EMAGE mouse gene expression spatial database

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EMAGE:10530

Ndufb8 NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 ( MGI:1914514)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000734_01	euxassay_000734_02	euxassay_000734_03	euxassay_000734_04	euxassay_000734_05

euxassay_000734_06	euxassay_000734_07	euxassay_000734_08	euxassay_000734_09	euxassay_000734_10

euxassay_000734_11	euxassay_000734_12	euxassay_000734_13	euxassay_000734_14	euxassay_000734_15

euxassay_000734_16	euxassay_000734_17	euxassay_000734_18	euxassay_000734_19	euxassay_000734_20

euxassay_000734_21	euxassay_000734_22	euxassay_000734_23	euxassay_000734_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10530_wholemount_strong.wlz

10530_wholemount_moderate.wlz

10530_wholemount_weak.wlz

10530_wholemount_possible.wlz

10530_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10530_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T951
Entity Detected:	Ndufb8, NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 ( MGI:1914514)
Sequence:	sense strand is shown >T951 TCCTCGAGNCTGTTGGCCTACTGGAAGGTGAAGATGGCTGCCGCCCGGGCTGCGGCCCTGGGAGTCCGAT GGCTGCAGAGGACAACCCGGGGCGTGGTGCCACTGGAGGCACGGAGAGCCTTCCATATGACCAAGGACAT GTTGCCGGGGTCATATCCTAGGACCCCAGAGGAACGCGCCGCGGCCGCCAAGAAGTATAACATGCGAGTG GAAGACTACGAGCCATACCCCGATGATGGCATGGGGTATGGCGACTACCCGATGCTCCCCAACCGATCAC AGCATGAGAGGGATCCGTGGTATCAGTGGGACCACTCAGAACTCAGGATGAACTGGGGTGAACCGATACA CTGGGACCTAGACATGTACATCAGGAATCGTGTGGACACGTCACCTACCCCTGTGTCCTGGGATGTCATG TGTAAACATCTCTTCGGCTTTGTGGCTTTCATGGTTTTCATGTTCTGGGTAGGGCACGTGTTCCCTTCCT ACCAGCCTGTGGGTCCGAAGCAGTACCCTTACAATAATCTGTACCTGGAGCGGGGTGGTG
Notes:	The probe template was PCR amplified from IMAGE:1970237 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1970237 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10533 same embryo
	EMAGE:10532 same embryo
	EMAGE:10531 same embryo
	EMAGE:10529 same embryo
	EMAGE:10534 same embryo
	EurExpress:euxassay_000734 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source