACGTC GAATACCCTGCAGGTGATCTGTCTGGCCACCTCCTGGCCTACCTGAGCCTCAGCCCTATTTTTGTCGTTG TTGGTTTTTTGACTCTCATCATATTCAAGCGGGAGCTACACACGATTTCATTCCTTGGGGGACTGGCACT GAATCAGGGCGTCAACTGGCTGATCAAACACGTCATCCAGGAGCCTCGGCCCTGTGGAGGCCCACATACA GCAGCGGGCACTAAGTACGGGATGCCCTCCAGCCATTCCCAGTTCATGTGGTTCTTCTCCGTCTACTCCT TCCTCTTCCTGTATTTAAGAATGCACCAAACAAATAACGCCAGGTTCTTGGACTTGCTGTGGAGGCACGT GCTGTCTCTAGGGCTCCTCACCGCGGCCTTTCTCGTCTCCTATAGCAGGGTCTACCTGCTGTACCACACC TGGAGCCAGGTGTTTTATGGGGGCGTGGCTGGAAGCCTCATGGCCGTCGCCTGGTTCATCATCACGCAGG AAATCCTCACTCCGTTGTTCCCCAGGATAGCAGCCTGGCCCATCTCCGAGTTTTTCCTCATCCGGGACAC GAGCCTCATTCCCAATGTGCTCTGGTTTGAGTACACAGTAACCAGGGCAGAAGCCAGGAACAGACAGCGC AAACTTGGAACAAAACTGCAGTGACTGGTGGGTGTGGCTGCGTCTAGCCTCCGAATCTGGCCTGCACCAT GCCTTGCAGGATGGACAGAATGATGGATGGAGGGGTGAAGCAGAGACCTCTACAGACCAGAGTCACCAAG TGGAGCCTTTTTTTTTCTTATTTTAATTTCAATGGACACGGTGGACCAAAGTGCTGAGTCAGGTCCTCAG CCAGGACCCTGGGAGGGCCTGCTGTGTGTGGGTCACATGGCCAGAGCCCATCTCTGCTGCTGCCAGCTCC TGGCTGGGTGGAAAGTGCTCTTGGCACGGGCCTCAGCGTGCGTGTAGAGCGTGTGTACCTCTGGTCCTGA GATCAGGAAGTGCAGGTAGCGGAATGCGCACTATTTATTCTTTTGGGATTGTGGAGTTCATGCTGAAGAG GCCACTCTGCAGCAGCTGCCCTGTGTACACAGGGCCCAGGTTAGCTCCCAGACCCTGTGCTCTGAGCCTC AGGCAGTACAGCCTGCCTCCTGCTGGGCCTGAGCAGGGCCTAGGGCCCAGGGCCCAGGAGCGAGGCCTCC CCTGCTGTCCCTGCCACCATCGCCATTCCAGAACCCTTGTCAGTGTTTCCTCGTCTGGGGCAGGGCCCAG AGTCAGCACCTGTTTATCCGTCTGTCGGCTGGGCTGCTGGCTTGTGTTCTTCCCTCTACCAACCCGACAT CACAAGGGCTTTCTCCGGTCCTCTATCCCTTCCTGACAAAAGAGCCTGCACATACGGGTGAGCACACCCA AGCGTTTACAGCTCCTTTTGTCTGGCCATCGGGTAGCAGTCTGTCTTCATCCCCACTTGAACAAAAGAGA GGAAGCAGGAGCTGGGATGCTGGCTGTGTGGCAGTGCAGACACCCTCCCCCATCGCCTGCCCATGTGCTC TGGATTTGTCCCTGTCCTTCCTGGCCTGTCCTGGAGGCTGGAAACCAGTGCAGGTTGATCCTGGGGTCTG CCTGGGAGTGGGCTCTGATCCCAGCTGCCAGGAGTCCCTTTTAAGGGACTCTCTCCAGGTTGTCCTGCCA CAGCTGTGGAGATGCTTCCAGAACTGAAGCAGCCCCTGGGGGAGCTGTTGAGCCCTCCCCCGTTTCTCCC TGGAGGTCCCTAACCTGCTGCTGAAGCACAAGTTTTTGGTGCTTTCCTTTGTGTTTGTTAAAGGCCGTGT CCAAGCCCCTCAGATGCCAGGGCCAGGGCTGGTTTCTAGGGAGGTAGGTCAGTCACATCCATCCCCCCTC CCCCGTAGGATTTTTATTTTTTACTTTTTGCCTGAGACAAGCCAAGTGTGAGGTGGTTTAAGAAATCAAA TGAAATTGTTTACTATTGTTTTAAAATAAAATCTGAAACTCTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4975127 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4975127 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10524 same embryo
	EMAGE:10526 same embryo
	EMAGE:10525 same embryo
	EMAGE:10528 same embryo
	EMAGE:10527 same embryo
	EurExpress:euxassay_000817 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source