EMAGE mouse gene expression spatial database

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EMAGE:10581

Tmem8 transmembrane protein 8 (five membrane-spanning domains) ( MGI:1926283)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000829. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000829_01	euxassay_000829_02	euxassay_000829_03	euxassay_000829_04

euxassay_000829_05	euxassay_000829_06	euxassay_000829_07	euxassay_000829_08	euxassay_000829_09

euxassay_000829_10	euxassay_000829_11	euxassay_000829_12	euxassay_000829_13	euxassay_000829_14

euxassay_000829_15	euxassay_000829_16	euxassay_000829_17	euxassay_000829_18	euxassay_000829_19

euxassay_000829_20	euxassay_000829_21	euxassay_000829_22	euxassay_000829_23	euxassay_000829_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10581_wholemount_strong.wlz

10581_wholemount_moderate.wlz

10581_wholemount_weak.wlz

10581_wholemount_possible.wlz

10581_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10581_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3936
Entity Detected:	Tmem8, transmembrane protein 8 (five membrane-spanning domains) ( MGI:1926283)
Sequence:	sense strand is shown >T3936 CACCGCAGGCAGAAGGCGCATTTTCTACTTTGGCTAAGGACAAAGTTCAGGGTGGGACAAGTACTTCCTG GAGCCCATGCTTCCTGTGTGCCTGCCCCACCTACCTGATTGTCACCTTTTCCCTGTAGAGGCTGGACTGG TATCTGAACACTTCTCCCAGGCCCCACAGAAGCTGTCCTTCTACAGCTGGTATGGCAGCACCCGGCTCTT CCACTTCCGCGTGCCCCCGGACACTGTGCTGCTCCGCTGGCTGCTGCACGTGTCCCAGGGAGGTCCCTCA TGCACTGACGAAGAGATCACAGTGCATTTCCGCTATGGCGCCCCTCCTGTCATCAACCCTCTGGGCACCA GCTTCCCTGACAACACCTTGAGCCACGCCTCCTTCCATATCAGGGCATTGTTGAGCACCTTGATGCTGGA CAACACCTCAGTCAACATCTCCCACCCTGCCCCTGGGGCCTGGTTCCTGGTTGCTCACCTCCCTCCCTCC TCCCAGAAGATCCAGGTGAAGGGCTTTGTTCCAACCTGTGCCTACATCTTCCAGCCTGATATGCTGGTAA TGAGGGTGGTTGAAGTCTCCACCCTAGAGCCTGATGTTCCCCTCCCACAGACGCTGCTCTCCTACCCCAG CTACCTCAAGATCTTTGTCCCTGAATACACCCAGGAGCTTCGGTTGGAACTGCAGGGCTGTGTGTCCAGT GTGAGCCCCGGCTGCCCAGTACGTGTCACAGTGGGGGCAACCACCCTGCCTAGAAACTTCCAGAGGGTGC TGACTTGCACTGGCCTCGCTCCATCCTGCCATTTGCTACTGTCCTCTCCACCCTGGGGCCGGTGGTTACA AGTGACATTTGAGAGCCTGGCTGAACCTCATGTGACAGTGGGCTTTACTGCTAAAGCTGTCTTTACAGTA TGTAGGCCATGGAGTGTGACCATCCATCATCTTATACAAAACAACCCAAACCAGACTTATGATACCTCTG CAATCCAGCTGTCCCAAAGCGCTGTCCACCGGGACCTGGGCAGGAGTAGCAGGGTGGACAGTGGCCCCTT CTGTCTCCTGAACTATCCAGTCCTGAGGGAGGACACAGATGTGGTGTCTGTACACTTCCAGCCCCTGAAT GGGGCCTTCGTGCTAGTGCATTCAAGCATGCCTTCTGTAATGCAGCTCCGCCTTGATACAGGCATGGACA GCGGAGGCTCCTTCATCATCGTCCTGAGGACTAACAAGACAGAGGTCACAAATGGCACCTTGGTAGCAGC TTGTGTGAATGCTGCCTCCCCTTTCCTCAGCTTCAACACTTCACTCAACTGCACCACAGCCTTTTTCCAG GGCTACCCCATGTTTCTGAGAGCCTCATCTCACATGGCCAACCTCATCATGCCCTTCCCGGAGACAGACA ACTGGTACCTCTCCCTGCAGCTTGTGTGCCCTGAGAGTCCTGAGGATTGTGAACAGGCTGTGGTTCGCGT GGAGACCATCTTGTATCTGGTGCCCTGTTTGAATGACTGTGGGCCCTACGGCCAGTGTCTCCTGCTCCGT AGATACGGCTATGTGTACGCAGGCTGCAGCTGCAAGGCAGGTTGGCGTGGGTGGAGCTGCACCGACAACA GCACAGCGCAGACCGTAGCCCAGCAGAGGGCAGCAGCGCTTCTGCTCACGCTCAGCAACCTCATGTTCCT GGCCCCCATTGCTATATCCCTGCACCGTTCCTTCCTGGTCGAGGCCTCTGTCTACTTTTACACCATGTTC TTCTCCACGTTCTACCACGCCTGTGACCAACCCGGGGAGGCAGTGTTGTGCATCCTCAGTTATGACACGC TACAGTATTGCGACTTTCTGGGCTCTGGGGCGTCCACCTGGGTGACCATTCTTTGCATGGCCCGTCTGAA GACAATCCTGAAACAGGTACTACTTGTCCTCGGCACACTCGTCATCGCCATGTCCTTGCAGATGGATCGC AGGGGCATCTGGAACTTGATGGGACCTTGCGTCTTTGCTTTTGTGATCATGGCCTCCATGTGGATATACC GCTGTGGGCACCGGGGACAGTGCTACCCAACCTCCTGGCAGCGCTGGGTCTTCTACCTCCTGCCTGGCAT CTCCATGGCTTCCGTTGGCATCGCCATGTATACTTCAATGATGACCAGTGACAATTACTACTACACCCAC AGCATTTGGCACATCCTACTGGCTGGTAGTGCAGCATTTCTTCTGCCACCACGAGAGGAAAAAGCTGGGT CCTGGGCCTGTTTGCAGAAGTTCCCTTGTCACTACCAGATCTGCAGGAATGATCGGGATGAGTTGTACAC AGTGACCTGATAAGGCTGGTTCATGGACACCTGAAACTCTAATGACCTCTTCAGCTAGGACAAGCGAGAG AGGTGCTTGTCTAGCCCCAACCAGGTTTCCATCTGAATAAAATTGTCCTGAGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3595205 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3595205 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10582 same embryo
	EMAGE:10584 same embryo
	EMAGE:10586 same embryo
	EMAGE:10583 same embryo
	EMAGE:10585 same embryo
	EurExpress:euxassay_000829 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source