EMAGE mouse gene expression spatial database

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EMAGE:10528

Selenbp2 selenium binding protein 2 ( MGI:104859)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000825. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000825_01	euxassay_000825_02	euxassay_000825_03	euxassay_000825_04

euxassay_000825_05	euxassay_000825_06	euxassay_000825_07	euxassay_000825_08	euxassay_000825_09

euxassay_000825_10	euxassay_000825_11	euxassay_000825_12	euxassay_000825_13	euxassay_000825_14

euxassay_000825_15	euxassay_000825_16	euxassay_000825_17	euxassay_000825_18	euxassay_000825_19

euxassay_000825_20	euxassay_000825_21	euxassay_000825_22	euxassay_000825_23	euxassay_000825_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10528_wholemount_strong.wlz

10528_wholemount_moderate.wlz

10528_wholemount_weak.wlz

10528_wholemount_possible.wlz

10528_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10528_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

not examined

homogeneous

not examined expression: see section 12

nasal cavity epithelium

strong

homogeneous

strong expression: see section 16 moderate expression: see section 12 15 17

nasal cavity olfactory epithelium

moderate

homogeneous

moderate expression: see section 11

esophagus epithelium

moderate

homogeneous

moderate expression: see section 14

pharynx epithelium

strong

homogeneous

strong expression: see section 12 13 14 15 16

oral epithelium

strong

homogeneous

strong expression: see section 16 moderate expression: see section 05 09 10 11 12 15 17

liver

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3902
Entity Detected:	Selenbp2, selenium binding protein 2 ( MGI:104859)
Sequence:	sense strand is shown >T3902 ACTTAATACCAGCACTGGTCTCTGCTGAGCCTCTGCTCATTCTTGGCAAATCCGGCAGGACCCGAGTGTC AGCCAGCCAGCAGCATGGCTACAAAATGCACAAAGTGTGGTCCAGGTTACCCCACCCCTCTGGAGGCCAT GAAAGGACCCCGAGAGGAGATTGTCTACTTGCCCTGCATTTACCGGAACACAGGCACCGAAGCCCCGGAT TATTTGGCCACTGTGGATGTAGACCCTAAGTCTCCCCAGTATAGCCAGGTCATCCACAGGTTGCCCATGC CCTACCTGAAGGACGAGCTGCACCACTCAGGATGGAACACCTGCAGCAGCTGCTTTGGGGACAGCACGAA GTCACGCAACAAGCTGATACTGCCTGGTCTCATCTCCTCCCGCATCTACGTGGTGGATGTGGGCTCTGAG CCTCGTGCCCCGAAGTTGCACAAGGTCATTGAAGCCAGTGAAATCCAAGCCAAGTGCAACGTGAGCAATA CGCACACCAGCCACTGCCTGGCCAGTGGAGAAGTCATGGTCAACACCTTGGGGGATCTCCAGGGTAATGG CAAAGGGAGTTTTGTGCTGCTGGATGGGGAGACCTTCGAGGTGAAAGGGACATGGGAGAAGCCTGGGGGT GCATCTCCAATGGGCTACGACTTCTGGTACCAGCCTCGACACAATGTCATGGTTAGCACTGAATGGGCAG CTCCCAATGTCTTCAAAGATGGCTTCAACCCGGCTCATGTGGAGGCTGGGCTGTATGGGAGCCGCATATT TGTGTGGGACTGGCAGCGCCATGAGATTATCCAGACCCTGCAAATGACAGATGGGCTGATACCCCTGGAG ATCCGTTTCCTGCACGACCCAAGTGCCACCCAGGGTTTTGTAGGCTGTGCCCTCAGCTCCAACATCCAGC GCTTCTACAAGAATGAGGAAGGCACTTGGTCAGTGGAGAAGGTGATCCAAGTGCCCTCCAAGAAAGTGAA GGGCTGGATGTTGCCAGAAATGCCTGGTTTGATCACCGACATCCTGCTGTCCCTGGATGACCGCTTCCTT TACTTCAGCAACTGGCTGCATGGGGACATTCGGCAGTATGACATCTCTAACCCACAGAAGCCCCGCCTCA CTGGGCAGATCTTCCTTGGGGGCAGCATTGTTAGAGGAGGCTCTGTGCAAGTGCTGGAGGACCAAGAGTT AACCTGTCAGCCGGAGCCCCTAGTGGTCAAGGGAAAACGAATTCCTGGAGGCCCTCAGATGATCCAGCTC AGCTTAGATGGCAAGCGTCTTTACGCCACTACATCACTGTACAGCGACTGGGACAAGCAGTTTTACCCTG ACCTCATTAGGGAAGGCTCCGTGATGCTTCAAGTTGATGTAGACACAGTAAATGGAGGGCTGAAGTTGAA CCCCAACTTTCTGGTGGACTTTGGGAAGGAGCCCCTTGGGCCAGCACTGGCTCACGAGCTTCGTTACCCA GGGGGTGACTGCAGTTCTGACATCTGGATCTGAAGGCTCACACTAGGGGCCCCCCTCCACATTCTGAGCC TGCCTGGCTTCACTCTGCTCTCAGCCCCAACTCTCCAAGGCCACTCTGAGACCAGCGAGATCTGTAGAGC AGCATCTCTGTACCGTTGCTTGTTGTTTGTGCTTTTCTTAAGTGAGCTCCTGGAAGCACCTGGAAATAAA ATGCTGAAACTTCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5096831 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5096831 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10524 same embryo
	EMAGE:10526 same embryo
	EMAGE:10523 same embryo
	EMAGE:10525 same embryo
	EMAGE:10527 same embryo
	EurExpress:euxassay_000825 same experiment
	MGI:4827942 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source